Plant hormone profiling in developing seeds of common wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.)

Matsuura, Takakazu; Mori, Izumi C.; Himi, Eiko; Hirayama, Takashi

doi:10.1270/jsbbs.19030

Cited by 20 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elute was analyzed for JA, JA-Ile, ABA, IAA, and GA 4 . The contents of these hormones were quantified using liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS) (triple quadrupole mass spectrometer with 1260 high-performance LC, G6410B; Agilent Technologies Inc., CA, USA) equipped with a ZORBAX Eclipse XDB-C18 column (Agilent Technologies Inc.) 72 .…”

Section: Methodsmentioning

confidence: 99%

Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

et al. 2022

Self Cite

View full text Add to dashboard Cite

Perception of pathogen-derived ligands by corresponding host receptors is a pivotal strategy in eukaryotic innate immunity. In plants, this is complemented by circadian anticipation of infection timing, promoting basal resistance even in the absence of pathogen threat. Here, we report that trichomes, hair-like structures on the epidermis, directly sense external mechanical forces, including raindrops, to anticipate pathogen infections in Arabidopsis thaliana. Exposure of leaf surfaces to mechanical stimuli initiates the concentric propagation of intercellular calcium waves away from trichomes to induce defence-related genes. Propagating calcium waves enable effective immunity against pathogenic microbes through the CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR 3 (CAMTA3) and mitogen-activated protein kinases. We propose an early layer of plant immunity in which trichomes function as mechanosensory cells that detect potential risks.

show abstract

Section: Methodsmentioning

confidence: 99%

Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Phytohormone content (abscisic acid (ABA); indoleacetic acid (IAA); isopentenyladenine (iP); trans-zeatin (tZ); jasmonic acid (JA); jasmonoyl-isoleucine (JA-Ile); gibberellin A 1 , (GA 1 ); gibberellin A 4 , GA 4 ; and salicylic acid, SA) was determined by previously reported method [ 41 ] with specified modifications. In brief, dry pericarps were homogenized to a fine powder and 50 mg of the sample was suspended in 4 mL of the extraction buffer (1% ( v/v ) acetic acid in acetonitrile/water (4:1)) with a mixture of stable isotope-labeled internal standards (IS) [ 41 ]. Suspended samples were extracted for 1 h at 4 °C and centrifuged at 3000× g for 10 min at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity

Swetha

Singiri

Novoplansky

et al. 2021

Plants

View full text Add to dashboard Cite

In the face of climate change and the predicted increase in the frequency and severity of abiotic stresses (e.g., hot spell, salinity), we sought to investigate the effect of salinity (S), short episodes of high temperature (HS) and combination of salinity and high temperature (SHS), at the reproductive phase, on yield with a special focus on the properties of dead pericarps of Brassica juncea. Three interval exposures to HS resulted in massive seed abortion, and seeds from salt-treated plants germinated poorly. Germination rate and final germination of B. juncea seeds were slightly reduced in the presence of salt and SHS pericarp extracts. All pericarp extracts completely inhibited seed germination of tomato and Arabidopsis, but removal of pericarp extracts almost fully restored seed germination. Heat and salinity profoundly affected the accumulation of phytohormones in dead pericarps. Combined stresses highly reduced IAA and ABA levels compared with salt, and enhanced the accumulation of GA1, but abolished the positive effect of salt on the accumulation of GA4, JA and SA. Interestingly, pericarp extracts displayed priming activity and significantly affected seedling performance in a manner dependent on the species and on the origin of the pericarp. While control pericarps improved and reduced the seedlings’ performance of autologous and heterologous species, respectively, pericarps from salt-treated plants were harmless or improved heterologous seedling performance. Thus, the strategy employed by the germinating seed for securing resources is set up, at least partly, by the mother plant in conjunction with the maternal environment whose components are stored in the dead maternal organs enclosing the embryo.

show abstract

“…Endogenous ABA in whole roots was quantified by LC‐MS as previously described by Matsuura et al . (2019) (see Methods S5).…”

Section: Methodsmentioning

confidence: 99%

Abscisic acid is required for exodermal suberization to form a barrier to radial oxygen loss in the adventitious roots of rice (Oryza sativa)

et al. 2021

Self Cite

View full text Add to dashboard Cite

Summary To acclimate to waterlogged conditions, wetland plants form a barrier to radial oxygen loss (ROL) that can enhance oxygen transport to the root apex. We hypothesized that one or more hormones are involved in the induction of the barrier and searched for such hormones in rice. We previously identified 98 genes that were tissue‐specifically upregulated during ROL barrier formation in rice. The RiceXPro database showed that most of these genes were highly enhanced by exogenous abscisic acid (ABA). We then examined the effect of ABA on ROL barrier formation by using an ABA biosynthesis inhibitor (fluridone, FLU), by applying exogenous ABA and by examining a mutant with a defective ABA biosynthesis gene (osaba1). FLU suppressed barrier formation in a stagnant solution that mimics waterlogged soil. Under aerobic conditions, rice does not naturally form a barrier, but 24 h of ABA treatment induced barrier formation. osaba1 did not form a barrier under stagnant conditions, but the application of ABA rescued the barrier. In parallel with ROL barrier formation, suberin lamellae formed in the exodermis. These findings strongly suggest that ABA is an inducer of suberin lamellae formation in the exodermis, resulting in an ROL barrier formation in rice.

show abstract

Plant hormone profiling in developing seeds of common wheat (<i>Triticum aestivum</i> L.)

Cited by 20 publications

References 45 publications

Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Mechanosensory trichome cells evoke a mechanical stimuli–induced immune response in Arabidopsis thaliana

Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity

Abscisic acid is required for exodermal suberization to form a barrier to radial oxygen loss in the adventitious roots of rice (Oryza sativa)

Contact Info

Product

Resources

About