Plant microtubules reorganization under the indirect UV-B exposure and during UV-B-induced programmed cell death

Krasylenko, Yuliya; Yemets, А. І.; Blume, Ya. B.

doi:10.4161/psb.24031

Cited by 32 publications

(20 citation statements)

References 29 publications

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“…Such rapid signalling through Trp conduction pathways may coordinate the complex organization of the microtubule cytoskeleton required for the tasks of cell division, motor protein trafficking and motility. Microtubules have been shown to reorganize in a dose-dependent manner after exposure to UV light [39][40][41][42], with the greatest effect being observed around 280 nm [42]. Feasible mechanisms for these changes include the reduction of disulfide or peptide bonds induced by photoexcitation of Trp groups [43][44][45][46], or subtle protein structural changes owing to photo-induced alterations in Trp flexibility [46].…”

Section: Resultsmentioning

confidence: 99%

The feasibility of coherent energy transfer in microtubules

Craddock

Friesen

Mane

et al. 2014

J. R. Soc. Interface.

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It was once purported that biological systems were far too 'warm and wet' to support quantum phenomena mainly owing to thermal effects disrupting quantum coherence. However, recent experimental results and theoretical analyses have shown that thermal energy may assist, rather than disrupt, quantum coherent transport, especially in the 'dry' hydrophobic interiors of biomolecules. Specifically, evidence has been accumulating for the necessary involvement of quantum coherent energy transfer between uniquely arranged chromophores in light harvesting photosynthetic complexes. The 'tubulin' subunit proteins, which comprise microtubules, also possess a distinct architecture of chromophores, namely aromatic amino acids, including tryptophan. The geometry and dipolar properties of these aromatics are similar to those found in photosynthetic units indicating that tubulin may support coherent energy transfer. Tubulin aggregated into microtubule geometric lattices may support such energy transfer, which could be important for biological signalling and communication essential to living processes. Here, we perform a computational investigation of energy transfer between chromophoric amino acids in tubulin via dipole excitations coupled to the surrounding thermal environment. We present the spatial structure and energetic properties of the tryptophan residues in the microtubule constituent protein tubulin. Plausibility arguments for the conditions favouring a quantum mechanism of signal propagation along a microtubule are provided. Overall, we find that coherent energy transfer in tubulin and microtubules is biologically feasible.

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Section: Resultsmentioning

confidence: 99%

The feasibility of coherent energy transfer in microtubules

Craddock

Friesen

Mane

et al. 2014

J. R. Soc. Interface.

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“…Disruption of plant microtubules induced by enhanced UV-B radiation is accompanied by cell shrinkage, chromatin condensation, and cytoplasmic vacuolation in both interphase and the mitotic phase (Lytvyn et al, 2010). Plant microtubules also participate in UV-B signaling pathways as a responsive factor (Krasylenko et al, 2013 ) causes depolymerization of cortical microtubules and aggregation of arrays of spindle microtubules (Guo et al, 2010). In addition, the frequency of chromosomal aberrations is increased, including chromosome bridges and laggard chromosomes and leads to partition-bundle division (a novel type of chromosomal aberration, in which chromosomes at the transition from anaphase to telophase of mitosis are divided into three, four or six bundles) (Rong et al, 2002;Gao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of Enhanced Ultraviolet-B Radiation on Chromosomes and Microtubule Arrays in Wheat Roots

Chen¹,

Chen²,

Han³

2016

IJAB

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To cite this paper: Chen, P.X., H. Chen and R. Han, 2016 AbstractEnhanced ultraviolet-B (UV-B) radiation causes chromosomal aberrations and abnormal microtubule arrays in wheat (Triticum aestivum) root cells. Oryzalin induces chromosome doubling through depolymerization of microtubules and results in diploidization. We explored the effect of oryzalin on wheat roots exposed to enhanced UV-B radiation. Treatment with 500 µmol·L −1 oryzalin resulted in chromosome doubling with two equal nuclei. However, exposure to UV-B radiation in combination with oryzalin resulted in two unequal nuclei. The frequency of unequal nuclei caused by treatment with UV-B and oryzalin was higher than that caused by UV-B exposure alone. During the mitotic phase, chromosomal aberrations were indicated to be independent of abnormal microtubule structures. These suggested that chromosomal aberrations caused by enhanced UV-B radiation were not a consequence of abnormal microtubule structures.

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“…Studies have shown that UV-B induces MT depolymerization and leads to a series of effects (Guo et al 2010;Krasylenko et al 2012;Krasylenko et al 2013). However, the mechanisms of depolymerization of MTs under UV-B remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Microtubule Organization in Arabidopsis thaliana TUBGFP and MBD-GFP Mutants Exposed to UV-B Radiation

Chen

Han

2017

Tarım Bilimleri Dergisi

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Microtubule organization was compared between the Arabidopsis thaliana TUB-GFP and MBD-GFP mutants. Plant height and primary root length were measured, and microtubule dynamics were examined by confocal laser scanning microscopy after UV-B radiation to reveal changes in microtubules. Damage caused by UV-B was comparable between transgenic lines and wild-type plants, although transgenic lines were more sensitive to UV-B than the wild-type. Spots and depolymerization of microtubules were detected in both TUB-GFP and MBD-GFP plants; however, MBD-GFP showed better adaptation of changes induced by UV-B treatment. These results indicated that UV-B inhibits the growth and development of transgenic lines, and the inhibitory effects might result from changes in microtubules, as determined by comparison between the TUB-GFP and MBD-GFP lines.

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Plant microtubules reorganization under the indirect UV-B exposure and during UV-B-induced programmed cell death

Cited by 32 publications

References 29 publications

The feasibility of coherent energy transfer in microtubules

The feasibility of coherent energy transfer in microtubules

Effects of Enhanced Ultraviolet-B Radiation on Chromosomes and Microtubule Arrays in Wheat Roots

Comparison of Microtubule Organization in Arabidopsis thaliana TUBGFP and MBD-GFP Mutants Exposed to UV-B Radiation

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