Plant Materials Use in the Production of Fermented Milk Products for Specialized Nutrition
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Cited by 3 publications
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Abiotic and biotic stresses have a major impact on crop growth. Stress affects the root system and decreases the amount of nutrients in fruits. Modern agricultural technologies help replace mineral fertilizers with new generation biopreparation. Unlike chemical fertilizers, biofertilizers reduce the risk of adverse environmental impacts. Of special interest are extremophilic microorganisms able to survive in extreme conditions. We aimed to study the phytostimulating ability of extremophilic bacteria isolated from disturbed lands in the coal-mining region. We isolated microorganisms from disturbed lands and studied their cultural, morphological, and biochemical properties. Then, we determined their ability to synthesize indole-3-acetic acids. The extremophilic bacteria were identified and subjected to biocompatibility testing by co-cultivation. Next, we created consortia of pure cultures and analyzed biomass growth. Finally, the biopreparation was experimentally tested on Trifolium prantense L. seeds. We isolated 10 strains of microorganisms that synthesized 4.39 to 16.32 mg/mL of indole-3-acetic acid. The largest amounts of the acid were produced by Pantoea spp., Enterococcus faecium, Leclercia spp., Rothia endophytica, and Klebsiella oxytoca. A consortium of Pantoea spp., E. faecium, and R. endophytica at a ratio of 1:1:1 produced the largest amount of indole-3-acetic acid (15.59 mg/mL) and accumulated maximum biomass. The addition of 0.2% L-tryptophan to the nutrient medium increased the amount of indole-3-acetic acid to 18.45 mg/mL. When the T. prantense L. seeds were soaked in the biopreparation (consortium’s culture fluid) at a concentration of 2.5, the sprouts were 1.4 times longer on the 10th day of growth, compared to the control. The consortium of Pantoea spp., E. faecium, and R. endophytica (1:1:1) stimulated the growth of T. prantense L. seeds. Our findings can be further used to develop biofertilizers for agriculture.
Abiotic and biotic stresses have a major impact on crop growth. Stress affects the root system and decreases the amount of nutrients in fruits. Modern agricultural technologies help replace mineral fertilizers with new generation biopreparation. Unlike chemical fertilizers, biofertilizers reduce the risk of adverse environmental impacts. Of special interest are extremophilic microorganisms able to survive in extreme conditions. We aimed to study the phytostimulating ability of extremophilic bacteria isolated from disturbed lands in the coal-mining region. We isolated microorganisms from disturbed lands and studied their cultural, morphological, and biochemical properties. Then, we determined their ability to synthesize indole-3-acetic acids. The extremophilic bacteria were identified and subjected to biocompatibility testing by co-cultivation. Next, we created consortia of pure cultures and analyzed biomass growth. Finally, the biopreparation was experimentally tested on Trifolium prantense L. seeds. We isolated 10 strains of microorganisms that synthesized 4.39 to 16.32 mg/mL of indole-3-acetic acid. The largest amounts of the acid were produced by Pantoea spp., Enterococcus faecium, Leclercia spp., Rothia endophytica, and Klebsiella oxytoca. A consortium of Pantoea spp., E. faecium, and R. endophytica at a ratio of 1:1:1 produced the largest amount of indole-3-acetic acid (15.59 mg/mL) and accumulated maximum biomass. The addition of 0.2% L-tryptophan to the nutrient medium increased the amount of indole-3-acetic acid to 18.45 mg/mL. When the T. prantense L. seeds were soaked in the biopreparation (consortium’s culture fluid) at a concentration of 2.5, the sprouts were 1.4 times longer on the 10th day of growth, compared to the control. The consortium of Pantoea spp., E. faecium, and R. endophytica (1:1:1) stimulated the growth of T. prantense L. seeds. Our findings can be further used to develop biofertilizers for agriculture.
The use of fruit raw materials of the Siberian region as the basis of fillings for bakery products
The results of the study of fruits and their products as sources of antioxidant substances (vitamins, organic acids and various phenolic substances) in the human diet are presented. It was found that there is a variation in the biologically valuable substances contained in fruits depending on the place of growth, cultivation and other conditions. The purpose of the study is to assess the feasibility of using traditional for the Siberian region fruit raw materials (cranberry, lingonberry and chokeberry) as the basis for the filling of bakery products. The object of research was fresh wild fruits of cranberry and lingonberry (harvest of 2021, harvested by the Tomsk Regional Consumer Union of the Russian Federation) and chokeberry fruits (variety mix, grown in the Kemerovo region and sold in the consumer market). The organoleptic evaluation of the fillings was carried out on a 5-point scale, total antioxidant activity - by coulometric express method on the device "Express-006- Antioxidants". Based on the studies conducted, it was found that fresh fruits are ranked as follows in terms of antioxidant activity: cranberry > chokeberry > lingonberry. It was found that the indicator "appearance and consistency" of fillings for bakery products is mainly influenced by structural formers, which are ranked as follows: 1.0% agar > 2.0 % pectin > 1.5 % agar > 6.0 % modified starch. The smell and taste of fillings are mainly influenced by the fruit raw material used, and the structure-forming agents are indirectly influenced by them. Total antioxidant activity of bakery fillings depends on the destruction of biologically active substances of fruit during manufacturing, rather than on the used structure-forming agents. The results of these studies will be useful in the creation of functional food products.
The Quality and Safety of Goat Milk Sour-Milk Product
To improve the quality of life of the population, currently a promising direction is the use of functional foods. The article presents the results of the conducted analysis of the quality and safety of symbiotic yogurt from goat milk enriched with a mixture of vegetable raw materials.Purpose of the study: to study the effect of quince syrup on the quality and safety of fermented milk products.This paper presents the results of the study of the developed fermented milk product (yogurt) for functional purposes. The composition of the added ready-made starter includes the cultures Str.thermophiIlus, Lb.bulgaricus, Lb. acidophilus. Quince fruit syrup was used as a natural ingredient of plant origin.Addition of quince syrup in the production of yogurt from goat's milk contributes to obtaining a product enriched with dietary fiber, vitamins, macro-microelements, as well as increasing the range and improving the organoleptic properties of dairy products, including pectin-containing.The presented article provides comprehensive microbiological indicators of the studied yogurt samples and the dynamics of changes in lactic acid microflora during storage.The results showed that according to microbiological indicators, the yoghurts met the requirements of TR CU 033/2013, QMAFAnM did not exceed the specified standards, and coliform bacteria were absent. The growth of lactic acid bacteria in the studied samples was intense on the fifth day – 6.4×109 CFU, then the number of microorganisms decreased. On the seventh day, the number of lactic acid bacteria in the studied sample was – 4.5×108 CFU, the indicator of the control sample for this period was – 2.4×107 CFU.
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