Plant growth-promoting rhizobacteria as an alternative to mineral fertilizers in assisted bioremediation - Sustainable land and waste management

Grobelak, Anna; Kokot, Paulina; Hutchison, Dylan; Grosser, Anna; Kacprzak, Małgorzata

doi:10.1016/j.jenvman.2018.08.075

Cited by 45 publications

(17 citation statements)

References 24 publications

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“…Plant growth promoting rhizobacteria (PGPR) consisting of a group of beneficial bacteria found in the rhizosphere and rhizoplane of the plants, were proven to be the most environmentally friendly and a better alternative to synthetic agrochemicals and other conventional agricultural practices in augmenting growth and stress tolerance in plants, as well as in attaining sustainable agriculture (Shrivastava and Kumar, 2015; Turan et al, 2017; Gouda et al, 2018; Grobelak et al, 2018; Nagargade et al, 2018). They influence the plant growth both directly and indirectly via various mechanisms like nitrogen fixation, production of plant growth hormone (auxins, cytokinin, and gibberellins), solubilization of phosphates and sequestration of iron by production of siderophores (Bhattacharyya and Jha, 2012; Egamberdieva and Lugtenberg, 2014; Shameer and Prasad, 2018).…”

Section: Introductionmentioning

confidence: 99%

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

2019

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Plant growth promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity has the potential to promote plant growth and development under adverse environmental conditions. In the present study, rhizobacterial strains were isolated from Garlic ( Allium sativum ) rhizosphere and were screened in vitro ACC deaminase activity in DF salt minimal media supplemented with 3 mM ACC. Out of six isolates, two could degrade ACC into α-ketobutyrate, exhibiting ACC deaminase activity producing more than ∼1500 nmol of α-ketobutyrate mg protein -1 h -1 , and assessed for other plant growth promoting (PGP) functions including indole acetic acid production (greater than ∼30 μg/ml), siderophore, Ammonia, Hydrogen cyanide production and inorganic Ca 3 (PO 4 ) 2 (∼85 mg/L) and ZnSO 4 solubilization. Besides facilitating multifarious PGP activities, these two isolates augmented in vitro stress tolerance in response to 6% w/v NaCl salt stress and drought stress (-0.73 Mpa). The strains ACC02 and ACC06 were identified Aneurinibacillus aneurinilyticus and Paenibacillus sp., respectively on the basis of 16S rDNA gene sequence analysis and were evaluated for growth promoting potential in French bean seedlings under non-saline and salinity stress conditions through pot experiments. The seed bacterization by ACC02 and ACC06 revealed that treatment of plants with bacterial isolates in the form of consortia significantly declined (∼60%) stress stimulated ethylene levels and its associated growth inhibition by virtue of their ACC deaminase activity. The consortia treatment alleviated the negative effects of salinity stress and increased root length (110%), root fresh weight (∼45%), shoot length (60%), shoot fresh weight (255%), root biomass (220%), shoot biomass (425%), and total chlorophyll content (∼57%) of French bean seedlings subjected to salinity stress.

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Section: Introductionmentioning

confidence: 99%

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

2019

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“…This work reveals for the first time the positive effect of Pb on bacteria responses. Strain selection in the literature is widely performed without taking into account metals (Plociniczak et al 2018;Grobelak et al 2018). Although bacteria responses under metal stress have been tested, this was done at very high concentrations, e.g., between 25 and 400 mg/L (Rizvi and Khan 2017) and 100 and 500 mg/L (Kumar 2019).…”

Section: Effect Of Pb On Bacterial Degradation Of Aminocyclopropane Cmentioning

confidence: 99%

“…Bacteria increase plant biomass using several mechanisms. For instance, plant nutrients availability in soils is increased by phosphate solubilization or by the production of chelating agents such as siderophores and weak organic acids (Weyens et al 2014;Grobelak et al 2018). Some bacteria produce also indole acetic acid (IAA), a phytohormone stimulating plant growth, or enzymes such as aminocyclopropane carboxylic acid (ACC) deaminase.…”

