Plant growth-promoting <i>Burkholderia</i>
 species isolated from annual ryegrass in Portuguese soils

Castanheira, Nádia; Dourado, Ana Catarina; Kruz, S.; Alves, Paula C.; Delgado-Rodríguez, A.I.; Pais, Isabel P.; Semedo, José N.; Campos, Paula Scotti; Lara, Claudia; Borges, Nuno; Carvalho, Gilda; Crespo, Maria Teresa Barreto; Fareleira, Paula

doi:10.1111/jam.13025

Cited by 48 publications

(30 citation statements)

References 77 publications

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“…Strain T 2-32 (Ensifer adhaerens) would be an interesting strain to test in further experiments because of its stunting IAA production (1170.98 µg mg protein -1 ) compared to A. brasilense AbV5 (commercial inoculant) or H. seropedicae (positive control) (Table 1). Among the isolates, Agrobacterium, Bacillus and Burkholderia were considered the strongest P solubilizers; this result has been confirmed by others (Rodríguez & Fraga 1999;Castanheira et al, 2016;Moreira et al, 2016). In vitro strain selection may contribute to a better screening of potential biofertilizer strains, especially in gnotobiotic conditions, where environmental aspects could mask plant and bacterial interactions (Khalid et al, 2004;Fürnkranz et al, 2009).…”

Section: Discussionsupporting

confidence: 55%

Interactions between endophytic bacteria and their effects on poaceae growth performance in different inoculation and fertilization conditions

Chaves¹,

Guimarães²,

Vendruscolo³

et al. 2019

Aust J Crop Sci

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Plant growth-promoting bacteria (PGPB) are a broad group of microorganisms that offer a huge, unexplored potential. We assessed the genetic diversity of endophytic bacteria that were isolated from corn and wheat plants in 3 different types of soils: A1 (Red Argisol), A2 (Red Latosol) and A3 (Red Nitossol). Endophytic bacteria were isolated from seedlings using 7 solid culture media and were further analyzed by 16S gene sequencing. IAA (Indole-3-acetic acid) production and PSI (Phosphorus solubilization index) bacteria capacity were tested in a randomly scheme in triplicate. In vitro association was assayed with 6 randomly selected endophytic strains. The experiment design was arranged in a randomly scheme with 10 replications. In vivo assays were performed to evaluate plant growth promotion using a low-fertility soil in different inoculation and/or fertilization conditions. The experiment design was arranged in a factorial 3x4 scheme in triplicate. The following characteristics were evaluated in both experiments in triplicate: plant biomass, total nitrogen content (TN) and endophytic population. As results, a total of 136 isolates were collected, and from these isolates, 41 strains were sequenced and classified into 4 major phylogenetic categories. There was a 38-fold variation between the highest (Ensifer adhaerens) and the lowest (Agrobacterium larrymoorei) IAA producer, and only 14% of strains were high phosphate solubilizers. In vitro assays identified both positively (Burkholderia ambifaria) and negatively (Pantoea ananatis) associative strains. In vivo assays showed that plant genotype can limit or induce the endophytic microbiota and that plant microbiota are highly influenced by soil fertility.

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Section: Discussionsupporting

confidence: 55%

Interactions between endophytic bacteria and their effects on poaceae growth performance in different inoculation and fertilization conditions

Chaves¹,

Guimarães²,

Vendruscolo³

et al. 2019

Aust J Crop Sci

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“…have been previously shown to confer growth promoting abilities to plants and were shown to increase the productivity of a wide variety of crops (Oteino et al, 2015;Devi et al, 2015, Sharma et al, 2014and Castanheira et al, 2016. Favaro et al (2012) investigated the antagonistic potential of the sugarcane endophytic Epicoccum nigrum P16 strain against Ceratocyctis.…”

Section: Resultsmentioning

confidence: 99%

Analysis of plant growth promoting potential of endophytes isolated from echinacea purpurea and lonicera japonica

