Plant origin authentication of Sonoran Desert propolis: an antiproliferative propolis from a semi-arid region

Alday, Efrain; Valencia, Dora; Garibay‐Escobar, Adriana; Domínguez-Esquivel, Zaira; Piccinelli, Anna Lisa; Rastrelli, Luca; Monribot‐Villanueva, Juan L.; Guerrero‐Analco, José A.; Robles‐Zepeda, Ramón Enrique; Hernández, Javier; Velázquez, Carlos

doi:10.1007/s00114-019-1620-2

Cited by 26 publications

(30 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In particular, the development of seasonal studies when properly delineated has great advantages and can contribute with the establishment of the chemical composition of the commercial propolis 18,19 . It establishes the best times of the year for the harvesting of propolis with excellent quality 20 ; it avoids the falsification or the purchase of propolis raw material in low quality 21 and it establishes indicators minimum quality from commercial point view 21–23 . In research, the seasonality can assists and facilitates phytochemical research to identify interest chemical compounds during the discovery of medicinal substances when coupled with universal detector during fingerprint studies 23 .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Nascimento

Arruda

Almeida

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world’s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC50 from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.

show abstract

Section: Introductionmentioning

confidence: 99%

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Nascimento

Arruda

Almeida

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Caborca propolis (CP) was collected over the four seasons of the year, from winter 2014 to fall 2015. Previous studies have shown the chemical stability of Sonoran propolis collected throughout the years [ 19 , 20 , 36 ]. Other studies have reported no qualitative changes in the chemical composition of propolis after several years of storage at freezing temperatures [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…The seasonal samples of CP (10 g) were cut into small pieces and extracted with methanol (50 mL) for three days, then filtered to concentrate the methanolic extracts under reduced pressure. Once the methanol was evaporated from the propolis methanolic extract, we removed the remaining waxes in CP by washing the obtained dry extracts with hexane (1:3 p/v), as previously published by our research group [ 19 , 20 , 36 ]. Seasonal samples of CP were stored in the dark at −20 °C until analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The broad array of biological activities of propolis include antioxidant, antibacterial, antiparasitic, antifungic, immunomodulatory activity and antiproliferative effects, among others [ 10 , 12 , 13 , 14 , 15 , 16 , 17 ]. At present, the seasonal influence on propolis production from dissimilar geographical areas, as well as derived from different plant origins, has been studied to establish a correlation between its pharmacological potential and the qualitative and quantitative fluctuations of propolis constituents throughout the year [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis

et al. 2020

Self Cite

View full text Add to dashboard Cite

The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC50: 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC50: 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC50: 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H2O2 in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H2O2 (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time.

show abstract

“…Investigações sobre esta resina em regiões tropicais revelaram que, em muitos casos, os flavonoides são os principais componentes destas amostras, similarmente às Europeias, embora a sua origem vegetal seja diferente. [18][19] Por exemplo, em amostras do deserto de Sonora, alguns autores identificaram flavonoides aglicônicos típicos de exsudatos da planta Ambrosia deltoidea: 3,5,7-tri-idroxi-3,8-dimetoxiflavona, xantomicrol, hispidulina, sideritiflavona, kaempferol, quercetina, ramnazina e outros componentes em menor concentração. Miricetina 3,7,4',5'-tetrametil éter e quercetina 3,7,3'-trimetil éter foram isolados da própolis da Tunísia, originando-se de exsudatos de folhas de algumas espécies de Citrus.…”

Section: Introductionunclassified

Accessible Methodologies for Quantification of Flavonoids and Total Phenols in Propolis

Marcucci¹,

Salatino²,

Oliveira³

et al. 2021

Rev. Virtual Quim.

View full text Add to dashboard Cite

Propolis is widely known for its important therapeutic activities. In its chemical constitution, there are several classes of secondary metabolites, such as simple phenols, carboxylic and cinnamic acids, coumarins, tannins and flavonoids. However, flavonoids, an important class of secondary metabolites, may be found in small concentrations in propolis, which makes its quantification difficult. In view of this information, the purpose of this review is to present the main methodologies used in the quantification of this class of secondary metabolites, in addition to proposing new approaches for the characterization of flavonoids and phenols, using low-cost commercial raw materials. A própolis é largamente conhecida por suas importantes atividades terapêuticas. Apresenta em sua constituição química diversas classes de metabólitos secundários, tais como, fenóis simples, ácidos carboxílicos e cinâmicos, cumarinas, taninos e flavonoides. Entretanto, os flavonoides, uma classe importante de metabólicos secundários, muitas vezes encontra-se em pequenas concentrações na própolis, o que pode dificultar sua quantificação. Tendo em vista estas informações, esta revisão teve como objetivo relacionar as principais metodologias utilizadas na identificação e quantificação desta classe de metabólitos secundários, além de propor novas abordagens para a caracterização de flavonoides e fenóis totais, a partir da utilização de matérias primas comerciais de baixo custo.

show abstract

Plant origin authentication of Sonoran Desert propolis: an antiproliferative propolis from a semi-arid region

Cited by 26 publications

References 38 publications

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Seasonality Modulates the Cellular Antioxidant Activity and Antiproliferative Effect of Sonoran Desert Propolis

Accessible Methodologies for Quantification of Flavonoids and Total Phenols in Propolis

Contact Info

Product

Resources

About