Plant–necrotroph co-transcriptome networks illuminate a metabolic battlefield

Abstract

A central goal of studying host-pathogen interaction is to understand how host and pathogen manipulate each other to promote their own fitness in a pathosystem. Co-transcriptomic approaches can simultaneously analyze dual transcriptomes during infection and provide a systematic map of the cross-kingdom communication between two species. Here we used the Arabidopsis-B. cinerea pathosystem to test how plant host and fungal pathogen interact at the transcriptomic level. We assessed the impact of genetic diversity in pathogen and host by utilization of a collection of 96 isolates infection on Arabidopsis wild-type and two mutants with jasmonate or salicylic acid compromised immunities. We identified ten B. cinereagene co-expression networks (GCNs) that encode known or novel virulence mechanisms. Construction of a dual interaction network by combining four host- and ten pathogen-GCNs revealed potential connections between the fungal and plant GCNs. These co-transcriptome data shed lights on the potential mechanisms underlying host-pathogen interaction.

Fight hard or die trying: when plants face pathogens under heat stress

“…The approaches listed above are all plant centred and do not give access to the pathogen transcriptome. Solutions could come from dual‐transcriptome analyses, as recently used to study plant–pathogen interactions (Zhang et al ., 2019). In addition to providing valuable information on the gene networks that control cross‐kingdoms interactions, they would allow the identification of factors that regulate pathogen fitness and virulence under heat stress during the interaction.…”

Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in the Phytopathogenic Fungus Botrytis cinerea

“…Involved in the Pathogenicity of B. cinerea B. cinerea is one of the most destructive plant pathogens, can infect more than 200 plants and is thus a model generalist pathogen for studying the interactions between plant hosts and fungal pathogens (Soltis et al, 2019;Xiong et al, 2019). To infect hosts successfully, pathogens, such as B. cinerea, employs multiple strategies based on quantitative genetic architectures, including numerous extracellular enzymes, proteins, metabolite and battling with hosts in metabolic levels (Kliebenstein et al, 2005;Nakajima and Akutsu, 2014;Corwin and Kliebenstein, 2017;Zhang et al, 2019). In recent years, epigenetic regulation was also reported to be involved in the regulation of pathogenicity of pathogens (Dubey and Jeon, 2017;Izbiańska et al, 2019).…”

Quantitative interactions driveBotrytis cinereadisease outcome across the plant kingdom

“…While the gene-for-gene model works well for qualitative host-specialist pathogen interactions, a genome-for-genome model is required to apprehend the dynamic and resilience of generalist pathogens interactions. The Botrytis-Arabidopsis pathosystem recently highlighted such genome-for-genome interactions with metabolic battle between host and pathogen (Zhang et al, 2019, Zhang et al, 2017.…”

“…To respond to pathogen attacks, plants use resistance mechanisms composed of innate and inducible immune systems that recognize danger and mount physiological, physical and chemical responses (Jones and Dangl, 2006). To counter the plant defenses, pathogens use diverse mechanisms to attack and/or interfere with the host including virulence factors and toxins (Rodriguez-Moreno et al, 2018, Zhang et al, 2019. This dynamic co-evolution of plants and pathogens is shaped and altered by the interaction of the genetic diversity in both the host resistance and the pathogen virulence genes (Karasov et al, 2014, Gilbert and Parker, 2016, Stam and McDonald, 2018.…”