Plant microProteins: Small but powerful modulators of plant development

Kushwaha, A. K. S.; Dwivedi, Shubhi; Mukherjee, Arpan; Lingwan, Maneesh; Dar, Mansoor Ali; Bhagavatula, Lavanya; Datta, Sourav

doi:10.1016/j.isci.2022.105400

Cited by 14 publications

(10 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This domain allows plant microProteins to interact with other compatible domains of evolutionary-related proteins and perform specific key physiological effects in several organisms. In particular, microProteins are recently under deep investigation, in consideration of their role in plant development [ 69 , 70 ]. Viscotoxins are cationic cysteine-rich proteins composed of only 45 amino acids and with a molecular weight less than 5 KDa [ 67 ].…”

Section: Introductionmentioning

confidence: 99%

“…The presence and content of Viscum microProteins, and therefore their content in related marketed products, is subject to several variations. Although the foliage (meaning leaves, little branches and shoots when present) content is higher than the fruit one, again the percentage is subject to high variation in relation to the subspecies, the host, the season and the ecological conditions [ 70 ], assigning importance to the harvesting time. It is expected that the pharmacological activities of mistletoe extracts, such as cytotoxicity and induction of apoptosis in cancer cells, vary accordingly.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Anti-Inflammatory Activity of Viscum album

Nicoletti

2023

Plants

View full text Add to dashboard Cite

The therapeutic story of European mistletoe (Viscum album L.) presents a seesawing profile. In ancient times, this hemiparasitic plant was considered a panacea and even to be endowed with exceptional beneficial properties. In more recent times, despite its multiple uses in traditional medicines, some parts of the plant, in particular the berries, were considered poisonous and dangerous, including concerns of cytotoxicity, which spread serious suspicion on its medicinal utility. However, since the last century, medical interest in mistletoe has come back in force due to its utilization in clinical cancer treatments, based on its selective action on tumor cells. In Central Europe, the hydro-alcoholic extracts of European mistletoe register a relevant and continuous utilization in anthroposophic medicine, which is a holistic system that includes the utilization of phytomedicinal substances. In Switzerland and Germany, most physicians and patients use these products as complementary therapy in oncological treatments. However, despite its increasing use in this field, the results of mistletoe’s use are not always convincing, and other aspects have appeared. Nowadays, products that contain mistletoe are utilized in several fields, including diet, phytotherapy, veterinary medicine and homeopathy, but in particular in cancer therapies as coadjuvant factors, in consideration of several positive effects including effects in the improvement of quality-of-life conditions and reinforcement of the immune system. In this review, based on the understanding of the association between cancer and inflammation, we propose a relationship between these recent uses of mistletoe, based on its antioxidant properties, which are supported by phytochemical and pharmacological data. The unicity of mistletoe metabolism, which is a direct consequence of its hemiparasitism, is utilized as a key interpretation element to explain its biological properties and steer its consequent therapeutic uses.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Anti-Inflammatory Activity of Viscum album

Nicoletti

2023

Plants

View full text Add to dashboard Cite

show abstract

“…Regulation by microProteins has emerged as a relatively new mechanism of post‐translational control of protein abundance and activity in various organisms (Rodrigues et al ., 2021; Kruusvee et al ., 2022; Kruusvee & Wenkel, 2022; Kushwaha et al ., 2022). MicroProteins are small, single‐domain proteins generally < 140 amino acids long that share sequence homology with multidomain proteins (Hong et al ., 2005; Dolde et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

BBX30/miP1b and BBX31/miP1a form a positive feedback loop with ABI5 to regulate ABA‐mediated postgermination seedling growth arrest

