Plant‐mediated RNAi of grain aphid <i>CHS1</i> gene confers common wheat resistance against aphids

Zhao, Yanjie; Sui, Xiao-Yan; Xu, Li; Liu, Guoyu; Lü, Lihua; You, Minsheng; Xie, Chaojie; Li, Baoyun; Ni, Zhongfu; Liang, Rongqi

doi:10.1002/ps.5062

Cited by 37 publications

(34 citation statements)

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“…However, in soybean aphid (A. glycines Matsumura), the mRNA transcription of CHS1 gene was highest in the nymphal stage (second instar) [41]. Similarly, the peak expression level of CHS1 was also detected during developmental stages of grain aphid (Sitobion avenae) [39]. In the present study, transcripts of CHS1 gene were perceived in all life stages of A. gossypii; however, the highest expression was observed in the first-instar nymph.…”

Section: Discussionsupporting

confidence: 41%

“…CHS1 is expressed in the epidermal cells encoding enzymes that are essential for the catalysis of chitin production in cuticle [36,37], while CHS2 is expressed in the peritrophic membrane regulating enzymes for chitin production in insect midgut [38]. Several studies reported that CHS1 gene is crucial for chitin synthesis in aphid molting [39][40][41]. However, due to lacking peritrophic membrane, CHS2 was not existent in some insects [40][41][42].…”

Section: Introductionmentioning

confidence: 99%

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RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

Ullah

Gül

Wang

et al. 2019

Insects

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Chitin is a vital part of the insect exoskeleton and peritrophic membrane, synthesized by chitin synthase (CHS) enzymes. Chitin synthase 1 (CHS1) is a crucial enzyme in the final step of chitin biosynthetic pathway and consequently plays essential role towards insect growth and molting. RNA interference (RNAi) is an agent that could be used as an extremely target-specific and ecologically innocuous tactic to control different insect pests associated with economically important crops. The sole purpose of the current study is to use CHS1 as the key target gene against the cotton-melon aphid, Aphis gossypii, via oral feeding on artificial diets mixed with dsRNA-CHS1. Results revealed that the expression level of CHS1 gene significantly decreased after the oral delivery of dsRNA-CHS1. The knockdown of CHS1 gene caused up to 43%, 47%, and 59% mortality in third-instar nymph after feeding of dsCHS1 for 24, 48, and 72 h, respectively, as compared to the control. Consistent with this, significantly lower longevity (approximately 38%) and fecundity (approximately 48%) were also found in adult stage of cotton-melon aphids that were fed with dsCHS1 for 72 h at nymphal stage. The qRT-PCR analysis of gene expression demonstrated that the increased mortality rates and lowered longevity and fecundity of A. gossypii were attributed to the downregulation of CHS1 gene via oral-delivery-mediated RNAi. The results of current study confirm that CHS1 could be an appropriate candidate target gene for the RNAi-based control of cotton-melon aphids.

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Section: Discussionsupporting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

Ullah

Gül

Wang

et al. 2019

Insects

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“…Therefore, given the level of sequence divergence between the corresponding M. persicae genes and the Be. tabaci ecdysone receptor and P. solenopsis chitin synthase constructs (Table S1), the RNAi effects on the expression levels of these genes are likely to be more minor than those observed with M. persicae cathepsin L. Moreover, we did not observe the morphological phenotypes that were previously reported when silencing ecdysone receptor or chitin synthase expression (Zhu et al , ; Khan et al , ; Shang et al , ; Yan et al , ; Vellichirammal et al , ; Zhao et al , ), and the identified protein accumulation deficit is more similar to what one would expect from silencing the expression of a gut protease.…”

Section: Discussionmentioning

confidence: 50%

“…Moreover, a C ‐> U mismatch at such a position is predicted to have a severe effect on RNAi efficiency (Ahmed and Raghava, ). Previous experiments involving expression silencing of chitin synthase in aphids and mealybugs resulted in physical deformities and/or the inability to moult (Khan et al , ; Shang et al , ; Zhao et al , ). We did not observe such morphological effects in our M. persicae experiments.…”

Section: Resultsmentioning

confidence: 99%

Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata

Rauf

Asif

Amin

et al. 2019

Insect Molecular Biology

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Gut‐expressed aphid genes, which may be more easily inhibited by RNA interference (RNAi) constructs, are attractive targets for pest control efforts involving transgenic plants. Here we show that expression of cathepsin L, which encodes a cysteine protease that functions in aphid guts, can be reduced by expression of an RNAi construct in transgenic tobacco. The effectiveness of this approach is demonstrated by up to 80% adult mortality, reduced fecundity, and delayed nymph production of Myzus persicae (green peach aphids) when cathepsin L expression was reduced by plant‐mediated RNAi. Consistent with the function of cathepsin L as a gut protease, M. persicae fed on the RNAi plants had a lower protein content in their bodies and excreted more protein and/or free amino acids in their honeydew. Larvae of Coccinella septempunctata (seven‐spotted ladybugs) grew more slowly on aphids having reduced cathepsin L expression, suggesting that prey insect nutritive value, and not just direct negative effects of the RNAi construct, needs to be considered when producing transgenic plants for RNAi‐mediated pest control.

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“…The production of transgenic plants is another strategy to deliver dsRNA for inset pest control. A number of studies have used this approach to develop transgenic plants expressing dsRNA against insects in the order Hemiptera [40][41][42], Coleoptera [17,[43][44][45], or Lepidoptera [16,46,47]. However, transgenic plant-mediated RNAi based on conventional nuclear transformation technology did not offer efficient protection from insect damage, probably due to (i) low expression of dsRNA and (ii) instability of dsRNAs (result from plants' endogenous Dicer cleavage) in planta [14].…”

Section: Discussionmentioning

confidence: 99%

Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)

Zhang

et al. 2019

Insects

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RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five target genes tested and the suppression of ACT and SRP54 genes caused significant mortality. Our results suggest that the oral delivery of bacteria-expressed dsRNA is a potential alternative for the control of P. versicolora, and that ACT and SRP54 genes are the potent targets.

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Plant‐mediated RNAi of grain aphid CHS1 gene confers common wheat resistance against aphids

Abstract: These results indicate that plant-mediated RNAi of the grain aphid CHS1 gene confers common wheat resistance against aphids. © 2018 Society of Chemical Industry.

Cited by 37 publications

References 37 publications

RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

RNAi-Mediated Knockdown of Chitin Synthase 1 (CHS1) Gene Causes Mortality and Decreased Longevity and Fecundity in Aphis gossypii

Silencing cathepsin L expression reduces Myzus persicae protein content and the nutritional value as prey for Coccinella septempunctata

Bacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae)

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