Plant lectins: the ties that bind in root symbiosis and plant defense

Hoff, Peter L. De; Brill, Laurence M.; Hirsch, Ann M.

doi:10.1007/s00438-009-0460-8

Cited by 200 publications

(143 citation statements)

References 112 publications

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…In a bobtail squid and a lucinid clam, the time frame for symbiont infection can be significantly extended by experimental elimination of the symbiont inoculum in the ambient seawater (19,36), which might represent a fail-safe mechanism to ensure the symbiotic associations. In squid-Vibrio, legume-Rhizobium, and coral-Symbiodinium associations, sugar-sugar and/or sugar-lectin interactions have been identified to mediate the initial contact between the host and the symbiont cell surfaces (9,20,41,52). Such mechanisms involved in the infection competence and localization during the development of the midgut crypts in early instars of R. pedestris (Fig.…”

Section: Discussionmentioning

confidence: 99%

Specific Developmental Window for Establishment of an Insect-Microbe Gut Symbiosis

Kikuchi

Hosokawa

Fukatsu

2011

Appl Environ Microbiol

119

144

View full text Add to dashboard Cite

The alydid stinkbug Riptortus pedestris is specifically associated with a beneficial Burkholderia symbiont in the midgut crypts. Exceptional among insect-microbe mutualistic associations, the Burkholderia symbiont is not vertically transmitted but orally acquired by nymphal insects from the environment every generation. Here we experimentally investigated the process of symbiont acquisition during the nymphal development of R. pedestris. In a field population, many 2nd instar nymphs were Burkholderia free, while all 3rd, 4th, and 5th instar nymphs were infected. When reared on soil-grown potted soybean plants, Burkholderia acquisition occurred at a drastically higher frequency in the 2nd instar than in the other instars. Oral administration of cultured Burkholderia cells showed that 2nd and 3rd instar nymphs are significantly more susceptible to the symbiont infection than 1st, 4th, and 5th instar nymphs. Histological observations revealed rudimentary midgut crypts in the 1st instar, in contrast to well-developed midgut crypts in the 2nd and later instars. These results indicate that R. pedestris acquires the Burkholderia symbiont from the environment mainly during the 2nd instar period and strongly suggest that the competence for the symbiont infection is developmentally regulated by the host side. Potential mechanisms involved in infection competence and possible reasons why the infection preferentially occurs in the 2nd instar are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Specific Developmental Window for Establishment of an Insect-Microbe Gut Symbiosis

Kikuchi

Hosokawa

Fukatsu

2011

Appl Environ Microbiol

119

144

View full text Add to dashboard Cite

show abstract

“…In analogy to animal cells (41), tethering into larger complexes (lipid rafts) through interaction with endogenous lectins seems to occur in plant cells, e.g. at the root surface, probably mediating symbiosis with mutualistic bacteria and/or defense of pathogenic bacteria (42). We recently published for Arabidopsis receptor-like kinase EFR that mutation of a single conserved N-glycosylation site, in addition to enhancing accumulation in the ER, abolished elf-ligand binding at the plasma membrane (2).…”

Section: Discussionmentioning

confidence: 99%

Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes

Kaulfürst-Soboll

Rips

Koiwa

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

Arabidopsis N-glycosylation mutants with enhanced salt sensitivity show reduced immunoreactivity of complex N-glycans. Among them, hybrid glycosylation 1 (hgl1) alleles lacking Golgi ␣-mannosidase II are unique, because their glycoprotein N-glycans are hardly labeled by anti-complex glycan antibodies, even though they carry ␤1,2-xylose and ␣1,3-fucose epitopes. To dissect the contribution of xylose and core fucose residues to plant stress responses and immunogenic potential, we prepared Arabidopsis hgl1 xylT double and hgl1 fucTa fucTb triple mutants by crossing previously established T-DNA insertion lines and verified them by mass spectrometry analyses. Root growth assays revealed that hgl1 fucTa fucTb but not hgl1 xylT plants are more salt-sensitive than hgl1, hinting at the importance of core fucose modification and masking of xylose residues. Detailed immunoblot analyses with anti-␤1,2-xylose and anti-␣1,3-fucose rabbit immunoglobulin G antibodies as well as cross-reactive carbohydrate determinant-specific human immunoglobulin E antibodies (present in sera of allergy patients) showed that xylose-specific reactivity of hgl1 N-glycans is indeed reduced. Based on three-dimensional modeling of plant N-glycans, we propose that xylose residues are tilted by 30°b ecause of untrimmed mannoses in hgl1 mutants. Glycosidase treatments of protein extracts restored immunoreactivity of hgl1 N-glycans supporting these models. Furthermore, among allergy patient sera, untrimmed mannoses persisting on the ␣1,6-arm of hgl1 N-glycans were inhibitory to immunoreaction with core fucoses to various degrees. In summary, incompletely trimmed glycoprotein N-glycans conformationally prevent xylose and, to lesser extent, core fucose accessibility.

show abstract

“…Proteins secreted by roots are important for recognition of pathogenic and non-pathogenic bacteria [221,222]. For example, lectins function as defense factors and recognition in symbiotic interactions [223]. During the flowering period of Arabidopsis thaliana, roots excrete more protein involved in defense, such as chitinases, glucanases and myrosinases [224].…”

Section: Root Exudation the Ecological Driver Of Microbial Communitimentioning

confidence: 99%

Root Exudation: The Ecological Driver of Hydrocarbon Rhizoremediation

2016

View full text Add to dashboard Cite

Rhizoremediation is a bioremediation technique whereby microbial degradation of organic contaminants occurs in the rhizosphere. It is considered to be an effective and affordable "green technology" for remediating soils contaminated with petroleum hydrocarbons. Root exudation of a wide variety of compounds (organic, amino and fatty acids, carbohydrates, vitamins, nucleotides, phenolic compounds, polysaccharides and proteins) provide better nutrient uptake for the rhizosphere microbiome. It is thought to be one of the predominant drivers of microbial communities in the rhizosphere and is therefore a potential key factor behind enhanced hydrocarbon biodegradation. Many of the genes responsible for bacterial adaptation in contaminated soil and the plant rhizosphere are carried by conjugative plasmids and transferred among bacteria. Because root exudates can stimulate gene transfer, conjugation in the rhizosphere is higher than in bulk soil. A better understanding of these phenomena could thus inform the development of techniques to manipulate the rhizosphere microbiome in ways that improve hydrocarbon bioremediation.

show abstract

Plant lectins: the ties that bind in root symbiosis and plant defense

Cited by 200 publications

References 112 publications

Specific Developmental Window for Establishment of an Insect-Microbe Gut Symbiosis

Specific Developmental Window for Establishment of an Insect-Microbe Gut Symbiosis

Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes

Root Exudation: The Ecological Driver of Hydrocarbon Rhizoremediation

Contact Info

Product

Resources

About