Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi)

Patuhai, Aicah; Wahab, Puteri Edaroyati Megat; Yusoff, Martini Mohammad; Dewir, Yaser Hassan; Alsughayyir, Ali; Hakiman, Mansor

doi:10.3390/plants12162953

Cited by 1 publication

(2 citation statements)

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“…First, normal silica gel column chromatography was applied to obtain the semipure fraction from a crude plant extract. 22 In order to achieve this, different types of mobile phases were applied depending on polarity order. The purification of the semipure fraction was accomplished by using repeated silica gel column chromatography.…”

Section: Methodsmentioning

confidence: 99%

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Combined Experimental and Theoretical Insights: Spectroscopic and Molecular Investigation of Polyphenols from Fagonia indica via DFT, UV–vis, and FT-IR Approaches

Riaz,

Parveen,

Rashid

et al. 2023

ACS Omega

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This review deals with computational study of polyphenolic compounds of medicinal importance and interest for drug development. Herein, four polyphenolic compounds comprising catechol (1), caffeic acid (II), gallic acid (III), and pyrogallol (IV) have been isolated from a medicinal specie, Fagonia indica, by applying silica gel column chromatography. These compounds were identified by using gas chromatography−mass spectrometry (GC−MS) analysis and confirmed by geometric computational analysis. According to computational results, caffeic acid has shown the highest biological activation due to higher chemical softness, electronegativity (χ (eV) = −648.644), and electrostatic potential value (−8.424 × 10 −2 to +8.424 × 10 −2 ), while smaller values of chemical potential (−0.269), E LUMO (−0.080), and energy gap (ΔE = 0.149). The Mulliken atomic charges were calculated by using DFT/B3LYP with basis set 6-311G for the determination of active sites. The oxygen atom of catechol showed highest nucleophilic characteristic with a more negative charge (08 = −0.695), and pyrogallol indicated a strong electrophilic center at C14 = 0.415 with a higher positive charge. Moreover, UV−visible absorption spectra and a detailed study of vibrational frequencies for all phenolic compounds by employing the DFT approach with 3-21G, 6-31G, and 6-311G basis sets at the ground-state level showed the great agreement with experimental results. ANOVA has been applied to validate the theoretical data. Results suggest that compounds I− IV are suitable in diverse fields.

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Section: Methodsmentioning

confidence: 99%

“…indica, by applying chromatographic techniques. First, normal silica gel column chromatography was applied to obtain the semipure fraction from a crude plant extract . In order to achieve this, different types of mobile phases were applied depending on polarity order.…”

Section: Methodsmentioning

confidence: 99%