Plant glutathione transferase-mediated stress tolerance: functions and biotechnological applications

Nianiou-Obeidat, Irini; Madesis, Panagiotis; Kissoudis, Christos; Voulgari, Georgia; Chronopoulou, Evangelia; Tsaftaris, Athanasios; Labrou, Nikolaos E.

doi:10.1007/s00299-017-2139-7

Cited by 197 publications

(126 citation statements)

References 139 publications

Supporting

Mentioning

125

Contrasting

Order By: Relevance

“…These include tau (U), phi (F), lambda (L), theta (T), and zeta (Z) GSTs, as well as dehydroascorbate reductases (DHARs), tetrachlorohydroquinone dehalogenases (TCHQDs), g-subunits of the eukaryotic translation elongation factor 1B (EF1Bg), Ure2p, and microsomal prostaglandin E synthases type 2 (mPGES-2). The 14 classes also include recently identified classes such as hemerythrin (H), iota (I) GSTs, and glutathionyl-hydroquinone reductases (GHRs) [20][21][22][23][24][25]. The plant-specific tau class GSTs are the most numerous GST class in plants, and they are essential for plants to resist biotic or abiotic stresses [4].…”

Section: Introductionmentioning

confidence: 99%

Effects of Substrate-Binding Site Residues on the Biochemical Properties of a Tau Class Glutathione S-Transferase from Oryza sativa

Yang

Wei

et al. 2019

Genes

View full text Add to dashboard Cite

Glutathione S-transferases (GSTs)—an especially plant-specific tau class of GSTs—are key enzymes involved in biotic and abiotic stress responses. To improve the stress resistance of crops via the genetic modification of GSTs, we predicted the amino acids present in the GSH binding site (G-site) and hydrophobic substrate-binding site (H-site) of OsGSTU17, a tau class GST in rice. We then examined the enzyme activity, substrate specificity, enzyme kinetics and thermodynamic stability of the mutant enzymes. Our results showed that the hydrogen bonds between Lys42, Val56, Glu68, and Ser69 of the G-site and glutathione were essential for enzyme activity and thermal stability. The hydrophobic side chains of amino acids of the H-site contributed to enzyme activity toward 4-nitrobenzyl chloride but had an inhibitory effect on enzyme activity toward 1-chloro-2,4-dinitrobenzene and cumene hydroperoxide. Different amino acids of the H-site had different effects on enzyme activity toward a different substrate, 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole. Moreover, Leu112 and Phe162 were found to inhibit the catalytic efficiency of OsGSTU17 to 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, while Pro16, Leu112, and Trp165 contributed to structural stability. The results of this research enhance the understanding of the relationship between the structure and function of tau class GSTs to improve the abiotic stress resistance of crops.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Substrate-Binding Site Residues on the Biochemical Properties of a Tau Class Glutathione S-Transferase from Oryza sativa

Yang

Wei

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…The significant upregulation of genes associated with PR-1 protein, glutathione-S-transferase, phospholipid hydroperoxide glutathione peroxidase, peroxidase was observed particularly at 48 hpi which are reported to minimize the oxidative damage in plants in response to biotic and abiotic stresses 62,63 . Homologs of these gene products restrict the oxidative damage in host tissue and initiate several succeeding downstream signaling cascades 62,64 . The PR-1 gene has been thoroughly studied in the past for its role in defense to different pathogens, including P. infestans 65,66 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew

