Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants

Mahna, Nasser; Vahed, Sepideh Zununi; Khani, Sajjad

doi:10.4172/2157-7439.1000161

Cited by 45 publications

(26 citation statements)

References 26 publications

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“…), Arabidopsis (Arabidopsis thaliana), cucurbits, and others. It is suggested that proper use of low-dose Agnanoparticles may even stimulate growth and survival of seedlings or plant juvenile phases, exhibiting concurrently a sterilizing effect against pathogenic microorganisms from their environment to protect plants against diseases in their early stages of life [26,27].…”

Section: Resultsmentioning

confidence: 99%

Biocidal Properties of Silver-Nanoparticles in Water Environments

Kowalska‐Góralska¹,

Senze²,

Polechoński³

et al. 2015

Pol. J. Environ. Stud.

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IntroductionNanotechnology enables manufacturing of materials in atom scale (in the range from 1 to 100 nm). Their primary use is for electronics and electrical engineering, but more often they are finding applications in pharmacy, cosmetology, and medical techniques. They are characterized by a number of unusual properties such as: mechanical (strengthening other materials), electrical (emission and conductivity), magnetic, and biological. Nanoparticle applications are currently so common that consumers are often not aware that they are using them. The variety of Pol. J. Environ. Stud. Vol. 24, No. 4 (2015), [1641][1642][1643][1644][1645][1646][1647] AbstractNanotechnology offers manufacturing and use of structures in which at least one dimension is expressed in nanometers. An increasing number of everyday products contain some ingredients in molecules size. One of them -Ag-nanoparticles (nanosilver) -is commonly used due to its biocidal properties. There is a serious risk of nanoparticles being released into the environment, especially to the soil or surface water. Because of low selectivity of silver-nanoparticles in water it is not an environmentally-friendly product (destroying aquatic microflora and fauna).The aim of this study was to determine the effect of Ag-nanoparticles on the growth and survivability of three water plants: Oedogonium sp., Versicularia dubyana, and Lagarosyphon madagascariensis.Plants were treated with silver nanoparticle solutions of concentrations ranging from 0.0 to 10.0 mg·dm -3 Ag (NANO SILVER product by NANOCO Corp.). Light conditions were controlled through 16-hour light cycle using a 15W Tungsram lamp. The whole experiment was carried out over 10 days. Changes in plant morphology and growth in various Ag-nanoparticle concentrations were observed.Ag-nanoparticles showed biocidal activity for Oedogonium sp. after 24 hours of incubation in solutions with a concentration of 1.0-10.0 mg·dm -3 Ag. Plants were dark, destroyed, and sank to the bottom. In samples with higher plants, nano silver caused blackening and some plant deaths at concentrations of 5.0 and 10.0 mg·dm -3 Ag, or inhibited their growth in lower concentrations. Selective activity of nano silver may be useful for eliminating unwanted plants. However, its uncontrolled release into the environment may be harmful to aquatic organisms and devastating for entire ecosystems.

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Section: Resultsmentioning

confidence: 99%

Biocidal Properties of Silver-Nanoparticles in Water Environments

Kowalska‐Góralska¹,

Senze²,

Polechoński³

et al. 2015

Pol. J. Environ. Stud.

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“…27 Leaf explants of Vitis vinifera 'Farkhi', 'Khoshnave' and 'Rashe' were successfully decontaminated with 95% EtOH for 30 s, and 1000 mg L À1 Ag NPs for 20 min. 28 Mahna et al 29 investigated the effect of Ag NP treatment on surface disinfection of Arabidopsis seeds, potato leaves and tomato cotyledons. The treatment of the explants with 100 mg L À1 Ag NPs for 1 or 5 min was found to be ideal for decontaminating (100%) the seeds, leaves or cotyledons and this treatment had no side effects on explant viability.…”

Section: Input Of Nanomaterials For Surface Disinfection Of Explantsmentioning

confidence: 99%

Nanomaterials in plant tissue culture: the disclosed and undisclosed

2017

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“…Ag NP has successfully controlled bacterial and fungal infection without any adverse effect on regeneration of lemongrass plantations (Fakhrfeshani et al, 2012). It has also been reported that NS can be an effective tool for eliminating contaminants from tissues only if the correct dose and exposure time are used (Mahna et al, 2013). However, NS has not yet become a universal decontaminant and our result will contribute to future research to find an effective way to control microbial contamination in the plant tissue culture.…”

Section: Resultsmentioning

confidence: 69%

Effect of Agno3 Nanoparticles on Sterilization and Alkaloid Production of Catherenthus Roseus Tissue By

Shahin

2018

Al-Azhar Journal of Pharmaceutical Sciences

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Bacterial contamination is a serious problem in plant tissue culture procedures. An experiment has been conducted to evaluate the potential of nanosilver (NS) to remove bacterial contaminants from Catherenthus shoot tips and single nodes. The experiments were performed as a totally randomized design in a faculty with four replications and each replicate with ten explants. Treatments involved NS in two steps (before and after surface sterilization along with control) at three concentrations (25, 50 and 100 mg 1-1) at three times (30, 45 and 60 minutes). Plants were grown on MS medium supplemented with 5 mg l-1 of Kinetin (KIN) and 0.1 mgl-1 of naphthaline acetic acid (NAA). The results showed that the use of 100 mg l-1 NS solution after sterilization of the surface resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the measured characters. According to the data obtained in the experience, it was concluded that NS had a good view to eliminate bacterial diseases in plant tissue culture procedures. Further research on other cultural species requires that in the tissue cultures of other cultures, to enhance the effectiveness of the NS in order to eliminate bacterial diseases.

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Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants

Cited by 45 publications

References 26 publications

Biocidal Properties of Silver-Nanoparticles in Water Environments

Biocidal Properties of Silver-Nanoparticles in Water Environments

Nanomaterials in plant tissue culture: the disclosed and undisclosed

Effect of Agno3 Nanoparticles on Sterilization and Alkaloid Production of Catherenthus Roseus Tissue By

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