Plant immune system activation is necessary for efficient root colonization by auxin-secreting beneficial bacteria

Tzipilevich, Elhanan; Russ, Dor; Dangl, Jeffery L.; Benfey, Philip N.

doi:10.1016/j.chom.2021.09.005

Cited by 104 publications

(59 citation statements)

References 81 publications

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“…Bacterial auxin is essential for root colonization by B. velezensis and for its survival and replication by antagonizing ROS produced as part of the receptor EFR-triggered immune response. Meanwhile, ROS induces the synthesis of bacterial auxin (Tzipilevich et al, 2021).…”

Section: Soil Is a Critical Factor Affecting The Root Microbiotamentioning

confidence: 99%

The root microbiome: Community assembly and its contributions to plant fitness

Bai

Liu

Qiu

et al. 2022

JIPB

202

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Section: Soil Is a Critical Factor Affecting The Root Microbiotamentioning

confidence: 99%

The root microbiome: Community assembly and its contributions to plant fitness

Bai

Liu

Qiu

et al. 2022

JIPB

202

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“…In addition, the first defence line of plants to repel intruded microbes is microbe‐associated molecular patterns (MAMPs)‐triggered immunity. ROS burst is involved in MAMP‐triggered immunity by directly inhibiting microbe growth and acting signal molecule to induce immune‐related genes (Tzipilevich et al ., 2021). Previous studies have indicated that fungal antioxidative system, such as glutathione‐S‐transferase (GST) is induced during infection to detoxify ROS (Gullner et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Priming of rhizobial nodulation signaling in the mycosphere accelerates nodulation of legume hosts

et al. 2022

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The simultaneous symbiosis of leguminous plants with two root mutualists, endophytic fungi and rhizobia is common in nature, yet how two mutualists interact and co-exist before infecting plants and the concomitant effects on nodulation are less understood.Using a combination of metabolic analysis, fungal deletion mutants and comparative transcriptomics, we demonstrated that Bradyrhizobium and a facultatively biotrophic fungus, Phomopsis liquidambaris, interacted to stimulate fungal flavonoid production, and thereby primed Bradyrhizobial nodulation signaling, enhancing Bradyrhizobial responses to root exudates and leading to early nodulation of peanut (Arachis hypogaea), and such effects were compromised when disturbing fungal flavonoid biosynthesis.Stress sensitivity assays and reactive oxygen species (ROS) determination revealed that flavonoid production acted as a strategy to alleviate hyphal oxidative stress during P. liquidambaris-Bradyrhizobial interactions. By investigating the interactions between P. liquidambaris and a collection of 38 rhizobacteria, from distinct bacterial genera, we additionally showed that the flavonoid-ROS module contributed to the maintenance of fungal and bacterial co-existence, and fungal niche colonization under soil conditions.Our results demonstrate for the first time that rhizobial nodulation signaling can be primed by fungi before symbiosis with host plants and highlight the importance of flavonoid in tripartite interactions between legumes, beneficial fungi and rhizobia.

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“…It should be noted that although we detected endogenous IAA production in strain K ( Fan and Smith, 2021 ), there is uncertainty regarding the importance of this mechanism. We cannot conclude that bacterial auxin secreted by strain K plays a key role in plant fitness unless the mutant bacterial strain has been tested ( Tzipilevich et al, 2021 ). Strain K also ameliorated salinity stress in regard to root dry weight and root length ( Figures 3B , C ).…”

Section: Discussionmentioning

confidence: 99%

Mucilaginibacter sp. K Improves Growth and Induces Salt Tolerance in Nonhost Plants via Multilevel Mechanisms

Fan

Smith

2022

Front. Plant Sci.

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Soil salinity negatively modulates plant growth and development, contributing to severe decreases in the growth and production of crops. Mucilaginibacter sp. K is a root endophytic bacterium that was previously reported by our laboratory to stimulate growth and confer salt tolerance in Arabidopsis (Arabidopsis thaliana). The main purpose of the present study is to elucidate the physiological and molecular machinery responsible for the prospective salt tolerance as imparted by Mucilaginibacter sp. K. We first report that auxin, gibberellin, and MPK6 signalings were required for strain K-induced growth promotion and salt tolerance in Arabidopsis. Then, this strain was assessed as a remediation strategy to improve maize performance under salinity stress. Under normal growth conditions, the seed vigor index, nitrogen content, and plant growth were significantly improved in maize. After NaCl exposure, strain K significantly promoted the growth of maize seedlings, ameliorated decline in chlorophyll content and reduced accretion of MDA and ROS compared with the control. The possible mechanisms involved in salt resistance in maize could be the improved activities of SOD and POD (antioxidative system) and SPS (sucrose biosynthesis), upregulated content of total soluble sugar and ABA, and reduced Na+ accumulation. These physiological changes were then confirmed by induced gene expression for ion transportation, photosynthesis, ABA biosynthesis, and carbon metabolism. In summary, these results suggest that strain K promotes plant growth through increases in photosynthesis and auxin- and MPK6-dependent pathways; it also bestows salt resistance on plants through protection against oxidative toxicity, Na+ imbalance, and osmotic stress, along with the activation of auxin-, gibberellin-, and MPK6-dependent signaling pathways. This is the first detailed report of maize growth promotion by a Mucilaginibacter sp. strain from wild plant. This strain could be used as a favorable biofertilizer and a salinity stress alleviator for maize, with further ascertainment as to its reliability of performance under field conditions and in the presence of salt stress.

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Plant immune system activation is necessary for efficient root colonization by auxin-secreting beneficial bacteria

Cited by 104 publications

References 81 publications

The root microbiome: Community assembly and its contributions to plant fitness

The root microbiome: Community assembly and its contributions to plant fitness

Priming of rhizobial nodulation signaling in the mycosphere accelerates nodulation of legume hosts

Mucilaginibacter sp. K Improves Growth and Induces Salt Tolerance in Nonhost Plants via Multilevel Mechanisms

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