Kandel

Hulse‐Kemp

Stoffel

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Downy mildew of spinach is caused by the obligate oomycete pathogen, Peronospora effusa. the disease causes significant economic losses, especially in the organic sector of the industry where the use of synthetic fungicides is not permitted for disease control. New pathotypes of this pathogen are increasingly reported which are capable of breaking resistance. In this study, we took advantage of new spinach genome resources to conduct RNA-seq analyses of transcriptomic changes in leaf tissue of resistant and susceptible spinach cultivars Solomon and Viroflay, respectively, at an early stage of pathogen establishment (48 hours post inoculation, hpi) to a late stage of symptom expression and pathogen sporulation (168 hpi). Fold change differences in gene expression were recorded between the two cultivars to identify candidate genes for resistance. In Solomon, the hypersensitive inducible genes such as pathogenesis-related gene PR-1, glutathione-S-transferase, phospholipid hydroperoxide glutathione peroxidase and peroxidase were significantly up-regulated uniquely at 48 hpi and genes involved in zinc finger CCCH protein, glycosyltransferase, 1-aminocyclopropane-1-carboxylate oxidase homologs, receptor-like protein kinases were expressed at 48 hpi through 168 hpi. The types of genes significantly up-regulated in Solomon in response to the pathogen suggests that salicylic acid and ethylene signaling pathways mediate resistance. Furthermore, many genes involved in the flavonoid and phenylpropanoid pathways were highly expressed in Viroflay compared to Solomon at 168 hpi. As anticipated, an abundance of significantly down-regulated genes was apparent at 168 hpi, reflecting symptom development and sporulation in cultivar Viroflay, but not at 48 hpi. In the pathogen, genes encoding RxLR-type effectors were expressed during early colonization of cultivar Viroflay while crinkler-type effector genes were expressed at the late stage of the colonization. Our results provide insights on gene expression in resistant and susceptible spinach-P. effusa interactions, which can guide future studies to assess candidate genes necessary for downy mildew resistance in spinach.The demand for prepackaged ready to eat salad mixes has resulted in increased production of leafy greens in recent years. Currently, spinach is cultivated in more than 60 countries globally, with over 53 million tons of total production annually 1 . In the US, nearly four hundred thousand tons of spinach are produced every year 1 . There has also been a rapid increase in the demand for spinach in the US, beginning in the 1990s 2,3 . The Salinas Valley of California is sometimes referred to as 'Salad Bowl of America' since this region produces the majority of leafy greens grown in the US as well as nearly half of the total spinach in California 4,5 .Downy mildew disease of spinach is caused by the obligate oomycete pathogen Peronospora effusa (Phylum Oomycota, Kingdom Stramenopila) 6 . Downy mildew on spinach can sometimes cause 100% yield loss in organic production s...

show abstract

“…The central position in the GSH network is occupied by GST (Labrou et al, 2015). Glutathione S-transferases play important roles in stress tolerance along with other protective functions in plants (Nianiou-Obeidat et al, 2017). In this work, a four-fold increase in GST activity was determined against 1B treatment.…”

Section: Primer Namementioning

confidence: 95%

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

Kayıhan¹,

Kayıhan²,

Çiftçi³

2019

Turk J Bot

View full text Add to dashboard Cite

The fine-tuned regulation of the Halliwell-Asada cycle (ascorbate-glutathione pathway) in Arabidopsis thaliana under boron (B) toxicity was shown in our previous report. In this study, we investigated the expression levels of some members of the glutathione S-transferase (GST) superfamily, such as phi (GSTF2, GSTF6, GSTF7, and GSTF8), tau (GSTU19), and zeta (GSTZ1) classes in Arabidopsis thaliana that were exposed to 1 mM boric acid (1B) and 3 mM boric acid (3B). Additionally, the expression levels of genes for glutathione (GSH) and phytochelatin biosynthesis as well as miR169 and miR156 were evaluated in Arabidopsis thaliana exposed to 1B and 3B. Moreover, changes in the levels of total GST activity; GSH; and total, protein-bound, and nonprotein thiols were spectrophotometrically determined. GSH levels and nonprotein thiol content did not change significantly following both B-toxicity conditions. Expression levels of GSH1 and GSH2 stayed stable under 1B toxicity; however, GSH1 expression increased significantly under 3B conditions in Arabidopsis thaliana. The expression levels of four genes from phi class members of GST were not dramatically changed under B-toxicity conditions. However, the transcript levels of miR169, ATGSTU19, and ATGSTZ1 were significantly increased after 1B and 3B exposure. These GST genes may have a role in the dramatic increase of total GST activity under toxic B. To the best of our knowledge, this is the first report displaying an integrative view of high-B-induced regulation of GSH-dependent enzymatic machinery at different biological organization levels in Arabidopsis thaliana.

show abstract

Plant glutathione transferase-mediated stress tolerance: functions and biotechnological applications

Cited by 197 publications

References 139 publications

Effects of Substrate-Binding Site Residues on the Biochemical Properties of a Tau Class Glutathione S-Transferase from Oryza sativa

Effects of Substrate-Binding Site Residues on the Biochemical Properties of a Tau Class Glutathione S-Transferase from Oryza sativa

Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew

Regulation of boron toxicity responses via glutathione-dependent detoxification pathways at biochemical and molecular levels in Arabidopsisthaliana

Contact Info

Product

Resources

About