Plant Proteinase Inhibitor and Protease Interaction During Insect-Plant Communication

Saikia, Mahaswetta; Kumar, Rohtash

doi:10.1007/978-981-15-2467-7_11

Cited by 1 publication

(1 citation statement)

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“…The elastase reported in the literature have molecular weights in the range of 24-28 kilodalton (kDa), consisting of a single polypeptide chain and most of them have a basic potential isoelectric point (pI) near pH 9 (5,10,18). In 1878, Walchli was the first one to report the elastase activity, where he found that Ox elastase could digest the nuchal ligament elastin, followed him Kuhne who found that crude pancreatic trypsin could dissolve elastin (24). some authors reported many benefits of elastase in food systems such as meat tenderization (2, 12) or medical applications like curing hyperlipidemia and arteriosclerosis (23), preparation of elastin hydrolysate which has beneficial effects on the skin and blood vessels (26) and induced of eht abdominal aortic aneurysm (13).…”

Section: Introductionmentioning

confidence: 99%

Extraction, Purification and Characterization of Elastase From the Digestive Duct of Catfish (Silurus Triostegus)

A. M. Kadhim,

K. A. Shakir

2024

Iraqi J. Agric. Sci.

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This study was aimed to extract, purify and characterize the pancreatic elastase from the Catfish (silurus trioseegus) digestive duct. Different extraction solutions were used to optimize the extraction condition being (Distilled water, 200 mM NaCl, Phosphate buffer (100 mM, pH 8), the latter buffer Tis-HCl (100 mM, pH 8) both containing 0-100 mM NaCl or CaCl2 separately). Tris-HCl buffer containing 100 mM CaCl2 was the best among the rest extraction solutions. The crude extract was precipitated using a 30 % - 55 % of ammonium sulfate saturation ratio. The crude precipitate was dissolved in the same buffer and dialyzed. Then the dialyzed precipitate was concentrated and applied to CM-52 column (3.5 ×10 cm), elastase activity was appeared in two protein peaks. Each peak fraction were pooled individually and loaded on a Sephadex G-75 column (1.6×62 cm). Elastase activity was recovered only from one peak with 29.54 % yield, 530.70 U total activity and 17.89 U/mg specific activity. The optimum temperature for elastase stability and activity was 0-30 C° and 55 C° respectively. while the optimum pH for the enzyme activity and stability were 9 and 4-9 respectively. The molecular weight was 21898 Da as determined by Gel filtration.

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