Plant lignan secoisolariciresinol suppresses pericardial edema caused by dioxin-like compounds in developing zebrafish: Implications for suppression of morphological abnormalities

Tokunaga, Saimi; Woodin, Bruce R.; Stegeman, John J.

doi:10.1016/j.fct.2016.07.012

Cited by 13 publications

(6 citation statements)

References 56 publications

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“…The observed deformities were: pericardium oedema, oedema of the yolk sac and a spine deformity showns in figure 5. Such deformities were observed by several authors who studied the toxicity of drugs and some metals: Akande et al (2010); Li et al (2017); Oliveira et al (2009); Oliveira et al (2013); Tesolin et al (2014); Tokunaga et al (2016). All deformations were statistically different from the controls.…”

Section: Short-term Chronic Ecotoxicity Test With Fish Larvaementioning

confidence: 69%

Evaluation of resveratrol toxicity in the embryolarval stage of Danio rerio fish

Cavalcante¹,

Lopes-Ferreira²,

Rogero³

et al. 2024

Ecotoxicol. Environ. Contam.

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Human beings concern about healthy life has driven researchers to study new compounds capable of reaching that desire. Resveratrol (3, 4 ', 5-trihydroxystilbene) a phenolic compound, is one of these substances which presents a variety of pharmacological actions, as antioxidant potential, anti-inflammatory capacity, protection against heart and cancer diseases. Despite the resveratrol health benefits studies, there is a little evidence of its toxicity in the literature in aquatic organisms, and especially the data on the concentration of resveratrol in the environment, making the present study fundamental for information about resveratrol ecotoxicity in the aquatic system. The aim of this study was to evaluate the toxicity of resveratrol in embryos and larvae of Danio rerio (zebrafish). The in vitro cytotoxicity and ecotoxicity assays were performed. The IC 50 obtained in the NCTC-L929 cell line cytotoxicity assay was 38.5 mg L-1. The LC 50 (96h) obtained in fish embryo toxicity test was 75.3 mg L-1 and the mean value of resveratrol LC 50 (168h) obtained in the short-term chronic ecotoxicity assays performed with zebrafish larvae was 51.4 mg L-1. This work provided data on the toxicity of resveratrol in the embryonic stage of fish of the species Danio rerio and the toxic effects are dependent on its concentration.

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Section: Short-term Chronic Ecotoxicity Test With Fish Larvaementioning

confidence: 69%

Evaluation of resveratrol toxicity in the embryolarval stage of Danio rerio fish

Cavalcante¹,

Lopes-Ferreira²,

Rogero³

et al. 2024

Ecotoxicol. Environ. Contam.

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“…PE was a sign of cardiac dysplasia; most chemicals with cardiotoxic effects would induce such abnormal phenotypes in zebrafish. 33 Exposure of 27-DBCZ at EC 50 exerted a significant appearance of PE in zebrafish larvae, and this phenotype started to develop around 72 hpf and increased in severity as development progressed. Highly consistent phenotypic alterations were found in larvae exposed to other polyhalogenated carbazoles (PHCZs), including 3-BCZ, 36-DBCZ, and 1368-TBCZ at each corresponding EC 50 concentration.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

Deep Learning-Enabled Morphometric Analysis for Toxicity Screening Using Zebrafish Larvae

Dong

Wang

et al. 2023

Environ. Sci. Technol.

