Plant Growth Promoting Rhizobacteria‐Mediated Amelioration of Abiotic and Biotic Stresses for Increasing Crop Productivity

Chaudhry, Vasvi; Srivastava, Suchi; Chauhan, Puneet Singh; Singh, Poonam; Mishra, Aradhana; Nautiyal, Chandra Shekhar

doi:10.1002/9783527665334.ch6

Cited by 2 publications

(1 citation statement)

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“…Increase of proline content was observed in CMV-infected tobacco plants which may be due to disturbed electrolyte system in infected tissue and, to counter balance this, the level of proline was probably upregulated to maintaining the osmotic potential of leaf tissues. Studies have shown the role of PGPR mediated biotic stress amelioration of plants [ 54 , 55 ]. A stable and balanced intercellular redox is of vital importance to all organisms.…”

Section: Discussionmentioning

confidence: 99%

Paenibacillus lentimorbus Inoculation Enhances Tobacco Growth and Extenuates the Virulence of Cucumber mosaic virus

Kumar

Chauhan²,

Agrawal³

et al. 2016

PLoS ONE

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Previous studies with Paenibacillus lentimorbus B-30488” (hereafter referred as B-30488), a plant growth promoting rhizobacteria (PGPR) isolated from cow’s milk, revealed its capabilities to improve plant quality under normal and stress conditions. Present study investigates its potential as a biocontrol agent against an economically important virus, Cucumber mosaic virus (CMV), in Nicotiana tabacum cv. White Burley plants and delineates the physical, biophysical, biochemical and molecular perturbations due to the trilateral interactions of PGPR-host-CMV. Soil inoculation of B-30488 enhanced the plant vigor while significantly decreased the virulence and virus RNA accumulation by ~12 fold (91%) in systemic leaves of CMV infected tobacco plants as compared to the control ones. Histology of these leaves revealed the improved tissue’s health and least aging signs in B-30488 inoculated tobacco plants, with or without CMV infection, and showed lesser intercellular spaces between collenchyma cells, reduced amount of xyloglucans and pectins in connecting primary cells, and higher polyphenol accumulation in hypodermis layer extending to collenchyma cells. B-30488 inoculation has favorably maneuvered the essential biophysical (ion leakage and photosynthetic efficiency) and biochemical (sugar, proline, chlorophyll, malondialdehyde, acid phosphatase and alkaline phosphatase) attributes of tobacco plants to positively regulate and release the virus stress. Moreover, activities of defense related enzymes (ascorbate peroxidase, guaiacol peroxidase, superoxide dismutase and catalase) induced due to CMV-infection were ameliorated with inoculation of B-30488, suggesting systemic induced resistance mediated protection against CMV in tobacco. The quantitative RT-PCR analyses of the genes related to normal plant development, stress and pathogenesis also corroborate well with the biochemical data and revealed the regulation (either up or down) of these genes in favor of plant to combat the CMV mediated stress. These improvements led tobacco plant to produce more flowers and seeds with no negative impact on plant health. The present study may advocate the applicability of B-30488 for crop yield improvement in virus infested areas.

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Section: Discussionmentioning

confidence: 99%