Plant hormones in defense response of Brassica napus to Sclerotinia sclerotiorum – Reassessing the role of salicylic acid in the interaction with a necrotroph

Nováková, Miroslava; Šašek, Vladimír; Dobrev, Petre I.; Valentová, Olga; Burketová, Lenka

doi:10.1016/j.plaphy.2014.04.019

Cited by 89 publications

(61 citation statements)

References 48 publications

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“…napus that were sensitive or resistant to S . sclerotiorum [46, 47]. Our results differ with those studies regarding time, as our results demonstrated that SA signaling was activated later, at 6–10 dpi, while those studies confirmed that SA signaling was activated earlier, at 12 h [46] or 48 h post infection [47].…”

Section: Discussioncontrasting

confidence: 99%

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

et al. 2017

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Plants have the ability to resist pathogen attack after infection or treatment with biotic and abiotic elicitors. In oilseed rape plant Brassica napus AACC and in the artificially synthesized Raphanus alboglabra RRCC, the root-colonizing Trichoderma harzianum TH12 fungus triggers induced systemic resistance (ISR), and its culture filtrate (CF) triggers a systemic acquired resistance (SAR) response against infection by the Sclerotinia sclerotiorum. Salicylic acid (SA) and jasmonic acid/ethylene (JA/ET) are plant hormone signals that play important roles in the regulation of ISR and SAR. In this study, at six different time points (1, 2, 4, 6, 8 and 10 days post-infection [dpi]), six resistance genes were used as markers of signaling pathways: JA/ET signaling used AOC3, PDF1.2 and ERF2 genes, while PR-1, TGA5 and TGA6 genes were used as markers of SA signaling. The results of quantitative real-time polymerase chain reaction (qRT-PCR) showed that AOC3, PDF1.2 and ERF2 expression levels in infected leaves of AACC and RRCC increase at 1 and 2 dpi with S. sclerotiorum or inoculation with TH12. PR-1, TGA5 and TGA6 expression levels increased at 8 and 10 dpi in infected leaves. PR-1, TGA5 and TGA6 expression levels increased early in plants treated with CF in both of the healthy genotypes. Furthermore, induction of SA- and JA/ET-dependent defense decreased disease symptoms in infected leaves at different times. The results suggest that the RRCC genotype exhibits resistance to disease and that the ability of TH12 and its CF to induce systemic resistance in susceptible and resistant oilseed rape genotypes exists. In addition, the results indicate for the first time that in RRCC the SA signaling pathway is involved in resistance to necrotrophic pathogens.

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Section: Discussioncontrasting

confidence: 99%

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

et al. 2017

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“…Although S. sclerotiorum is a necrotrophic fungal pathogen, at the early stages of infection it grows in the apoplast without crossing the plant cell wall and the host cells are still alive at that stage (Kabbage et al ., ). The defense against S. sclerotiorum in Arabidopsis and oilseed rape is also associated with SA signaling (Guo & Stotz, ; Wang et al ., ; Novakova et al ., ). Furthermore, we found that AtPR1 ‐overexpressing transgenic Arabidopsis showed more resistance to S. sclerotiorum (Fig.…”

Section: Discussionmentioning

confidence: 97%

A cerato‐platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum

et al. 2017

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Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense .

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“…The early-discovered phytohormones auxin, gibberellin (GA), cytokinin, abscisic acid (ABA) and ethylene are generally referred to as the five 'classic' plant hormones, whereas brassinosteroid (BR), jasmonate (JA), salicylate (SA), strigolactone (SL), nitric oxide (NO), polyamine, and peptide are recognized as new families of phytohormones. These compounds are important regulators that mediate plant growth, differentiation, development and responses to both biotic and abiotic stresses by means of synergistic or antagonistic actions [2]. Many studies have revealed the role of each class of phytohormone, and in recent years, molecular genetic studies have been elucidating complicated crosstalk among phytohormones [3,4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

Cui

Lin

Zhou

et al. 2015

Microchemical Journal

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Plant hormones in defense response of Brassica napus to Sclerotinia sclerotiorum – Reassessing the role of salicylic acid in the interaction with a necrotroph

Cited by 89 publications

References 48 publications

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

Detecting the Hormonal Pathways in Oilseed Rape behind Induced Systemic Resistance by Trichoderma harzianum TH12 to Sclerotinia sclerotiorum

A cerato‐platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum

Comparison of sample pretreatment methods for the determination of multiple phytohormones in plant samples by liquid chromatography–electrospray ionization-tandem mass spectrometry

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