Plant Metabolomics: An Overview of Technology Platforms for Applications in Metabolism

Sangwan, Neelam S.; Tiwari, Pragya; Mishra, Siddhartha; Yadav, Radha; Tripathi, Swati; Kushwaha, A. K. S.; Sangwan, Rajender Singh

doi:10.1007/978-81-322-2172-2_9

Cited by 6 publications

(6 citation statements)

References 129 publications

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“…The observed richness of rose‐scented geranium essential oil in monoterpenes (such as citronellol and geraniol) connotate this relative participation/contribution of the two pathways in rose‐scented geranium relates to monoterpene production. Essential oil biosynthesis is highly integrated with the physiological and differentiating stage of the plant and it is also affected by the ecological conditions (Sangwan et al , , ). Geraniol was present in highest amount in unexpanded leaves and afterwards percentage was continuously decreased up to fully expanded stage of leaf.…”

Section: Discussionmentioning

confidence: 99%

Over‐expression of DXS gene enhances terpenoidal secondary metabolite accumulation in rose‐scented geranium and Withania somnifera: active involvement of plastid isoprenogenic pathway in their biosynthesis

Jadaun

Sangwan

Narnoliya

et al. 2016

Physiologia Plantarum

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Rose-scented geranium (Pelargonium spp.) is one of the most important aromatic plants and is well known for its diverse perfumery uses. Its economic importance is due to presence of fragrance rich essential oil in its foliage. The essential oil is a mixture of various volatile phytochemicals which are mainly terpenes (isoprenoids) in nature. In this study, on the geranium foliage genes related to isoprenoid biosynthesis (DXS, DXR and HMGR) were isolated, cloned and confirmed by sequencing. Further, the first gene of 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, 1-deoxy-d-xylulose-5-phosphate synthase (GrDXS), was made full length by using rapid amplification of cDNA ends strategy. GrDXS contained a 2157 bp open reading frame that encoded a polypeptide of 792 amino acids having calculated molecular weight 77.5 kDa. This study is first report on heterologous expression and kinetic characterization of any gene from this economically important plant. Expression analysis of these genes was performed in different tissues as well as at different developmental stages of leaves. In response to external elicitors, such as methyl jasmonate, salicylic acid, light and wounding, all the three genes showed differential expression profiles. Further GrDXS was over expressed in the homologous (rose-scented geranium) as well as in heterologous (Withania somnifera) plant systems through genetic transformation approach. The over-expression of GrDXS led to enhanced secondary metabolites production (i.e. essential oil in rose-scented geranium and withanolides in W. somnifera). To the best of our knowledge, this is the first report showing the expression profile of the three genes related to isoprenoid biosynthesis pathways operated in rose-scented geranium as well as functional characterization study of any gene from rose-scented geranium through a genetic transformation system.

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Section: Discussionmentioning

confidence: 99%

Over‐expression of DXS gene enhances terpenoidal secondary metabolite accumulation in rose‐scented geranium and Withania somnifera: active involvement of plastid isoprenogenic pathway in their biosynthesis

Jadaun

Sangwan

Narnoliya

et al. 2016

Physiologia Plantarum

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“…Following this, methodologies have been established for carotenoid metabolomics using time of flight (Fraser et al 2007) and orbitrap MS (Bijttebier et al 2013(Bijttebier et al , 2014Van Meulebroek et al 2014). For more details on the various steps of the metabolomics workflow (including data acquisition, data pre-processing, multivariate statistical analysis, metabolite identification, and biological interpretation), we refer to the reviews of Hegeman (2010), Hendriks et al (2011), Neumann andB€ ocker (2010), and Sangwan et al (2015). Up to now, only a few studies have reported on the use of metabolomics to address carotenoidrelated research questions (Chu et al 2011;Djuric et al 2009;Lamers et al 2010;Lee and Park 2010;Sawada et al 2019).…”

Section: Metabolomics Analysis In Carotenoid Researchmentioning

confidence: 99%

A comprehensive review on carotenoids in foods and feeds: status quo, applications, patents, and research needs