Section: Introductionmentioning

confidence: 99%

A new assay of bacterial selection with Pb reveals an unexpected effect of Pb on bacterial behavior: implications for remediation

et al. 2020

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Soil pollution by lead (Pb) is a major health concern due to Pb toxicity. Phytoextraction could remove Pb, but this technique is limited by the low Pb mobility in soils. Pb mobility can be increased by bioaugmentation, which consists in adding selected bacteria in soil to increase Pb bioavailability. Nonetheless, many bioaugmentation and phytoextraction experiments have failed because bacterial selections did not take into account the presence of metals. Therefore, we developed a microplate assay with Pb-enriched growth media for the rapid selection of bacterial strains. Selection criteria included the ability of bacteria to grow in soils, to promote plant growth and to increase Pb availability. Results show that 100-250 µM Pb induced a decrease of production of indole acetic acid (IAA), a plant growth promotor, by up to 49% for Cupriavidus metallidurans, compared to the control without Pb. This finding implies that application in real soil conditions with C. metallidurans would have probably failed, thus strengthening the value of our selection method in the presence of Pb. By contrast, 100-250 µM Pb induced a 8.5-11-fold higher degradation of aminocyclopropane carboxylic acid (ACC) by C. metallidurans. Surprisingly, Pseudomonas putida did not degrade ACC without Pb, but degraded ACC at 100-250 µM Pb. This observation means that Pb activates ACC degradation, which should reduce plant stress because ACC is the precursor of the ethylene phytohormone. Overall, our selection method in the presence of Pb allows to reveal new bacterial properties, which would not have been disclosed by current methods that do not take into account the effect of metals. Our method allows also to test simultaneously about 200 bacterial isolates. In addition, our findings show for the first time that Pb changes the production of IAA and the degradation of ACC.

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“…The interaction between bacteria and plants involves complex recognition and signaling mechanisms that activate specific responses (Dennis, Miller & Hirsch, 2010;Sasse, Martinoia & Northen, 2018). Among plant-associated microorganisms, the importance of plant growth-promoting bacteria (PGPB) in agriculture has been increasing, reducing the demand for industrialized fertilizers (Grobelak et al, 2018;Nkebiwe, Weinmann & Müller, 2016). PGPB may interact with plants by colonizing the rhizosphere or the surfaces of leaves and roots or by establishing endophytic colonization (Lery et al, 2008;Zúñiga et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus

Santos

Leandro

Maia

et al. 2020

PeerJ

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Background Plants interact with a variety of microorganisms during their life cycle, among which beneficial bacteria deserve special attention. Gluconacetobacter diazotrophicus is a beneficial bacterium able to fix nitrogen and promote plant growth. Despite its biotechnological potential, the mechanisms regulating the interaction between G. diazotrophicus and host plants remain unclear. Methods We analyzed the response of G. diazotrophicus to cocultivation with Arabidopsis thaliana seedlings. Bacterial growth in response to cocultivation and plant exudates was analyzed. Through comparative proteomic analysis, G. diazotrophicus proteins regulated during cocultivation were investigated. Finally, the role of some up-accumulated proteins in the response G. diazotrophicus to cocultivation was analyzed by reverse genetics, using insertion mutants. Results Our results revealed the induction of bacterial growth in response to cocultivation. Comparative proteomic analysis identified 450 bacterial proteins, with 39 up-accumulated, and 12 down-accumulated in response to cocultivation. Among the up-accumulated pathways, the metabolism of pentoses and protein synthesis were highlighted. Proteins potentially relevant to bacterial growth response such as ABC-F-Etta, ClpX, Zwf, MetE, AcnA, IlvC, and AccC were also increased. Reverse genetics analysis, using insertion mutants, revealed that the lack of ABC-F-Etta and AccC proteins severely affects G. diazotrophicus response to cocultivation. Our data demonstrated that specific mechanisms are activated in the bacterial response to plant exudates, indicating the essential role of “ribosomal activity” and “fatty acid biosynthesis” in such a process. This is the first study to demonstrate the participation of EttA and AccC proteins in plant-bacteria interactions, and open new perspectives for understanding the initial steps of such associations.

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Plant growth-promoting rhizobacteria as an alternative to mineral fertilizers in assisted bioremediation - Sustainable land and waste management

Cited by 45 publications

References 24 publications

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

ACC Deaminase Producing Bacteria With Multifarious Plant Growth Promoting Traits Alleviates Salinity Stress in French Bean (Phaseolus vulgaris) Plants

A new assay of bacterial selection with Pb reveals an unexpected effect of Pb on bacterial behavior: implications for remediation

Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus

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