Plant myo-inositol transport influences bacterial colonization phenotypes

The Function of Root Exudates in the Root Colonization by Beneficial Soil Rhizobacteria

“…The most abundant inositol isomer present in root exudates is myo-inositol (referred to as inositol in this review) [15]. Recent studies have revealed that inositol can act as a carbon source to promote bacterial growth and as a signaling molecule to enhance various processes, including chemotaxis, biofilm formation, siderophore production, and colonization by beneficial plant-associated bacteria [90][91][92]. In Arabidopsis, inositol transporters, such as INT1 and PMT5, are necessary for the secretion of inositol by roots, which mediates the degree of microbial root colonization [92].…”

“…Recent studies have revealed that inositol can act as a carbon source to promote bacterial growth and as a signaling molecule to enhance various processes, including chemotaxis, biofilm formation, siderophore production, and colonization by beneficial plant-associated bacteria [90][91][92]. In Arabidopsis, inositol transporters, such as INT1 and PMT5, are necessary for the secretion of inositol by roots, which mediates the degree of microbial root colonization [92]. DNA methylation is an epigenetic marker in plants that occurs in response to various environmental stimuli and is important for various fundamental biological processes [110].…”

“…Bacterial inositol catabolism (iol locus) enhances bacterial competence in natural soil and is associated with greater root colonization. For example, recent studies have shown that inositol can enhance swimming motility and growth and alter the colony morphology of Pseudomonas and Pantoea strains via inositol catabolism [91,92]. In Pseudomonas strains, the inositol-induced repression of DksA, a transcriptional regulator involved in inhibiting swimming motility, has been reported as a potential mechanism for inositol-triggered swimming motility [92].…”

MENTOR: Multiplex Embedding of Networks for Team-Based Omics Research

“…RNA-seq data from xylem tissues of a diverse population of P. trichocarpa genotypes were obtained and reads were aligned, trimmed, and filtered against the P. trichocarpa v3.0 reference genome from Phytozome [16][17][18] . Reads that did not map to the reference genome were considered putative microbiome members and included in subsequent metatranscriptomic analyses.…”

“…Significant SNPs (FDR-adjusted p-value ≤ 0.1) across all models for both Stagonospora and Ilyonectria were assigned to the closest gene using a modified version of bedtools --closest function 31 and the poplar v3.1 .gff file obtained from Phytozome 17 . We combined the genes identified across all models for each fungal taxa as the input gene set and used a previously described P. trichocarpa multiplex 18 as input to MENTOR. The genes in the dendrogram output were divided across team members for interpretation in the context of host-microbial interactions.…”