Meléndez-Martínez

Mandić

Bantis

et al. 2021

Critical Reviews in Food Science and Nutrition

191

107

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Carotenoids are isoprenoids widely distributed in foods that have been always part of the diet of humans. Unlike the other so-called food bioactives, some carotenoids can be converted into retinoids exhibiting vitamin A activity, which is essential for humans. Furthermore, they are much more versatile as they are relevant in foods not only as sources of vitamin A, but also as natural pigments, antioxidants, and health-promoting compounds. Lately, they are also attracting interest in the context of nutricosmetics, as they have been shown to provide cosmetic benefits when ingested in appropriate amounts. In this work, resulting from the collaborative work of participants of the COST Action European network to advance carotenoid research and applications in agrofood and health (EUROCAROTEN, www.eurocaroten.eu, https://www.cost.eu/actions/CA15136/ #tabsjName:overview) research on carotenoids in foods and feeds is thoroughly reviewed covering aspects such as analysis, carotenoid food sources, carotenoid databases, effect of processing and storage conditions, new trends in carotenoid extraction, daily intakes, use as human, and feed additives are addressed. Furthermore, classical and recent patents regarding the obtaining and formulation of carotenoids for several purposes are pinpointed and briefly discussed. Lastly, emerging research lines as well as research needs are highlighted.

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“…Phytosterols, such as stigmasterol, campesterol, and sitosterol, are a large class of organic compounds, known as sterols or steroidal alcohols, found in plants. In addition, phytosterols have been shown in clinical trials to block cholesterol absorption sites in the human intestine, lowering cholesterol levels [ 47 ]. Elephant apple leaves are found to contain flavonoids, tannins, triterpenoids, and steroids; petroleum extract of elephant apple leaves is found to contain sitosterol, cycloartenone, betulin, and n-hentriacontanol, while chloroform extract is found to contain betulinic acid [ 48 ].…”

Section: Classification and Chemistrymentioning

confidence: 99%

Bioactive Compounds and Health-Promoting Properties of Elephant Apple (Dillenia indica L.): A Comprehensive Review

Saikia

Radhakrishnan

Inbaraj

et al. 2023

Foods

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Elephant apple (Dillenia indica L.) grows wild in Southeast Asia’s forests, including in China, India, Nepal, Bangladesh, and Sri Lanka. Elephant apples are considered essential fruit crops because of their high nutritional value, which includes high levels of vitamin C, carbohydrates, fats, fibre, protein, minerals, and fatty acids. It is important to understand the nutritional value and health benefits of elephant apples in order to increase fruit intake in people’s daily diets. The present review paper focuses on elephant apple’s phytochemistry, bioactive compounds, therapeutic value, and medicinal capabilities for designing and developing a wide range of food formulations. Proteins, minerals, fats, crude fibre, carbohydrates, vitamin C, tannins, malic acid, and glucose are abundant in the leaves, bark, and fruit of the elephant apple. In addition to nutritional components, many phytochemicals found in elephant apples have been identified as bioactive compounds with a broad range of biological activities, the most prominent of which are antioxidant, anticancer, antidiabetic, and anti-inflammatory properties. Overall, elephant apple is a rich, natural source of bioactive compounds with potential applications in the production of value-added foods and nutraceuticals for disease prevention and management.

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Plant Metabolomics: An Overview of Technology Platforms for Applications in Metabolism

Cited by 6 publications

References 129 publications

Over‐expression of DXS gene enhances terpenoidal secondary metabolite accumulation in rose‐scented geranium and Withania somnifera: active involvement of plastid isoprenogenic pathway in their biosynthesis

Over‐expression of DXS gene enhances terpenoidal secondary metabolite accumulation in rose‐scented geranium and Withania somnifera: active involvement of plastid isoprenogenic pathway in their biosynthesis

A comprehensive review on carotenoids in foods and feeds: status quo, applications, patents, and research needs

Bioactive Compounds and Health-Promoting Properties of Elephant Apple (Dillenia indica L.): A Comprehensive Review

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