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Toxicology studies heavily rely on morphometric analysis to detect abnormalities and diagnose disease processes. The emergence of ever-increasing varieties of environmental pollutants makes it difficult to perform timely assessments, especially using in vivo models. Herein, we propose a deep learning-based morphometric analysis (DLMA) to quantitatively identify eight abnormal phenotypes (head hemorrhage, jaw malformation, uninflated swim bladder, pericardial edema, yolk edema, bent spine, dead, unhatched) and eight vital organ features (eye, head, jaw, heart, yolk, swim bladder, body length, and curvature) of zebrafish larvae. A data set composed of 2532 bright-field micrographs of zebrafish larvae at 120 h post fertilization was generated from toxicity screening of three categories of chemicals, i.e., endocrine disruptors (perfluorooctanesulfonate and bisphenol A), heavy metals (CdCl 2 and PbI 2 ), and emerging organic pollutants (acetaminophen, 2,7-dibromocarbazole, 3-monobromocarbazo, 3,6-dibromocarbazole, and 1,3,6,8-tetrabromocarbazo). Two typical deep learning models, one-stage and two-stage models (TensorMask, Mask R-CNN), were trained to implement phenotypic feature classification and segmentation. The accuracy was statistically validated with a mean average precision >0.93 in unlabeled data sets and a mean accuracy >0.86 in previously published data sets. Such a method effectively enables subjective morphometric analysis of zebrafish larvae to achieve efficient hazard identification of both chemicals and environmental pollutants.

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“…It has been reported that difenoconazole can cause a lot of symptoms during the development of zebra sh embryos, such as slow heart rate, morphological abnormality and hatching inhibition (Mu et al, 2013). Dioxin and dioxin-like compounds can cause morphological abnormalities of zebra sh embryos, including pericardial edema and craniofacial abnormalities (Tokunaga et al, 2016).It was found that the heart function of zebra sh embryos was impaired, the heart rate decreased obviously, and serious changes such as pericardial edema, yolk sac edema and yolk sac deformation occurred during the time when zebra sh embryos were exposed to cyhalofop-butyl and quizalofop-p-ethyl, and the co-exposure aggravated these changes. In addition, abnormalities mainly occur in cardiac areas, such as pericardial edema.…”

Section: Discussionmentioning

confidence: 99%

Combined Developmental Toxicity Of Cyhalofop-butyl And Quizalofop-p-ethyl On The Zebrafish (Danio rerio) Embryos

Liu

Wang²,

Li³

et al. 2022

Preprint

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Aryloxyphenoxypropionate herbicides have the characteristics of high efficiency, low toxicity, and safety to subsequent crops, and occupy an important position in the world herbicide market. Cyhalofop-butyl and quizalofop-p-ethyl are two representative herbicides, which are widely used in weed control. However, there is limited information on their combined toxicity to aquatic organisms. In this study, the developmental toxicity of cyhalofop-butyl and quizalofop-p-ethyl exposure in combination on zebrafish embryos was valuated to better understand the interaction between the that. The 96 h-LC50 (50% lethal concentration) of cyhalofop-butyl and quizalofop-p-ethyl on zebrafish embryos were 0.637 mg·L− 1 and 0.248 mg·L− 1, respectively. The combined effect of cyhalofop-butyl and quizalofop-p-ethyl was an antagonistic effect, and the 96 h-LC50 of zebrafish embryos was 1.043 mg·L− 1. Morphologically distinct pericardial edema and yolk cysts were observed after combined exposure, with significant effects on body length and heart rate in zebrafish embryos. At the same time, the mRNA levels of gene related to apoptosis and cardiac development also changed significantly. Therefore, we speculate that changes in genes related to apoptosis and cardiac development should be responsible for the abnormal development during embryonic development following co-exposure of cyhalofop-butyl and quizalofop-p-ethyl.

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Plant lignan secoisolariciresinol suppresses pericardial edema caused by dioxin-like compounds in developing zebrafish: Implications for suppression of morphological abnormalities

Cited by 13 publications

References 56 publications

Evaluation of resveratrol toxicity in the embryolarval stage of Danio rerio fish

Evaluation of resveratrol toxicity in the embryolarval stage of Danio rerio fish

Deep Learning-Enabled Morphometric Analysis for Toxicity Screening Using Zebrafish Larvae

Combined Developmental Toxicity Of Cyhalofop-butyl And Quizalofop-p-ethyl On The Zebrafish (Danio rerio) Embryos

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