Plant oils as promising substrates for polyhydroxyalkanoates production

Ciesielski, Sławomir; Możejko, Justyna; Pisutpaisal, Nipon

doi:10.1016/j.jclepro.2014.09.040

Cited by 87 publications

(40 citation statements)

References 105 publications

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“…The composition of the PHAs synthesized by this mutant was similar to composition of mcl-PHAs produced by other Pseudomonas species cultivated on fatty acids (Ciesielski and Mozejko 2015). …”

Section: Resultssupporting

confidence: 72%

Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

et al. 2017

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Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli are still not clear. The rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes in order to promote survival under nutritional deprivation conditions. Therefore, in this work we investigated the relation between mcl-PHAs synthesis and stringent response. For this study, a relA/spoT mutant of P. putida KT2440, unable to induce the stringent response, was used. Additionally, the transcriptome of this mutant was analyzed using RNA-seq in order to examine rearrangements of the metabolism during cultivation. The results show that the relA/spoT mutant of P. putida KT2440 is able to accumulate mcl-PHAs in both optimal and nitrogen limiting conditions. Nitrogen starvation did not change the efficiency of mcl-PHAs synthesis in this mutant. The transition from exponential growth to stationary phase caused significant upregulation of genes involved in transport system and nitrogen metabolism. Transcriptional regulators, including rpoS, rpoN and rpoD, did not show changes in transcript abundance when entering the stationary phase, suggesting their limited role in mcl-PHAs accumulation during stationary phase.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0396-z) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 72%

Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

et al. 2017

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“…3-hydroxytetradecanoate was found in low amounts in mcl-PHAs obtained from P. putida KT2440 cultivated on sodium gluconate (Table 1). The major repeat units were similar to those produced by other Pseudomonas species cultivated on related and non-related carbon sources [9, 10]. …”

Section: Resultssupporting

confidence: 57%

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

Możejko-Ciesielska

Pokój

Ciesielski

2018

Journal of Industrial Microbiology and Biotechnology

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Bacterial response to environmental stimuli is essential for survival. In response to fluctuating environmental conditions, the physiological status of bacteria can change due to the actions of transcriptional regulatory machinery. The synthesis and accumulation of polyhydroxyalkanoates (PHAs) are one of the survival strategies in harsh environments. In this study, we used transcriptome analysis of Pseudomonas putida KT2440 to gain a genome-wide view of the mechanisms of environmental-friendly biopolymers accumulation under nitrogen-limiting conditions during conversion of metabolically different carbon sources (sodium gluconate and oleic acid). Transcriptomic data revealed that phaG expression is associated with medium-chain-length-PHAs’ synthesis not only on sodium gluconate but also on oleic acid, suggesting that PhaG may play a role in this process, as well. Moreover, genes involved in the β-oxidation pathway were induced in the PHAs production phase when sodium gluconate was supplied as the only carbon and energy source. The transition from exponential growth to stationary phase caused a significant expression of genes involved in nitrogen metabolism, energy supply, and transport system. In this study, several molecular mechanisms, which drive mcl-PHAs synthesis, have been investigated. The identified genes may provide valuable information to improve the efficiency of this bioprocess and make it more economically feasible.Electronic supplementary materialThe online version of this article (10.1007/s10295-018-2042-4) contains supplementary material, which is available to authorized users.

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“…Over the last decades, the adoption of these crops has been growing up significantly, reaching in 2014 more than 300 million hectares of oil crops worldwide cultivated [ Figure 1] [3]. The main reason for this growth is due to seed oils are so attractive for industries, as mentioned in the previous paragraph, but also for the possibility to use their sub-products (metabolites) in biofuels development [4] and approaching their polyhydroxyalkanoates (PHAs) in response to petrol-based plastics waste's problems and harmful effects on the environment [5]. …”

Section: Oilseed Cropsmentioning

confidence: 99%

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

Villanueva-Mejía¹,

Álvarez²

2017

Advances in Seed Biology

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In 2009, big challenges facing the agricultural sector in the twenty-first century were presented to the world. Human population growth, increased life expectancy, loss of biodiversity, climate change and accelerated land degradation are the main factors contributing to rethink agriculture system production. In that scenery, modern biotechnology has set a stage for the advancement of agricultural practices and it is clearly an important ally to apply a broad array of technologies and innovative systems where they are most needed, such as enhancing crop productivity, increasing yields, and ultimately ensuring food security. One of the biggest challenges is related to technify production systems, but with no doubt, developing genetic improvement toward getting an efficient and sustainable agriculture, generating new seed qualities (new traits), such as, among others, to upset fatty acids content in oilseed crops have been growing up significantly due to industry interest. In this study, a review about the main advances in genetic improvement of some oilseed crops, starting with omics to understand metabolic routes and to find out key genes in seed oil production, and also, getting in use of modern biotechnology to alter the production of fatty acids, and to face biotic challenges in oilseed crops is presented.

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Plant oils as promising substrates for polyhydroxyalkanoates production

Cited by 87 publications

References 105 publications

Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

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