Plant MicroRNAs in Cross-Kingdom Regulation of Gene Expression

Wang, Wei; Liu, Dan; Zhang, Xiaopei; Chen, Dongdong; Cheng, Yingying; Shen, Fafu

doi:10.3390/ijms19072007

Cited by 45 publications

(38 citation statements)

References 74 publications

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“…However, beside these predictions, no study so far has firmly demonstrated the functionality of plant-derived miRNA on the mammalian genome and it is still a matter of debate. 45 In the intestinal lumen, dietary TGs are modified into monoacylglycerol and fatty acids, which are incorporated into micelles and taken up by enterocytes. Once in the enterocytes, monoacylglycerol and fatty acids are re-esterified into TGs and packaged into chylomicrons for secretion.…”

Section: Discussionmentioning

confidence: 99%

Use of Nanovesicles from Orange Juice to Reverse Diet-Induced Gut Modifications in Diet-Induced Obese Mice

Berger

Colosetti

Jalabert

et al. 2020

Molecular Therapy - Methods & Clinical Development

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We have determined whether orange juice-derived nanovesicles (ONVs) could be used for the treatment of obesity-associated intestinal complications. ONVs were characterized by lipidomic, metabolomic, electron microscopy. In vitro, intestinal barriers (IBs = Caco-2+HT-29-MTX) were treated with ONVs and co-cultured with adipocytes to monitor IB fat release. In vivo, obesity was induced with a high-fat, high-sucrose diet (HFHSD mice) for 12 weeks. Then, half of HFHSD mice were gavaged with ONVs. One-month ONV treatment did not modify HFHSD-induced insulin resistance but reversed diet-induced gut modifications. In the jejunum, ONVs increased villi size, reduced triglyceride content, and modulated mRNA levels of genes involved in immune response (tumor necrosis factor [TNF]-a and interleukin [IL]-1b), barrier permeability (CLDN1, OCLN, ZO1), fat absorption, and chylomicron release. ONVs targeted microsomal triglyceride transfer protein (MTP) and angiopoietin-like protein-4 (ANGPTL4), two therapeutic targets to reduce plasma lipids and inflammation in gastrointestinal diseases. Interestingly, ONV treatment did not aggravate liver steatosis, as MTP mRNA was increased in the liver. Therefore, ONVs protected both intestine and the liver from fat overload associated with the HFHSD. As ONVs concentrated amino acids and bioactive lipids versus orange juice, which are deficient in obese patients, the use of ONVs as a dietary supplement could bring physiological relevant compounds in the jejunum to accelerate the restoration of intestinal functions during weight loss in obese patients.

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Section: Discussionmentioning

confidence: 99%

Use of Nanovesicles from Orange Juice to Reverse Diet-Induced Gut Modifications in Diet-Induced Obese Mice

Berger

Colosetti

Jalabert

et al. 2020

Molecular Therapy - Methods & Clinical Development

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“…The inter-species regulation of miRNAs has already been shown in many virus and parasites (6). Early study in 2004 shown that virus could encode miRNAs and regulate host gene expression (19).…”

Section: Discussionmentioning

confidence: 97%

“…Such phenomena are highlighted as the cross-kingdom regulation of plant miRNAs (3). It is believed that these plant-derived miRNAs have roles in human biological processes and disease (6). In 2007, we built the human miRNA disease database (HMDD) (7) and proposed version 2 and 3 in 2014 and this year (8,9).…”

Section: Introductionmentioning

confidence: 99%

RPRH: a web-based tool for the inference of potential roles of plant microRNAs in human body

Gao

Jia

Cui

et al. 2020

Preprint

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Recently, increasing studies reported that plant microRNAs (miRNAs) from food could be absorbed through gastrointestinal tract to regulate the physiological processes of animals. These novel cross-kingdom regulatory roles of plant miRNAs open a new field in miRNA study and drug discovery, but the lack of corresponding bioinformatics tools limit the development of this field. In this study, we built a web-based tool, RPRH (Role of Plant microRNA in Human), to infer the potential roles of plant miRNAs in human and to profile the landscape of all documented plant miRNAs regulatory functions in human. Totally, RPRH included 10414 miRNAs from 82 plant species and 18062 annotation terms like diseases, pathway, gene ontology (GO), and drugs. RPRH represents a bioinformatics resource for further investigation of cross-kingdom regulations of plant miRNAs. Availability: http://www.rnanut.net/rprh/

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“…The small RNAs are a diverse class of non-coding regulatory RNAs with important functions by regulating gene expression through targeting the RNA transcripts of endogenous as well as exogenous genes involved in stress response [94,95]. Growing evidence reveals that miRNA-guided regulation of ROS-related genes at the post-transcriptional level is essential for normal growth and development [96], and adaptation to stress conditions including salinity [97,98].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Mining and Identification of Protein Kinase Gene Family Impacts Salinity Stress Tolerance in Highly Dense Genetic Map Developed from Interspecific Cross between G. hirsutum L. and G. darwinii G. Watt

et al. 2019

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Abiotic stress is an important limiting factor in crop growth and yield around the world. Owing to the continued genetic erosion of the upland cotton germplasm due to intense selection and inbreeding, attention has shifted towards wild cotton progenitors which offer unique traits that can be introgressed into the cultivated cotton to improve their genetic performance. The purpose of this study was to characterize the Pkinase gene family in a previously developed genetic map of the F2 population derived from a cross between two cotton species: Gossypium hirsutum (CCRI 12-4) and Gossypium darwinii (5-7). Based on phylogenetic analysis, Pkinase (PF00069) was found to be the dominant domain with 151 genes in three cotton species, categorized into 13 subfamilies. Structure analysis of G. hirsutum genes showed that a greater percentage of genes and their exons were highly conserved within the group. Syntenic analysis of gene blocks revealed 99 duplicated genes among G. hirsutum, Gossypium arboreum and Gossypium raimondii. Most of the genes were duplicated in segmental pattern. Expression pattern analysis showed that the Pkinase gene family possessed species-level variation in induction to salinity and G. darwinii had higher expression levels as compared to G. hirsutum. Based on RNA sequence analysis and preliminary RT-qPCR verification, we hypothesized that the Pkinase gene family, regulated by transcription factors (TFs) and miRNAs, might play key roles in salt stress tolerance. These findings inferred comprehensive information on possible structure and function of Pkinase gene family in cotton under salt stress.

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Plant MicroRNAs in Cross-Kingdom Regulation of Gene Expression

Cited by 45 publications

References 74 publications

Use of Nanovesicles from Orange Juice to Reverse Diet-Induced Gut Modifications in Diet-Induced Obese Mice

Use of Nanovesicles from Orange Juice to Reverse Diet-Induced Gut Modifications in Diet-Induced Obese Mice

RPRH: a web-based tool for the inference of potential roles of plant microRNAs in human body

Genome-Wide Mining and Identification of Protein Kinase Gene Family Impacts Salinity Stress Tolerance in Highly Dense Genetic Map Developed from Interspecific Cross between G. hirsutum L. and G. darwinii G. Watt

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