Plant potential for detoxification (Review)

Zaalishvili, G.; Khatisashvili, Gia; Ugrkhelidze, D. Sh.; Gordeziani, M.; Kvesitadze, G.

doi:10.1007/bf02731887

Cited by 27 publications

(4 citation statements)

References 75 publications

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“…It is well known that glycosylation by plant cells serves as the detoxification step for toxic phenolic compounds, which could arise either from normal plant metabolism or from the environment [18,19]. The results above also suggested that exogenous aromatic compounds such as paeonol (1) and emodin (2) could act as chemical stress against the culture cells of P. quinquefolium.…”

Section: Discussionmentioning

confidence: 51%

Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium

L.¹,

Yan

Zhu³

et al. 2009

Appl Biochem Biotechnol

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Two aromatic substrates, paeonol (1) and emodin (2), were biotransformed by using transgenic crown galls of Panax quinquefolium. Four biotransformed products (3-6) were isolated and identified by physicochemical and spectral methods. A beta-glucoside (3, 73.2% of biotransformation yield) and a 1-(2,4-dimethoxyphenyl)- ethanone (4, 8.03%) were isolated from the suspension cultures after 7-day incubation of substrate 1. Upon administration of substrate 2, another beta-glucoside [emodin-6-O-beta-D: -glucopyranoside (5), 19.2%] and a hydroxylated derivative, citreorosein (6, 54.6%), were also obtained. The results demonstrate that transgenic crown galls of P. quinquefolium have the capacities to catalyze glycosylation, hydroxylation, and methylation reactions in the plant cells on those aromatic compounds.

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Section: Discussionmentioning

confidence: 51%

Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium

L.¹,

Yan

Zhu³

et al. 2009

Appl Biochem Biotechnol

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“…This is corroborated by the significant decrease of nitrobenzene oxidation rate in the presence of tiron (a superoxide radical scavenger) ( Table 3). The maximum inhibition of the process occurring during the simultaneous action of NaN 3 and tiron points to nitrobenzene oxidation occurring by both peroxidase and radical mechanisms.…”

Section: Resultsmentioning

confidence: 99%

“…It has been observed they can accomplish the reaction via a cooxidation pathway, e.g. phenoloxidase oxidizes the endogenous diphenol and the resultant quinone (or semiquinone) hydroxylates exogenous benzene [2,3].…”

Section: Introductionmentioning

confidence: 99%

Nitrobenzene oxidizing enzymes in plant cells

Varazashvili¹,

Khatisashvili²,

Kurashvili³

et al. 2001

JBPC

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Nitrobenzene NADPH-, H 2 O 2 -and O 2 -dependent oxidation in soybean and maize subcellular fractions has been studied. It is demonstrated that in the presence of nicotinamide coenzyme, transformation via monooxygenase has decisive significance. H 2 O 2 -and O 2 -dependent transformation of nitrobenzene is accomplished via either enzymatic or non-enzymatic (radical) mechanisms which results in formation of m-and p-nitrophenols.

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“…Hydroxylation and glycosylation are supposed to be initial steps to biodegradation of toxicants in plants. [ 21 ]…”

Section: Discussionmentioning

confidence: 99%

Stereo and region-selective biosynthesis of two new dihydroartemisinic acid glycosides by suspension-cultured cells of Artemisia annua

Zhu

Zeng

Song³

et al. 2014

Phcog Mag

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Background:The system of plant-cultured cells is one of the optimal systems to investigate biosynthesis pathway and their bioactive intermediates.Objective:To study the biosynthesis of dihydroartemisinic acid (1) by suspension-cultured cells of Artemisia annua.Materials and Methods:Substrate (compound 1) was administered into the suspension-cultured cells of A. annua and co-cultured for 2 days. The methanol extract was separated on various column chromatography methods and the structures of two biosynthesis products were elucidated based on the analysis of 1H NMR, 13C NMR, 2D NMR, and ESI-MS. Time-course curve was also established. Furthermore, in vitro antitumor activities of compounds 1-3 against HepG2, K562, and A549 cell lines were evaluated by MTT assay.Results:Two new compounds were obtained, namely 3α-hydroxy-dihydroartemisinic acid-α-D-glucopyranosyl ester (2) and 15-hydroxy-cadin-4-en-12-oic acid-β-d-glucopyranosyl ester (3). The results demonstrated that the cultured cells of A. annua possessed the abilities to stereo-selective hydroxylate and region-selective glycosylate sesquiterpene compounds in a highly efficient manner. Inhibitory effects of compounds 1-3 on proliferation of HepG2, K562, and A549 cell lines in vitro were also investigated.Conclusion:Two new dihydroartemisinic acid glycosides were obtained by stereo- and region-selective biosynthesis with cultured cells of A. annua.

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Plant potential for detoxification (Review)

Cited by 27 publications

References 75 publications

Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium

Biotransformation of Paeonol and Emodin by Transgenic Crown Galls of Panax quinquefolium

Nitrobenzene oxidizing enzymes in plant cells

Stereo and region-selective biosynthesis of two new dihydroartemisinic acid glycosides by suspension-cultured cells of Artemisia annua

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