Plant Propagation in Vitro: Hormonal Interactions

Werbrouck, Stefaan; Dhuyvetter, Hilde; Pérez, RM; Topoonyanont, Nopmanee; Debergh, Pierre

doi:10.17660/actahortic.2001.560.71

Cited by 1 publication

(2 citation statements)

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“…As reviewed by Fletcher et al [21], certain azole compounds, such as Imazalil interfere with the biosynthesis of gibberellins and influence plant morphogenesis, indicating their possible function as plant bio-regulators. The mode of action of Imazalil is unclear; it only induces shoots in the presence of exogenous cytokinins such as BA, indicating that Imazalil might alter BA metabolism or catabolism [6]. The percentage of vitrified S. raeseri explants was ranged between 0 and 25%.…”

Section: Experiments 1: Effect Of Imazalil On Micropropagation Of S R...mentioning

confidence: 99%

“…A number of interesting side effects of the imidazole fungicide Imazalil can be observed in diverse tissue cultured plant species, i.e. excessive shoot formation in Araceae, inhibition of bushiness in Gerbera, restoration of normal embryo development in Citrus and histogenic instability of Ficus benjamina L. (Moraceae) chimeras [6]. When screening fungicides as potential tissue culture medium additives to control fungal development, it was found that the imidazole fungicide Imazalil exuberantly enhanced the shoot (bud)-inducing effect of BA in Spathiphyllum floribundum.…”

Section: Introductionmentioning

confidence: 99%

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The Stimulating Effects of Imazalil and Carbendazim Fungicides on in Vitro Propagation and ex Situ conservation of the Medicinal Balkan Range-Restricted Sideritis Raeseri Boiss & Heldr. Subsp. Raeseri

Sarropoulou¹,

Maloupa²

2019

JAA

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Sideritis raeseri Boiss & Heldr. subsp. raeseri, known in Greece as Mountain tea of Parnassus or Velouchi is a range restricted medicinal plant of the Balkan peninsula. Conventional propagation methods did not allow the mass production of plant material in a short period of time due to both low seed germination rate and rooting of cuttings. Therefore, the aim of this study was to establish a reliable, reproducible and efficient regeneration protocol for mass and large-scale micropropagation, germplasm and ex situ conservation of S. raeseri Boiss & Heldr. using Imazalil and Carbendazim fungicides. After 9 weeks, 2.5-10 mg/l Imazalil stimulated root length by 1 cm but diminished root number and rooting percentage. Optimum shoot number (5.58), shoot length (24.91 mm), shoot multiplication (100%), root number (20.63) and rooting (66.67%) were recorded with 0.5 mg/l kinetin (KN) + 0.05 mg/l ?-napthaleneacetic acid (NAA) (Imazalil-free). After 4 ½ weeks, 1 mg/l Carbendazim + 0.5 mg/l 6-benzyladenine (BA) yielded 5.77 shoots/explant, 16.1 mm shoot length and 100% shoot multiplication. However, Carbendazim did not result in rooting. The ex vitro survival percentage of rooted shoot-tip explants derived from Imazalil experiment was 95%. Carbendazim proved useful in shoot proliferarion and Imazalil in root elongation of S. raeseri Boiss & Heldr. subsp. raeseri micropropagation system.

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Section: Experiments 1: Effect Of Imazalil On Micropropagation Of S R...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%