Plant Photosynthetic Pigments: Methods and Tricks for Correct Quantification and Identification

Fernández‐Marín, Beatriz; Garcı́a-Plazaola, José Ignacio; Hernández, A.; Esteban, Raquel

doi:10.1007/978-3-319-93233-0_3

Cited by 12 publications

(8 citation statements)

References 61 publications

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“…Composition of R. myconi leaves was characterised by a relatively high Chl content and low Chl a : b ratio (Fig. 2) but within an expected range for a shade‐preferring species (Sárvári et al , 2014; Esteban et al , 2015; Georgieva et al , 2017b; Fernández‐Marín et al , 2018a). The VAZ pool and the Toc : Chl ratios were moderately high and comparable to values of Mediterranean herbaceous species (Munné‐Bosch et al , 2016; Fernández‐Marín et al , 2017).…”

Section: Discussionmentioning

confidence: 69%

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

et al. 2020

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Summary Resurrection plants recover physiological functions after complete desiccation. Almost all of them are native to tropical warm environments. However, the Gesneriaceae include four genera, remnant of the past palaeotropical flora, which inhabit temperate mountains. One of these species is additionally freezing‐tolerant: Ramonda myconi. We hypothesise that this species has been able to persist in a colder climate thanks to some resurrection‐linked traits. To disentangle the physiological mechanisms underpinning multistress tolerance to desiccation and freezing, we conducted an exhaustive seasonal assessment of photosynthesis (gas exchange, limitations to partitioning, photochemistry and galactolipids) and primary metabolism (through metabolomics) in two natural populations at different elevations. R. myconi displayed low rates of photosynthesis, largely due to mesophyll limitation. However, plants were photosynthetically active throughout the year, excluding a reversible desiccation period. Common responses to desiccation and low temperature involved chloroplast protection: enhanced thermal energy dissipation, higher carotenoid to Chl ratio and de‐epoxidation of the xanthophyll cycle. As specific responses, antioxidants and secondary metabolic routes rose upon desiccation, while putrescine, proline and a variety of sugars rose in winter. The data suggest conserved mechanisms to cope with photo‐oxidation during desiccation and cold events, while additional metabolic mechanisms may have evolved as specific adaptations to cold during recent glaciations.

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Section: Discussionmentioning

confidence: 69%

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

et al. 2020

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“…Chlorophyll a and b are present throughout the photosynthetic machinery whereas carotenoids like β-carotene, violaxanthin, antheraxanthin, zeaxanthin and lutein are mainly present in antenna systems of light harvesting complexes [ 15 , 16 ]. During periods of high-light stress, plants adopt to the phenomenon of photoinhibition leading to reduced levels of light capture.…”

Section: Pigments In Fruitsmentioning

confidence: 99%

Fruit ripening: dynamics and integrated analysis of carotenoids and anthocyanins

et al. 2022

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Background Fruits are vital food resources as they are loaded with bioactive compounds varying with different stages of ripening. As the fruit ripens, a dynamic color change is observed from green to yellow to red due to the biosynthesis of pigments like chlorophyll, carotenoids, and anthocyanins. Apart from making the fruit attractive and being a visual indicator of the ripening status, pigments add value to a ripened fruit by making them a source of nutraceuticals and industrial products. As the fruit matures, it undergoes biochemical changes which alter the pigment composition of fruits. Results The synthesis, degradation and retention pathways of fruit pigments are mediated by hormonal, genetic, and environmental factors. Manipulation of the underlying regulatory mechanisms during fruit ripening suggests ways to enhance the desired pigments in fruits by biotechnological interventions. Here we report, in-depth insight into the dynamics of a pigment change in ripening and the regulatory mechanisms in action. Conclusions This review emphasizes the role of pigments as an asset to a ripened fruit as they augment the nutritive value, antioxidant levels and the net carbon gain of fruits; pigments are a source for fruit biofortification have tremendous industrial value along with being a tool to predict the harvest. This report will be of great utility to the harvesters, traders, consumers, and natural product divisions to extract the leading nutraceutical and industrial potential of preferred pigments biosynthesized at different fruit ripening stages.

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“…The collected leaves were split in half, with one part stored in the dark at room temperature (20°C) and the other part placed in a freezer (−20°C) to prevent pigment degradation [ 16 , 17 ]. The samples obtained from these leaves were named Group 1 (G1) and Group 2 (G2), respectively.…”

Section: Methodsmentioning

confidence: 99%

Enhanced pigment content estimation using the Gauss-peak spectra method with thin-layer chromatography for a novel source of natural colorants

Twardowska

2021

PLoS ONE

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Alternative pigment sources that are harmless to human health and can be produced in an eco-responsible way are of great research interest. The experiments undertaken in this study were conducted using autumn leaves of Aesculus hippocastanum as potential novel colorant sources. This study focused on improving the Gauss-peak spectra method (a less expensive alternative to high-pressure liquid chromatography) in combination with thin-layer chromatography, leading to the development of a new methodology. The collected leaves were stored at two different temperatures: 20°C and −20°C. The data obtained by spectrophotometric scanning of the samples were analyzed using the Gauss-peak spectra method in the R program with three wavelength ranges: 350–750 nm, 390–710 nm, and 400–700 nm. The results were then assessed for statistically significant differences in the estimated concentrations for the different wavelength ranges regarding (1) total pigment, carotenoid, and chlorophyll concentration (two-sample t-test) and (2) concentration of each indicated pigment (two-way analysis of variance). The results were also tested for differences between the estimated concentrations of samples stored under the different conditions. The Gauss-peak spectra results with and without thin-layer chromatography were statistically compared using a paired t-test. The results showed that thin-layer chromatography greatly enhanced the efficiency of the Gauss-peak spectra method for estimating the major and minor pigment composition without generating high additional costs. A wavelength range of 400–700 nm was optimal for all Gauss-peak spectra methods. In conclusion, the proposed method is a more successful, inexpensive alternative to high-pressure liquid chromatography.

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Plant Photosynthetic Pigments: Methods and Tricks for Correct Quantification and Identification

Cited by 12 publications

References 61 publications

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

Fruit ripening: dynamics and integrated analysis of carotenoids and anthocyanins

Enhanced pigment content estimation using the Gauss-peak spectra method with thin-layer chromatography for a novel source of natural colorants

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