Plant nucleolar DNA: Green light shed on the role of Nucleolin in genome organization

Picart, Catherine; Pontvianne, Frédéric

doi:10.1080/19491034.2016.1236167

Cited by 16 publications

(17 citation statements)

References 29 publications

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“…In parallel, inactive hubs, corresponding to B compartments, were shown to associate with the nucleolus [1]. This corroborates previous observations made in both animal and plant cells [25,38], regarding the important role of the nucleolus in organizing inactive chromatin regions at the nucleolar periphery.…”

Section: Influence Of Rna Genes In Nucleolus-associated Chromatin Domsupporting

confidence: 90%

“…Majority of NADs are presumed to be located at the nucleolus periphery, but cytological analyses demonstrated that the DNA inside the nucleolus is not only composed of rRNA genes [24]. Since RNA polymerase II is absent from inside the nucleolus, it has been proposed that the nucleolus would have a negative impact on gene expression [25,26].…”

Section: The Nucleolus a Platform For Genome Organizationmentioning

confidence: 99%

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Ribosomal RNA genes shape chromatin domains associating with the nucleolus

Picart-Picolo

Picault

Pontvianne

2019

Nucleus

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Genomic interactions can occur in addition to those within chromosome territories and can be organized around nuclear bodies. Several studies revealed how the nucleolus anchors higher order chromatin structures of specific chromosome regions displaying heterochromatic features. In this review, we comment on advances in this emerging field, with a particular focus on a recent study published by Quinodoz et al., that developed a new method to characterize simultaneous genomic interactions in the same cell. Highlighting studies conducted in animal and plant cells, we then discuss the establishment of inactive chromatin at nucleolus organizer region (NOR)bearing chromosomes.

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Section: Influence Of Rna Genes In Nucleolus-associated Chromatin Domsupporting

confidence: 90%

Section: The Nucleolus a Platform For Genome Organizationmentioning

confidence: 99%

Ribosomal RNA genes shape chromatin domains associating with the nucleolus

Picart-Picolo

Picault

Pontvianne

2019

Nucleus

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“…Fibrillarin is a nucleolar protein known to localize to the dense fibrillar component, forming ring-like structures in the nucleolus (Picart and Pontvianne, 2016) and can be used as a marker of nucleolus integrity (Pontvianne et al, 2013). Using FIB2-YFP stable line, the nucleolus status was checked under normal and HS conditions.…”

Section: Polysome Recovery Can Be Independent Of Transcription Restartmentioning

confidence: 99%

Heat Shock Protein HSP101 Affects the Release of Ribosomal Protein mRNAs for Recovery after Heat Shock

Merret

Carpentier²,

Favory³

et al. 2017

Plant Physiol.

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Heat shock (HS) is known to have a profound impact on gene expression at different levels, such as inhibition of protein synthesis, in which HS blocks translation initiation and induces the sequestration of mRNAs into stress granules (SGs) or P-bodies for storage and/or decay. SGs prevent the degradation of the stored mRNAs, which can be reengaged into translation in the recovery period. However, little is known on the mRNAs stored during the stress, how these mRNAs are released from SGs afterward, and what the functional importance is of this process. In this work, we report that Arabidopsis HEAT SHOCK PROTEIN101 (HSP101) knockout mutant (hsp101) presented a defect in translation recovery and SG dissociation after HS. Using RNA sequencing and RNA immunoprecipitation approaches, we show that mRNAs encoding ribosomal proteins (RPs) were preferentially stored during HS and that these mRNAs were released and translated in an HSP101-dependent manner during recovery. By 15 N incorporation and polysome profile analyses, we observed that these released mRNAs contributed to the production of new ribosomes to enhance translation. We propose that, after HS, HSP101 is required for the efficient release of RP mRNAs from SGs resulting in a rapid restoration of the translation machinery by producing new RPs.

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“…It seems that the terminal position has at least one advantage: it is the only chromosomal position that allows the occurrence of non-homologous recombination, necessary for interlocus homogenization of rDNA repeats, without disturb the linkage of other genes (Pedrosa-Harand et al, 2006). Moreover, nucleolus-related telomeric and subtelomeric sequences contribute to the regulation of rDNA gene transcription by RNA polymerase II and to the stability of the nucleolus (Pontvianne et al, 2016;Picart and Pontvianne, 2017). However, if the terminal position of 35S rDNA sites is condition that is positively selected, why Ap sites, which are at the same time proximally and terminally located, are more common and more frequently activated in Nothoscordum than in other terminal sites?…”

Section: Discussionmentioning

confidence: 99%

Does the chromosomal position of 35S rDNA sites influence their transcription? A survey on Nothoscordum species (Amaryllidaceae)

2020

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35S ribosomal DNA (rDNA) sites are the regions where the ribosomal genes 18S, 5.8S and 25S, responsible for the formation of the nucleoli, are found. The fact that rDNA sites have non-random distribution on chromosomes suggests that their positions may influence their transcription. To identify if the preferentially transcribed rDNA sites occupy specific position, six species (nine cytotypes) of the genus Nothoscordum were analyzed using two different techniques to impregnate the nucleolar organizer regions (NORs) with silver nitrate. Both techniques strongly stained NORs, but one of them also stained the proximal region of all chromosomes, suggesting the existence of another group of argentophilic proteins in this region. In species with rDNA sites in acrocentric and metacentric chromosomes, sites located on the short arms of the acrocentric chromosomes were preferentially activated. On the other hand, in species with rDNA sites restricted to the short arms of the acrocentrics, all of them were activated, whereas in those species with sites restricted to the terminal region of metacentric chromosomes, the frequency of active sites was always lower than expected. This indicate that, at least in Nothoscordum, the transcription of an rDNA site is influenced by its chromosomal position, and may explain, at least partially, the strongly non-random distribution of these sites in plant and animal chromosomes.

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Plant nucleolar DNA: Green light shed on the role of Nucleolin in genome organization

Cited by 16 publications

References 29 publications

Ribosomal RNA genes shape chromatin domains associating with the nucleolus

Ribosomal RNA genes shape chromatin domains associating with the nucleolus

Heat Shock Protein HSP101 Affects the Release of Ribosomal Protein mRNAs for Recovery after Heat Shock

Does the chromosomal position of 35S rDNA sites influence their transcription? A survey on Nothoscordum species (Amaryllidaceae)

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