Gupta

Saini

Pagadala

et al. 2016

J. Soil Sci. Plant Nutr.

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Plant endophytes help in maintaining plant health by means of their biofertilizer and biocontrol attributes and, are currently being explored for their ability to produce novel biologically active compounds. Herein, we have isolated beneficial endophytic bacteria from Echinacea purpurea (EF.B3) and Lonicera japonica (LS.B11) that showed phosphate solubilization, siderophore, indole acetic acid and hydrogen cyanide production, and fixation of atmospheric nitrogen. Additionally, the endophytes also conferred antifungal activity against Fusarium sp., Rhizoctonia sp., Pythium sp. and Alternaria sp. When tested in plantae, the LS.B11 and EF.B3 strains were able to promote plant growth and control fungal infections in pea seedlings. Both strains were found to be endophytic as tested by RAPD and viability count. Based on 16S rRNA gene sequencing, we show that the LS.B11 and EF.B3 strains are related to Pseudomonas sp. and Burkholderia sp. By using degenerate primers, we identified genes related to polyketide synthases and non-ribosomal peptide synthetases in EF.B3 and LS.B11, respectively that are typically involved in the production of antimicrobial compounds. Therefore, we conclude that both endophytes can be used for increasing agriculture productivity and in the production of antimicrobial compounds for crop improvement.

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“…Some induce plant leaf and root nodule formation and can promote plant growth by the production of auxins [ 27 ]. Some species of Burkholderiales are associated with plants and are known to have growth-promoting effects [ 28 ]. Burkholderia species are common rhizosphere constituents of major agricultural crops including maize, rice, sugarcane, wheat, tomato, and potato.…”

Section: Discussionmentioning

confidence: 99%

Apple endophytic microbiota of different rootstock/scion combinations suggests a genotype-specific influence

Liu¹,

et al. 2018

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BackgroundHigh-throughput amplicon sequencing spanning conserved portions of microbial genomes (16s rRNA and ITS) was used in the present study to describe the endophytic microbiota associated with three apple varieties, “Royal Gala,” “Golden Delicious,” and “Honey Crisp,” and two rootstocks, M.9 and M.M.111. The objectives were to (1) determine if the microbiota differs in different rootstocks and apple varieties and (2) determine if specific rootstock-scion combinations influence the microbiota composition of either component.ResultsResults indicated that Ascomycota (47.8%), Zygomycota (31.1%), and Basidiomycota (11.6%) were the dominant fungal phyla across all samples. The majority of bacterial sequences were assigned to Proteobacteria (58.4%), Firmicutes (23.8%), Actinobacteria (7.7%), Bacteroidetes (2%), and Fusobacteria (0.4%). Rootstocks appeared to influence the microbiota of associated grafted scion, but the effect was not statistically significant. Pedigree also had an impact on the composition of the endophytic microbiota, where closely-related cultivars had a microbial community that was more similar to each other than it was to a scion cultivar that was more distantly-related by pedigree. The more vigorous rootstock (M.M.111) was observed to possess a greater number of growth-promoting bacterial taxa, relative to the dwarfing rootstock (M.9).ConclusionsThe mechanism by which an apple genotype, either rootstock or scion, has a determinant effect on the composition of a microbial community is not known. The similarity of the microbiota in samples with a similar pedigree suggests the possibility of some level of co-evolution or selection as proposed by the “holobiont” concept in which metaorganisms have co-evolved. Clearly, however, the present information is only suggestive, and a more comprehensive analysis is needed.Electronic supplementary materialThe online version of this article (10.1186/s40168-018-0403-x) contains supplementary material, which is available to authorized users.

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Plant growth-promoting Burkholderia species isolated from annual ryegrass in Portuguese soils

Abstract: The novel strain belongs to the plant-associated Burkholderia cluster and is a promising candidate for exploitation as plant inoculant in field conditions.

Cited by 48 publications

References 77 publications

Interactions between endophytic bacteria and their effects on poaceae growth performance in different inoculation and fertilization conditions

Interactions between endophytic bacteria and their effects on poaceae growth performance in different inoculation and fertilization conditions

Analysis of plant growth promoting potential of endophytes isolated from echinacea purpurea and lonicera japonica

Apple endophytic microbiota of different rootstock/scion combinations suggests a genotype-specific influence

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