Singh

Datta

2023

New Phytologist

Self Cite

View full text Add to dashboard Cite

Summary In plants, the switch to autotrophic growth involves germination followed by postgermination seedling establishment. When environmental conditions are not favorable, the stress hormone abscisic acid (ABA) signals plants to postpone seedling establishment by inducing the expression of the transcription factor ABI5. The levels of ABI5 determine the efficiency of the ABA‐mediated postgermination developmental growth arrest. The molecular mechanisms regulating the stability and activity of ABI5 during the transition to light are less known. Using genetic, molecular, and biochemical approach, we found that two B‐box domain containing proteins BBX31 and BBX30 alongwith ABI5 inhibit postgermination seedling establishment in a partially interdependent manner. BBX31 and BBX30 are also characterized as microProteins miP1a and miP1b, respectively, based on their small size, single domain, and ability to interact with multidomain proteins. miP1a/BBX31 and miP1b/BBX30 physically interact with ABI5 to stabilize it and promote its binding to promoters of downstream genes. ABI5 reciprocally induces the expression of BBX30 and BBX31 by directly binding to their promoter. ABI5 and the two microProteins thereby form a positive feedback loop to promote ABA‐mediated developmental arrest of seedlings.

show abstract

“…COP1 ubiquitinates ABD1 to promote its degradation and thereby enhances ABI5 protein stability in dark(Peng et al, 2022). Additionally, COP1 promotes the binding of ABI5 to its target promoters to inhibit seedling growth (Yadukrishnan et al, 2020).·Regulation by microProteins has emerged as a relatively new mechanism of post-translational control of protein abundance and activity in various organisms (Rodrigues et al, 2021; Kruusvee et al, 2022; Kruusvee and Wenkel, 2022; Kushwaha et al, 2022). MicroProteins are small, single-domain proteins generally less than 140 amino acids long that share sequence homology with multi-domain proteins (Hong et al, 2005; Dolde et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Regulation by microProteins has emerged as a relatively new mechanism of post-translational control of protein abundance and activity in various organisms (Rodrigues et al, 2021;Kushwaha et al, 2022). MicroProteins are small, single-domain proteins generally less than 140 amino acids long that share sequence homology with multi-domain proteins (Hong et al, 2005;Dolde et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

MicroProteins miP1b/BBX30 and miP1a/BBX31 form a positive feedback loop with ABI5 to retard seedling establishment

Singh

Datta

2022

Preprint

Self Cite

View full text Add to dashboard Cite

In plants the switch to autotrophic growth involves germination followed by post-germination seedling establishment. When the environmental conditions are not favourable the stress hormone Abscisic Acid (ABA) signals plants to postpone seedling establishment by inducing the expression of the transcription factor ABI5. The levels of ABI5 determine the efficiency of the ABA mediated post-germination developmental growth arrest. The molecular mechanisms regulating the stability and activity of ABI5 during the transition to light is less known. We found that two microProteins miP1a/ BBX31 and miP1b/BBX30 along with ABI5 inhibit post-germination seedling establishment in a partially interdependent manner. MicroProteins are single-domain proteins that interact with multi-domain proteins to modulate their post-translational activity. miP1a/ BBX31 and miP1b/BBX30 physically interact with ABI5 to stabilize it and promote its binding to promoters of downstream genes in light. ABI5 reciprocally induces the expression of BBX30 and BBX31 by directly binding to their promoter. ABI5 and the two microProteins thereby form a positive feedback loop to promote ABA-mediated developmental arrest of seedlings. Our study highlights the functional versatility of microProteins which can act as regulators of crucial developmental transitions in plants as well as other eukaryotes.

show abstract

Plant microProteins: Small but powerful modulators of plant development

Cited by 14 publications

References 94 publications

The Anti-Inflammatory Activity of Viscum album

The Anti-Inflammatory Activity of Viscum album

BBX30/miP1b and BBX31/miP1a form a positive feedback loop with ABI5 to regulate ABA‐mediated postgermination seedling growth arrest

MicroProteins miP1b/BBX30 and miP1a/BBX31 form a positive feedback loop with ABI5 to retard seedling establishment

Contact Info

Product

Resources

About