Plant nutritional status modulates glutamine synthetase levels in ripe tomatoes (Solanum lycopersicum cv. Micro-Tom)

Scarpeci, Telma E.; Marro, M.; Bortolotti, Santiago; Boggio, Silvana B.; Valle, Estela M.

doi:10.1016/j.jplph.2006.01.003

Cited by 35 publications

(27 citation statements)

References 28 publications

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“…Further, GS expression and activity was increased by ammonium and nitrate and also by cadmium and salt stress, but decreased by copper, aluminum, drought, cold, and heat stress [37]. Differential expression of GS in different organs and modulation in cytosolic GS expression upon supplemented nutritional regime and salt stress has also been shown in tomato [38,39]. Induction in the expression of cytosolic GS by SA has been reported recently in an only report on tobacco [40].…”

Section: Inducibility Of Csgs Transcript Expression As Well As Activimentioning

confidence: 74%

Cloning and Characterization of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze that is Upregulated by ABA, SA, and H2O2

2007

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A cDNA encoding glutamine synthetase, one of the enzymes of the GS/GOGAT pathway, was cloned from Camellia sinensis (CsGS). The isolated cDNA consists of 1,071 nucleotides encoding a polypeptide of 356 amino acids with an estimated isoelectric point of 6.13. The recombinant protein purified from Escherichia coli using Ni-NTA affinity chromatography showed molecular mass of 39.2 kDa. The purified protein was confirmed by blotting with anti-His antibodies. Catalytic parameters of the protein were determined using glutamate and ATP as substrates. The observed Km was 9 mM and Vmax was 93 U/mg protein with glutamate as substrate, while with ATP Km and Vmax values were 6 mM and 70 U/mg protein, respectively. Purified enzyme showed pH optima at 8. Cations were found to be showing enhancing effect on the activity of GS enzyme and Mg2+ ion exhibited maximum enhancing effect among the various ions used in this study. This enzyme activity increased by 25% in presence of DTT and decreased by 18% when incubated with PMSF. Transcript analysis in tea bud, youngest leaf, showed that CsGS gene expression is stimulated in response to abscisic acid (ABA), salicylic acid (SA), and hydrogen peroxide (H2O2), while gibberellic acid (GA3) has no influence on its expression levels.

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Section: Inducibility Of Csgs Transcript Expression As Well As Activimentioning

confidence: 74%

Cloning and Characterization of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze that is Upregulated by ABA, SA, and H2O2

2007

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“…This multi-stress response of GS suggests the presence of stress responsive elements in the promoter region of the GS gene. Although differential expression of GS has been shown in different organs like root, leaf, and flower in tomato (Scarpeci et al 2007), this upregulation of the GS reported in response to salinity and cadmium stress is a novel observation.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 79%

Expression of tea cytosolic glutamine synthetase is tissue specific and induced by cadmium and salt stress

Rana¹,

Mohanpuria²,

Yadav³

2008

Biologia plant.

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Glutamine synthetase (GS) showed highest expression and activity in bud (youngest topmost leaf) of Camellia sinensis, lower in older leaves, while lowest activity in stem and roots. GS expression and activity was increased by ammonium and nitrate and also by cadmium and salt stress but decreased by copper, aluminum, drought, cold and heat stress.Additional key words: abiotic stress, Camellia sinensis, metal stress, N-source.

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“…The homogenates were clarified by centrifugation, and the supernatants were separated by 10% SDS-PAGE (Laemmli, 1970). Proteins were electroblotted onto polyvinylidene difluoride membrane (Bio-Rad) and detected using specific polyclonal antibodies against TSS, GS (Scarpeci et al, 2007), chloroplastic Hsp70, 2-Cys Prx, and chloroplastic CuZnSOD. Bound antibodies were located by linking to alkaline phosphatase-conjugated goat anti-rabbit IgG according to the manufacturer's instructions (Sigma-Aldrich).…”

Section: Protein Detection By Western-blot Analysismentioning

confidence: 99%

Generation of Hydrogen Peroxide in Chloroplasts of Arabidopsis Overexpressing Glycolate Oxidase as an Inducible System to Study Oxidative Stress

et al. 2008

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Arabidopsis (Arabidopsis thaliana) overexpressing glycolate oxidase (GO) Moreover, the data demonstrate that a response to oxidative stress is installed, with increased expression and/or activity of known oxidative stress-responsive components. Hence, the GO plants are an ideal noninvasive model system in which to study the effects of H 2 O 2 directly in the chloroplasts, because H 2 O 2 accumulation is inducible and sustained perturbations can reproducibly be provoked by exposing the plants to different ambient conditions.

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Plant nutritional status modulates glutamine synthetase levels in ripe tomatoes (Solanum lycopersicum cv. Micro-Tom)

Cited by 35 publications

References 28 publications

Cloning and Characterization of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze that is Upregulated by ABA, SA, and H2O2

Cloning and Characterization of a Cytosolic Glutamine Synthetase from Camellia sinensis (L.) O. Kuntze that is Upregulated by ABA, SA, and H2O2

Expression of tea cytosolic glutamine synthetase is tissue specific and induced by cadmium and salt stress

Generation of Hydrogen Peroxide in Chloroplasts of Arabidopsis Overexpressing Glycolate Oxidase as an Inducible System to Study Oxidative Stress

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