Plant Genetic Techniques

Rajcan, Istvan; Boersma, Jeffrey G.; Shaw, Eric

doi:10.1016/b978-0-08-088504-9.00252-x

Cited by 9 publications

(1 citation statement)

References 33 publications

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“…The response was dependent on the rye genotype used. Haploid zygotic embryos that differentiate into fully developed plants had been generated from wide (interspecific/intergeneric) crosses due to a rapid and complete elimination of the genome of the male parent right after pollination (Rajcan et al, 2011). The use of this method for inducing doubled haploids for breeding staple crops is relevant for cassava.…”

Section: Discussionmentioning

confidence: 99%

Vibratome Sectioning and Clearing for Easing Studies of Cassava Embryo Formation

2020

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This work describes the application of clearing on vibratome sections to study the embryo formation in cassava. This procedure provides high-resolution images and reduces significantly the number of sections that need to be analyzed per ovule. This methodology was instrumental for the development of the protocol for embryo rescue in cassava. It has been also applied to monitor the embryo formation response when optimizing seed setting from regular and broad crosses for cassava breeding. Broad crosses between cassava and castor bean (incompatible-euphorbiaceae species) were made aiming to induce doubled haploids through the elimination of the incompatible-male parent genome as done in cereals. Castor bean is widely available and provides continues supply of pollen. Our results suggest that this methodology is easy and effective to assess the response of hundreds of cassava ovules pollinated with castor bean pollen, allowing the identification of multicellular structures in the embryo sac without apparent formation of endosperm. The protocol is also useful when developing and optimizing a methodology to induce doubled haploids in cassava via gynogenesis or from ovules pollinated with irradiated cassava pollen.

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Section: Discussionmentioning

confidence: 99%