Plant proteins. IV.—amino‐acids present in alcoholic extracts of grass and legume hays and fenugreek seeds

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Various layers from profiles from upland acid peat area containing both untouched and reclaimed sites were chemically examined for inorganic and organic changes as a result of treatments. Low phosphorus and high aluminium and lead contents were found and a layer of copper accumulation was noted in the undisturbed site. Lanthanum content was also found to be high. The amino‐acid distribution in hydrolysates of soil water, as well as pyrophosphate‐extracted humic acid and the pyrophosphate‐insoluble soil residue containing organic matter, was examined. The surface layers are characterised by the presence of non‐protein amino‐acids of plant and microbiological origin. The basic amino‐acids appeared to be in greater strength in the soil water samples from treated sites than those from untreated sites. The soil residue appears to be a reservoir of amino‐sugar‐containing compounds. The relative distribution of certain “non‐protein” amino‐acids found in small amounts in the humic acids isolated from samples from different depths in the profile, may possibly be explained if the humic acid is considered as a cation‐exchanger being eluted in the hydrogen cycle.

Chemical studies on upland peat in north wales

Coulson¹,

Davies²,

Khan³

1959

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A comparison of the free amino‐acids of two tropical grasses

Hodgson

1964

The individual free amino-acids of the leaf and stem tissues of two related tropical pasture grasses-Bvachiari vuziziensis and Setaria sphacelata-have been quantitatively determined in 17 successive weekly cuttings from the early seedling to the flowering stages, by a paper-chromatographic DNP method. The observed differences in the respective amino-acid patterns of the two species are insufficient to account for the relatively greater accumulation of ammonium-N, during the early growth period, in S. sphacelata which has been previously reported.

Effects of nitrogen and potassium fertilisers on contents of carbohydrates and free amino acids in Italian ryegrass. II. Changes in the composition of the non‐protein nitrogen fraction and the distribution of individual amino acids

Nowakowski

Byers

1972

Italian ryegrass (Lolium multiflorum) S22, grown in a greenhouse in K-deficient soil in pots, was given 40,SO and 160 mg of N/kg of soil (as ammonium nitrate) and 0, 60, 120 and 240 mg of K/kg of soil (as potassium chloride). In grass grown without added K, increasing N fertiliser increased the concentrations of total N, non-protein-N, ammonium-N, nitrate-N, free amino acids, amides and amines. With adequate K fertiliser all these N fractions decreased.The percentage distribution of N in the free amino-acid pool varied with the amount of N and K given. Without K fertiliser, increasing N had no consistent effect on most amino acids and amides, although it decreased the percentages of alanine and ethanolamine. Increasing K had relatively little effect on the percentage distribution in grass given the two smaller amounts of N. However, in the grass given the largest amount of N, it increased the percentage of most amino acids, especially alanine and 4-amino-n-butyric acid, and decreased glutamine and asparagine. p-Alanine was found only in K-deficient plants.The effect of K in relation to N metabolism and the nutritive value of herbage is briefly discussed.