Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector

Tzelepis, Georgios; Dölfors, Fredrik; Holmquist, Louise; Dixelius, Christina

doi:10.1016/j.bbrc.2021.01.019

Cited by 17 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Abnormality of COX would be critical for plant cells, leading to cell death as a defence reaction. In addition to RsIA_CtaG/Cox11, the mitochondria‐targeted effector RsCRP1 has been identified as a virulence factor in R. solani (Tzelepis et al., 2021). However, no induction of necrosis or suppression of cell death by RsCRP1 was observed.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, RsIA_CtaG/Cox11 (AG1IA_05310), RsIA_Peptidase_S8 (AG1IA_07795), and RsIA_GT (AG1IA_09161) were experimentally verified to have necrosis‐inducing activity by infiltrating recombinant proteins produced in Escherichia coli into rice leaves (Zhang et al., 2022; Zheng et al., 2013). Subsequent studies have experimentally verified the function of effector candidates in R. solani (Dong & Wang, 2016; Ghosh et al., 2018; Rao et al., 2020), including AGLIP1, RSAG8, RsIA_NP8, RSSCR10, RsLysM, RsRIpA, and RsCRP1 (Anderson et al., 2017; Charova et al., 2020; Dölfors et al., 2019; Li et al., 2019; Niu et al., 2021; Tzelepis et al., 2021; Wei et al., 2020). Potential effectors have also been explored using different approaches, mainly from AG‐1 IA (Abdelsalam et al., 2020; Dong & Wang, 2016; Ghosh et al., 2018; Niu et al., 2023; Rao et al., 2020; Xia et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mitochondrial‐targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG‐1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit

Zhang,

Lin,

Yamamoto

et al. 2023

Molecular Plant Pathology

View full text Add to dashboard Cite

Rhizoctonia solani AG‐1 IA causes a necrotrophic rice disease and is a serious threat to rice production. To date, only a few effectors have been characterized in AG‐1 IA. We previously identified RsIA_CtaG/Cox11 and showed that infiltration of the recombinant protein into rice leaves caused disease‐like symptoms. In the present study, we further characterized the functionality of RsIA_CtaG/Cox11. RsIA_CtaG/Cox11 is an alternative transcript of cytochrome c oxidase copper chaperone Cox11 that starts from the second AUG codon, but contains a functional secretion signal peptide. RNA interference with RsIA_CtaG/Cox11 reduced the pathogenicity of AG‐1 IA towards rice and Nicotiana benthamiana without affecting its fitness or mycelial morphology. Transient expression of the RsIA_CtaG/Cox11‐GFP fusion protein demonstrated the localization of RsIA_CtaG/Cox11 to mitochondria. Agro‐infiltration of RsIA_CtaG/Cox11 into N. benthamiana leaves inhibited cell death by BAX and INF1. In contrast to rice, agro‐infiltration of RsIA_CtaG/Cox11 did not induce cell death in N. benthamiana. However, cell death was observed when it was coinfiltrated with Os_CoxVIIa, which encodes a subunit of cytochrome c oxidase. Os_CoxVIIa appeared to interact with RsIA_CtaG/Cox11. The cell death triggered by coexpression of RsIA_CtaG/Cox11 and Os_CoxVIIa is independent of the leucine‐rich repeat receptor kinases BAK1/SOBIR1 and enhanced the susceptibility of N. benthamiana to AG‐1 IA. Two of the three evolutionarily conserved cysteine residues at positions 25 and 126 of RsIA_CtaG/Cox11 were essential for its immunosuppressive activity, but not for cell death induction. This report suggests that RsIA_CtaG/Cox11 appears to have a dual role in immunosuppression and cell death induction during pathogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitochondrial‐targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG‐1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit

Zhang,

Lin,

Yamamoto

et al. 2023

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Based on bioinformatics analysis, we determined the CaS gene to be located in the nucleus chromosome, which possessed a chloroplast transit peptide, indicating that CaS transcription occurred in nuclear, processed from Endoplasmic reticulum to Golgi apparatus, and eventually ended up in the chloroplast. Chloroplasts play an important role in the defense response plants and are a common target of the phytopathogen effectors [ 44 ]. HopI1 located in chloroplasts from P. syringae suppresses SA accumulation and leads to the reconstruction of the thylakoid structure [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas syringae pv. actinidiae Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity

