Plant Glycoprotein Modulates the Expression of Interleukin-1β<i>via</i>Inhibition of MAP Kinase in HMC-1 Cells

Oh, Phil-Sun; Lim, Kye-Taek

doi:10.1271/bbb.80204

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…The tuber storage protein of Dioscorea alata, dioscorin (Hsu et al, 2002), and red mold Dioscorea (Wu et al, 2009) exhibit angiotensin-converting enzyme inhibitory activities. Moreover, some Dioscorea species and their constituents are known to have anti-inflammatory properties (Oh and Lim, 2008;Hiransai et al, 2010;Nguelefack et al, 2010;Mbiantcha et al, 2011). Considering the previous references, the inhibitory activities of DA-9801 on diabetic neuropathy may be exerted by multi-targeted pharmacological activities of the Dioscorea species.…”

Section: Discussionmentioning

confidence: 92%

Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice

Moon¹,

Lee²,

Kang³

et al. 2014

Biomol Ther

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. www.biomolther.orgThe purpose of this study was to investigate the therapeutic effects of DA-9801, an optimized extract of Dioscorea species, on diabetic peripheral neuropathy in a type 2 diabetic animal model. In this study, db/db mice were treated with DA-9801 (30 and 100 mg/kg, daily, p.o.) for 12 weeks. DA-9801 reduced the blood glucose levels and increased the withdrawal latencies in hot plate tests. Moreover, it prevented nerve damage based on increased nerve conduction velocity and ultrastructural changes. Decrease of nerve growth factor (NGF) may have a detrimental effect on diabetic neuropathy. We previously reported NGF regulatory properties of the Dioscorea genus. In this study, DA-9801 induced NGF production in rat primary astrocytes. In addition, it increased NGF levels in the sciatic nerve and the plasma of type 2 diabetic animals. DA-9801 also increased neurite outgrowth and mRNA expression of Tieg1/Klf10, an NGF target gene, in PC12 cells. These results demonstrated the attenuation of diabetic peripheral neuropathy by oral treatment with DA-9801 via NGF regulation. DA-9801 is currently being evaluated in a phase II clinical study.

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Section: Discussionmentioning

confidence: 92%

Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice

Moon¹,

Lee²,

Kang³

et al. 2014

Biomol Ther

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“…Preparation of cell extract was basically as described elsewhere . Briefly, the cells and liver lysates were washed in 1 ml of ice‐cold PBS after the medium was removed, and scraped into 300 µl of ice‐cold buffer C [20 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES), pH 7.9, 0.4 M NaCl, 0.2 mM EDTA, 1 mM dithiothreitol (DTT), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1.5 mM MgCl 2 , 0.5% NP‐40, 25% glycerol] containing a phosphatase inhibitor cocktail.…”

Section: Methodsmentioning

confidence: 99%

Preventive effects of ZPDC glycoprotein (24 kDa) on hepatotoxicity induced by mercury chloride in vitro and in vivo

Liu

Lee

Lim

2014

Cell Biochemistry & Function

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Mercury is a potent environmental contaminant that exerts toxic effect on various vital organs in the human body. Recently, we isolated glycoprotein from Zanthoxylum piperitum DC (ZPDC), which has antioxidant and anticancer effects. In the present study, we determined the preventive effects of ZPDC glycoprotein on hepatic damage induced by mercury chloride (HgCl2 ). We evaluated the activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)], extracellular signal-regulated kinase (ERK)1/2, p38 mitogen-activated protein kinase (MAPK), cyclo-oxygenase (COX-2), inducible nitric oxide synthetase (iNOS), and activator protein (AP-1) and the quantitative expressions of nuclear factor E2-related factor (Nrf2), heme oxygenase (HO-1), metallothionein (MT) and reduced glutathione (GSH) in mercury-chloride-exposed (50 μM and 10 mg/kg body weight) primary cultured hepatocytes and ICR mice, using biochemical assays, radioactivity and immunoblot analysis. The results demonstrated that ZPDC glycoprotein decreased the levels of LDH, ALT, HO-1 and MT, whereas it increased the activities of hepatic antioxidant enzymes (SOD, CAT and GPx) and reduced GSH in mercury-chloride-exposed primary cultured hepatocytes. Also, it suppressed arachidonic acid release and expression of ERK, p38 MAPK, COX-2, iNOS, AP-1 and Nrf-2 in primary cultured hepatocytes and ICR mice exposed to mercury chloride. Collectively, ZPDC glycoprotein may have potential applications to prevent hepatotoxicity induced by mercury chloride.

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“…Cell extracts were prepared as described previously [21]. The cells were washed in 1 ml ice-cold PBS after the medium was removed, and scraped into 300 μl of ice-cold buffer C (20 mM HEPES, pH 7.9, 0.4 M NaCl, 0.2 mM EDTA, 1 mM DTT, 1 mM PMSF, 1.5 mM MgCl 2 , 0.5% NP-40, and 25% glycerol) containing a phosphatase inhibitor cocktail.…”

Section: Preparation Of Cell Extractsmentioning

confidence: 99%

SJSZ glycoprotein (38 kDa) prevents thymus atrophy and enhances expression of IL-2 and IL-12 in diethylnitrosamine-induced hepatocarcinogenesis

Liu

Lim

2012

International Immunopharmacology

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Plant Glycoprotein Modulates the Expression of Interleukin-1βviaInhibition of MAP Kinase in HMC-1 Cells

Cited by 16 publications

References 25 publications

Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice

Dioscorea Extract (DA-9801) Modulates Markers of Peripheral Neuropathy in Type 2 Diabetic db/db Mice

Preventive effects of ZPDC glycoprotein (24 kDa) on hepatotoxicity induced by mercury chloride in vitro and in vivo

SJSZ glycoprotein (38 kDa) prevents thymus atrophy and enhances expression of IL-2 and IL-12 in diethylnitrosamine-induced hepatocarcinogenesis

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