Plant production in pea (Pisum sativum L. cvs. Puget and Upton) from long-term callus with superficial meristems

Hussey, G.; Gunn, Helen

doi:10.1016/0304-4211(84)90217-7

Cited by 51 publications

(28 citation statements)

References 6 publications

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“…9). The obtained micropropagation results were not so satisfactory as other authors data (Franklin et al 2000;Griga et al 1986;Hussey and Gunn 1984;Jackson and Hobbs 1990;Mallick and Rashid 1989), the average multiplication ratio was around 2.0 in comparison to 6.0-30.0. Presumably, lower micropropagation rate could be attributed to the fact that shoots obtained from callus were somewhat less vigorous despite shoots grown from original plumules.…”

Section: Discussioncontrasting

confidence: 78%

“…6, 7). The half-strength MS medium was more effective, as in the earlier reports (Cardi et al 1991;Hussey and Gunn 1984;Kubaláková et al 1988;Özcan et al 1992), but also not sufficient. Plants rooted on this medium were in poor condition and they adapted for ex vitro conditions with difficulty.…”

Section: Discussionsupporting

confidence: 47%

“…Efficient callus formation was stimulated for all studied cultivars on media P0 and P1. The media were supplemented, respectively, with KIN and 2,4-D or BAP and NAA, following to other authors (Hussey and Gunn 1984;Kosturkova et al 1997;Natali and Cavallini 1987;Schroeder et al 1993).…”

Section: Discussionmentioning

confidence: 99%

“…In the most of reported protocols, regenerated shoots were rooted directly without any pre-conditioning phase (Griga et al 1986;Hussey and Gunn 1984;Kubaláková et al 1988;NadolskaOrczyk et al 1994;Natali and Cavallini 1987;Nauerby et al 1991;Nielsen et al 1991;Özcan et al 1992). Our results did not confirm such possibility because the most of multiplicated shoots was dying after transfer onto rooting media.…”

Section: Discussionmentioning

confidence: 99%

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Organogenesis and long-term micropropagatlon of Polish pea cultivars

et al. 2011

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The complete protocol for regeneration and long-term micropropagation of several Polish cultivars of pea (Pisum sativum L.) has been elaborated. The shoots were the most likely regenerated via de novo organogenesis. The adventitious buds formed in callus derived from cotyledons tissue adjacent to the axillary meristems of immature embryos. All cultivars calli regenerated several shoots per explant on the MS medium supplemented with B5 vitamins and 4.5 mgl -1 of BAP, however some differences in regeneration capacity among cultivars were observed. The plantlets were subsequently micropropagated with slightly higher efficiency and preserving a good viability over the long-term culture on a medium containing 2.0 mgl -1 than one with 4.5 mgl -1 of BAP. The additional step of the pre-conditioning culture of multiplicated shoots on a medium with very low BAP concentration i.e. 0.02 mgl -1 was applied and appeared to be beneficial before rooting in vitro or grafting. The modified MS-derived medium with the half-strength of MS macroelements but with the full original dose of calcium and supplemented with B5 vitamins and 1.0 mgl -1 of NAA was developed for effective rooting. The shoots were also sufficiently transferred into ex vitro conditions using grafting. The majority of the regenerated plants had adapted to in vivo conditions in a greenhouse and subsequently has set seeds. The presented protocol provides relatively efficient rate of de novo pea regeneration and would be useful for Agrobacterium-mediated transformation purposes.

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Organogenesis and long-term micropropagatlon of Polish pea cultivars

et al. 2011

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“…from immature leaflets (Mroginski and Kartha, 1981;Rubluo et al, 1984), from cotyledonary node (Jackson and Hobbs, 1990), from hypocotyls (Nielsen et al, 1991), from embryos (Kysely et * Corresponding author; fax 61-6-246-5000. 75 1 Natali and Cavallini, 1987;Tetu et al, 1990), from various organs of seedlings (Malmberg, 1979;Hussey and Gunn, 1984;Ezhova et al, 1985), and from protoplast cultures (Jacobsen and Kysely, 1984; Puonti-Kaerlas and Eriksson, 1988; Lehminger-Mertens and Jacobsen, 1989). Agrobacterium-mediated transfonnation of various pea explants has also been reported, e.g.…”

mentioning

confidence: 99%

Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.)