Zhang

Zhou

Liu

et al. 2022

IJMS

View full text Add to dashboard Cite

Kiwifruit canker, caused by Pseudomonas syringae pv. actinidiae (Psa), is a destructive pathogen that globally threatens the kiwifruit industry. Understanding the molecular mechanism of plant-pathogen interaction can accelerate applying resistance breeding and controlling plant diseases. All known effectors secreted by pathogens play an important role in plant-pathogen interaction. However, the effectors in Psa and their function mechanism remain largely unclear. Here, we successfully identified a T3SS effector HopAU1 which had no virulence contribution to Psa, but could, however, induce cell death and activate a series of immune responses by agroinfiltration in Nicotiana benthamiana, including elevated transcripts of immune-related genes, accumulation of reactive oxygen species (ROS), and callose deposition. We found that HopAU1 interacted with a calcium sensing receptor in N. benthamiana (NbCaS) as well as its close homologue in kiwifruit (AcCaS). More importantly, silencing CaS by RNAi in N. benthamiana greatly attenuated HopAU1-triggered cell death, suggesting CaS is a crucial component for HopAU1 detection. Further researches showed that overexpression of NbCaS in N. benthamiana significantly enhanced plant resistance against Sclerotinia sclerotiorum and Phytophthora capsici, indicating that CaS serves as a promising resistance-related gene for disease resistance breeding. We concluded that HopAU1 is an immune elicitor that targets CaS to trigger plant immunity.

show abstract

“…Although early fungal effectors (microbial proteins used by the microbe to manipulate the host metabolism and their fundamental processes) were described as small secreted, cysteine-rich, extracellular proteins [ 54 ], knowledge about these pathogenicity factors has changed recently. Currently, it is known that many effectors target nuclei or mitochondrial proteins in the hosts, instead of functioning in the extracellular space [ 55 , 56 ]. In addition, some effectors/pathogenicity factors which have no signal peptide are secreted through multivesicular bodies (which is called an “unconventional secretion system”), which become extracellular vesicles called “virulence bags” [ 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Gutiérrez-Domínguez

Chí-Manzanero

Rodríguez-Argüello

et al. 2022

IJMS

View full text Add to dashboard Cite

Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of Bacillus, Paucimonas lemoignei, and the yeast Trichosporon asahii; they are usually thermotolerant and solvent resistant. Recently, while searching for true lipases in the Trichoderma harzianum genome, one lipase containing the pentapeptide AHSMG was identified. In this study, we cloned from T. harzianum strain B13-1 the lipase ID135964, renamed here as ThaL, which is 97.65% identical with the reference. We found that ThaL is a lid-containing true lipase of cluster III that belongs to a large family comprising highly conserved proteins in filamentous fungi in the orders Hypocreales and Glomerellales, in which predominantly pathogenic fungi are found. ThaL was expressed in conidia, as well as in T. harzianum mycelium, where it was cultured in liquid minimal medium. These results—together with the amino acid composition, absence of a signal peptide, mitochondrial sorting prediction, disordered regions in the protein, and lineage-specific phylogenetic distribution of its homologs—suggest that ThaL is a non-canonical effector. In summary, AHSMG-lipase is a novel lipase family in filamentous fungi, and is probably involved in pathogenicity.

show abstract

Plant mitochondria and chloroplasts are targeted by the Rhizoctonia solani RsCRP1 effector

Cited by 17 publications

References 34 publications

Mitochondrial‐targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG‐1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit

Mitochondrial‐targeting effector RsIA_CtaG/Cox11 in Rhizoctonia solani AG‐1 IA has two functions: plant immunity suppression and cell death induction mediated by a rice cytochrome c oxidase subunit

Pseudomonas syringae pv. actinidiae Effector HopAU1 Interacts with Calcium-Sensing Receptor to Activate Plant Immunity

Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi

Contact Info

Product

Resources

About