Plant Growth Regulators in Tree Rooting

Brunoni, Federica; Vielba, Jesús M.; Sánchez, Conchi

doi:10.3390/plants11060805

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Although it was proposed that these conjugates might play a role in forming of cis-OPDA active ligands, the mechanism by which OPDA-aa are formed, their role and function in plants remain elusive and poorly understood. The popularization of mass spectrometry (MS) techniques combined with the use of isotope-labeled internal standards provides the means for accurate quantification and precise identification of plant metabolites independently of the original matrix (Brunoni et al, 2022). Here, we investigated the occurrence and meaning of cis-OPDA amino acid conjugation using a liquid chromatography (LC)-MS/MS-based method to study OPDA-aa formation under different physiological conditions that alter cis-OPDA and JA homeostasis in plants.…”

Section: Discussionmentioning

confidence: 99%

Conjugation ofcis-OPDA with amino acids is a conserved pathway affectingcis-OPDA homeostasis upon stress responses

Brunoni

Široká

Mik

et al. 2023

Preprint

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Jasmonates (JAs) are a family of oxylipin phytohormones regulating plant development and growth and mediating "defense versus growth" responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) has been reported to act independently of the COI1-mediated JA signaling in several stress-induced and developmental processes. However, its means of perception and metabolism are only partially understood. Furthermore, cis-OPDA, but not JA, occurs in non-vascular plant species, such as bryophytes, exhibiting specific functions in defense and development. A few years ago, a low abundant isoleucine analog of the biologically active JA-Ile, OPDA-Ile, was detected in wounded leaves of flowering plants, opening up to the possibility that conjugation of cis-OPDA to amino acids might be a relevant mechanism for cis-OPDA regulation. Here, we extended the analysis of amino acid conjugates of cis-OPDA and identified naturally occurring OPDA-Val, OPDA-Phe, OPDA-Ala, OPDA-Glu, and OPDA-Asp in response to biotic and abiotic stress in Arabidopsis. The newly identified OPDA-amino acid conjugates show cis-OPDA-related plant responses in a JAR1-dependent manner. We also discovered that the synthesis and hydrolysis of cis-OPDA amino acid conjugates are regulated by members of the amidosynthetase GH3 and the amidohydrolase ILR1/ILL families. Finally, we found that the cis-OPDA conjugative pathway already functions in non-vascular plants and gymnosperms. Thus, one level of regulation by which plants modulate cis-OPDA homeostasis is the synthesis and hydrolysis of OPDA-amino acid conjugates, which temporarily store cis-OPDA in stress responses.

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Section: Discussionmentioning

confidence: 99%

Conjugation ofcis-OPDA with amino acids is a conserved pathway affectingcis-OPDA homeostasis upon stress responses

Brunoni

Široká

Mik

et al. 2023

Preprint

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“…Panjang akar stek rachis A. gigas sepanjang yaitu 3.9 cm akar pada konsentrasi IBA 15 mg L -1 (Tabel 5). Brunoni et al (2022) menyatakan bahwa peran utama auksin pada perbanyakan tanaman yaitu menstimulasi akar pada stek batang dan daun serta meningkatkan cabang akar. Awal terbentuknya akar dimulai dari adanya metabolisme cadangan nutrisi berupa karbohidrat yang menghasilkan energi untuk selanjutnya berperan dalam mendorong pembelahan sel dan membentuk sel-sel baru dalam jaringan.…”

Section: Panjang Akarunclassified

Penggunaan Indole Butirat Acid (IBA) untuk Induksi Akar Setek Amorphophallus titanum dan Amorphophallus gigas

Setiawan,

Yusniwati,

Handayani

et al. 2023

J Hort Indonesia

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Amorphophallus titanum dan Amorphophallus gigas merupakan flora endemik Sumatera yang terancam punah. Perbanyakan tanaman melalui setek dapat digunakan untuk menunjang kegiatan konservasi. Keberhasilan setek ditentukan oleh konsentrasi zat pengatur tumbuh (ZPT) untuk menginduksi terbentuknya akar, corm dan tunas, sehingga kajian tentang konsentrasi ZPT penting untuk dipelajari. Penelitian ini bertujuan untuk mendapatkan konsentrasi IBA terbaik untuk menginduksi akar dan corm pada A. titanum dan A. gigas. Penelitian telah dilakukan dari bulan Juli-Oktober 2022. Penelitian disusun berdasarkan rancangan acak lengkap dengan perlakuan konsentrasi IBA yang terdiri dari 5 taraf yaitu: 5, 10, 15, 20 dan 25 mg L-1. Setek pada A. titanum menggunakan rachis dan petiole, sedangkan pada A. gigas hanya menggunakan rachis. Hasil penelitian menunjukkan bahwa setek petiole menunjukkan respons yang lebih baik dibandingkan dengan rachis dalam menginduksi terbentuknya akar pada A. titanum. Konsentrasi IBA 15 mg L-1 menghasilkan persentase berakar sebesar 80% pada setek petiole A. titanum dan 20% pada setek rachis A. gigas.Kata Kunci : biodiversitas, endemik, konservasi, punah, zat pengatur tumbuh

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“…Among those factors, hormone homeostasis, and particularly auxin signaling and its crosstalk with other growth regulators, emerge as central players in the induction and development of ARs [ 5 ]. Indeed, hormones are believed to work by integrating endogenous and exogenous cues into specific gene signaling pathways that directly impact rooting processes [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Wounding-Related Signaling Is Integrated within the Auxin-Response Framework to Induce Adventitious Rooting in Chestnut

Castro-Camba,

Vielba,

Rico

et al. 2024

Genes

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Wounding and exogenous auxin are needed to induce adventitious roots in chestnut microshoots. However, the specific inductive role of wounding has not been characterized in this species. In the present work, two main goals were established: First, we prompted to optimize exogenous auxin treatments to improve the overall health status of the shoots at the end of the rooting cycle. Second, we developed a time-series transcriptomic analysis to compare gene expression in response to wounding alone and wounding plus auxin, focusing on the early events within the first days after treatments. Results suggest that the expression of many genes involved in the rooting process is under direct or indirect control of both stimuli. However, specific levels of expression of relevant genes are only attained when both treatments are applied simultaneously, leading to the successful development of roots. In this sense, we have identified four transcription factors upregulated by auxin (CsLBD16, CsERF113, Cs22D and CsIAA6), with some of them also being induced by wounding. The highest expression levels of these genes occurred when wounding and auxin treatments were applied simultaneously, correlating with the rooting response of the shoots. The results of this work clarify the genetic nature of the wounding response in chestnut, its relation to adventitious rooting, and might be helpful in the development of more specific protocols for the vegetative propagation of this species.

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Plant Growth Regulators in Tree Rooting

Abstract: Trees are long-lived organisms with complex life cycles that provide enormous benefits both in natural and cultivated stands [...]

Cited by 5 publications

References 23 publications

Conjugation ofcis-OPDA with amino acids is a conserved pathway affectingcis-OPDA homeostasis upon stress responses

Conjugation ofcis-OPDA with amino acids is a conserved pathway affectingcis-OPDA homeostasis upon stress responses

Penggunaan Indole Butirat Acid (IBA) untuk Induksi Akar Setek Amorphophallus titanum dan Amorphophallus gigas

Wounding-Related Signaling Is Integrated within the Auxin-Response Framework to Induce Adventitious Rooting in Chestnut

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