et al. 1993

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The pea (Pisum sativum) is an important grain legume crop plant that has gained worldwide economic importance as a source of protein for animal and human nutrition. In addition, it has well-defined genetics, and it has been commonly used as a model plant for research in plant physiology and biochemistry. The productivity and value of peas could be greatly increased by the introduction of stably inherited traits such as pest and disease resistance, herbicide resistance, and improved protein quality. These traits are not available in natural populations of near relatives of cultivated peas, but current advances in plant genetic engineering provide a potentially powerful tool for achieving these goals by another means.The prerequisites for the transfer of foreign genes into any plant species by genetic engineering are an efficient gene delivery system, such as Agrobacterium-mediated DNA transfer, an effective selectable marker for transformed cells, and the ability to regenerate mature, fertile, transgenic plants from transformed tissue in culture.Regeneration via embryogenesis or organogenesis has been described for a variety of pea explants, e.g. from immature leaflets (Mroginski and Kartha, 1981;Rubluo et al., 1984), from cotyledonary node (Jackson and Hobbs, 1990), from hypocotyls (Nielsen et al., 1991), from embryos (Kysely et * Corresponding author; fax 61-6-246-5000. 75 1 Natali and Cavallini, 1987;Tetu et al., 1990), from various organs of seedlings (Malmberg, 1979;Hussey and Gunn, 1984;Ezhova et al., 1985), and from protoplast cultures (Jacobsen and Kysely, 1984; Puonti-Kaerlas and Eriksson, 1988; Lehminger-Mertens and Jacobsen, 1989). Agrobacterium-mediated transfonnation of various pea explants has also been reported, e.g. stem explants (Lulsdorf et al., 199 l), embryonic axis and epicotyl segments (Filippone and Lurquin, 1989;Puonti-Kaerlas et al., 1989), nodus explants (De Kathen and Jacobsen, 1990; Nauerby et al., 1991), and root explants and protoplasts (Schaerer and Pilet, 1991). Tumors were induced in young pea plants by wild-type Agrobacterium . However, no mature transgenic pea plants were regenerated from any of the above transformation systems. The only report to date of stable transformation of peas and the production of mature, flowering, transgenic pea plants is by Puonti-Kaerlas et al. (1990), who achieved regeneration by organogenesis via callus formation using a gene encoding hygromycin phosphotransferase as a selectable marker.In this paper we report the development of a routine, reliable transformation and regeneration system for peas. The procedure has been used to introduce herbicide resistance and the expression of an antibiotic resistance gene into two cultivars of peas using an Agrobacterium tumefaciens-mediated delivery system. Integration of the two traits was stable, and their frequency in the first generation progeny followed the Mendelian pattem. MATERIALS AND METHODS Plant Material and Transformation ProcedurePea (Pisum sativum L.) cv Greenfeast and cv Rondo were grown in t...

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The determination of pea leaves, leaflets, and tendrils

Gould

Cutter

Young

1994

American J of Botany

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Pea leaf determination was examined by culturing excised leaf, leaflet, and tendril primordia ofdifferent ages on a nutrient medium. Pinna primordia were designated as I) determined, if they grew normally in culture; 2) undetermined, if they grew into differentiated structures that were morphologically and anatomically different from either leaflet or tendril; or 3) partially determined, if the two pinnae of an opposite pair developed unequally in isolation, or for leaflet pinnae only, if laminae were initiated but did not develop completely. The compound pea leaf as a whole is determined over four plastochrons of development. Proximal pinnae are determined during the second leaf plastochron, approximately 0.8 plastochron after their initiation. The second most proximal pair of pinnae is determined during the third plastochron, and the terminal portion of the rachis is determined last, during the fourth plastochron. Determination ofleaflet dorsiventrality is gradual, requiring a critical minimum period with the ieafin physiological contact with the shoot system. The rachis primordium, when isolated from the shoot, does not affect determination of its pinnae as leaflets or tendrils. Alila and tendril-less homeotic mutations do not alter the timing of pinna determination.

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Plant production in pea (Pisum sativum L. cvs. Puget and Upton) from long-term callus with superficial meristems

Cited by 51 publications

References 6 publications

Organogenesis and long-term micropropagatlon of Polish pea cultivars

Organogenesis and long-term micropropagatlon of Polish pea cultivars

Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.)

The determination of pea leaves, leaflets, and tendrils

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