Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydration and assessment of their genetic stability

Scocchi, Adriana; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Olmos, Sofı́a; Mroginski, L. A.

doi:10.1023/b:euph.0000009538.01279.d3

Cited by 39 publications

(21 citation statements)

References 15 publications

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“…Maintenance of the genetic stability of cryopreserved germplasm has been also reported in Melia (Scocchi et al, 2004), Dioscorea (Dixit et al, 2003), and in grape and kiwi (Zhai et al, 2003). Similarly, in our study, plants regenerated from cryopreserved dormant buds using two-step freezing were 100% genetically similar, suggesting that no DNA polymorphisms induced by cryopreservation accumulated (Harding, 2004).…”

Section: Assessment Of Genetic Stability Using Issr Markersmentioning

confidence: 86%

Morphological and Genetic Stability of Dormant Apple Winter Buds After Cryopreservation

Yi¹,

Lee²,

Chung³

et al. 2015

Korean Journal of Plant Resources

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-Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

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Section: Assessment Of Genetic Stability Using Issr Markersmentioning

confidence: 86%

Morphological and Genetic Stability of Dormant Apple Winter Buds After Cryopreservation

Yi¹,

Lee²,

Chung³

et al. 2015

Korean Journal of Plant Resources

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“…formation of an amorphous glassy structure without occurrence of ice crystals which are detrimental to cellular structural integrity. These techniques are more appropriate for complex organs like embryos and shoot apices; they are also less complex and do not require a programmable freezer, hence are suited for use in any laboratory with basic facilities for tissue culture (Charoensub et al 1999;Niino et al 1992a;Scocchi et al 2004). …”

Section: Cryopreservation Proceduresmentioning

confidence: 99%

“…Number of studies indicate that plants recovered from cold storage or slow growth have no genetic alteration (Hao et al 2004;Renau-Morata et al 2006). In this respect, Scocchi et al (2004) tested the genetic fidelity of Chinaberry tree (Melia azedarach L.) plants obtained from cryopreserved apical meristem-tips using isozyme and RAPD markers. The isozyme systems presented identical electrophoretic bands and Rf values.…”

Section: Genetic Stability Of Conserved Collectionsmentioning

confidence: 99%

In Vitro Conservation of Date Palm Germplasm

Bekheet

2011

Date Palm Biotechnology

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Date palm (Phoenix dactylifera L.) germplasm is difficult to conserve and store in the form of offshoots or in field collections. Tissue culture technologies have had a major impact on the ex situ conservation of plant genetic resources. In vitro culture techniques supplement date palm conservation efforts and have been applied to germplasm collection, preservation and rapid clonal multiplication. In vitro storage methods have been developed for preservation of date palm germplasm and can be used efficiently for international exchange of germplasm because of their obvious advantages over in vivo material. Preservation of plant cells, meristems and somatic embryos has become an important tool for long-term storage of germplasm utilizing minimum space and low maintenance. Short-and mid-term storage is achieved by controlling environmental growth conditions and nutrient media composition. Long-term storage has been reported for in vitro cryopreservation of date palm cultures. Encapsulation of plant material in alginate beads has been suggested recently as a possible means of date palm germplasm exchange. Knowledge about germplasm diversity and genetic relationships are highly valuable tools in plant conservation strategies. In this regard, a number of molecular biology methods are currently available for analysis of genetic diversity in date palm genotypes. This chapter discusses the general issues and different aspects of plant biotechnology used for management and conservation of date palm cultivars.

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“…Maintenance of genetic stability of cryopreserved germplasm has been reported in Melia (Scocchi et al, 2004); Dioscorea (Dixit et al, 2003); Grape and Kiwi (Zhai et al, 2003). Similarly in our study, plants regenerated from cryopreserved dormant buds were 100% genetically similar.…”

Section: M1-mentioning

confidence: 99%

Genetic stability of mulberry germplasm after cryopreservation by two-step freezing technique

Choudhary¹,

Chaudhury²,

Malik³

et al. 2013

Afr. J. Biotechnol.

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Genetic stability of Morus alba, Morus indica, Morus laevigata (indigenous collection) andMorus species (exotic collection) have been studied in in vitro regenerated plants of mulberry (fresh, before and after cryopreservation) using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. This study examined the genetic stability of cryopreserved dormant buds of Morus germplasm that were stored in liquid nitrogen using two-step freezing, then rewarmed and regrown. Dormant buds of mulberry collected during winter period were found suitable for the cryopreservation in liquid nitrogen. In the present study, the plants were regenerated directly from dormant buds (before and after cryopreservation) without intermediary callus phase. These regenerants thus bear low risk of genetic instability. Both the single primer amplification reaction (SPAR) markers showed reproducible and well resolved banding patterns in mulberry germplasm, in which RAPD marker generated a total of 201 bands based on 15 primers; however, ISSR markers were given 145 bands using 11 primers. Both markers showed monomorphic banding patterns and did not reveal any polymorphism among the mother plant and in vitro regenerants before and after cryopreservation, suggesting that cryopreservation, using two-step freezing, does not affect genetic stability of mulberry germplasm. The transitory nature of these polymorphisms should be carefully considered when monitoring for genetic stability.

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Plant recovery of cryopreserved apical meristem-tips of Melia azedarach L. using encapsulation/dehydration and assessment of their genetic stability

Cited by 39 publications

References 15 publications

Morphological and Genetic Stability of Dormant Apple Winter Buds After Cryopreservation

Morphological and Genetic Stability of Dormant Apple Winter Buds After Cryopreservation

In Vitro Conservation of Date Palm Germplasm

Genetic stability of mulberry germplasm after cryopreservation by two-step freezing technique

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