Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)

Abstract

-Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.

Comparative untargeted metabolomic analysis of Korean soybean four varieties (Glycine max (L.) Merr.) based on liquid chromatography mass spectrometry

“…조직배양 기술을 필요로 하여 국내에서도 이를 위하여 형질전 환 순응형 품종 선발 연구가 이루어졌으며 [2,3], 나물용인 내병 소립계통의 광안(수원159호)을 중심으로 주로 GM콩 개발 연구 가 수행되었다. 또한 최근 다양한 육종 소재 개발을 위해서 농 업적 가치가 높은 풍원, 태광 등 여러 품종에서 형질전환 방법 이 연구되고 있다 [4][5][6]. 국내에서 광안을 모본으로 하여 linolenic acid 강화콩 [7], β-carotene 강화콩 [8], 가뭄저항성콩 [9], 다수성콩 [10]등 영양성분 강화 및 환경스트레스 저항성, 농업형질 개선 등 여러 종류의 GM콩이 개발되었다.…”