Plant regeneration from alginate-encapsulated shoot tips of Phylianthus amarus schum and thonn, a medicinally important plant species

Singh, Ajay; Sharma, Madhu; Varshney, Rachna; Agarwal, Shradha; Bansal, Kailash C.

doi:10.1079/ivp2005735

Cited by 75 publications

(38 citation statements)

References 17 publications

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“…This difference in response may be due to higher requirement of MS salts and vitamins. Similar observations were also made in banana (Ganapathi et al 2001), Phyllanthus amarus (Singh et al 2006a), guava (Rai et al 2008a).…”

Section: Resultssupporting

confidence: 83%

“…The reason for such differences in response may be due to greater mitotic activity in shoot tips than in lateral buds, which are subjected to apical dominance (Verma et al 2010). Recently, micropropagation through encapsulated shoot tips has also been reported in several economically important plants (Singh et al 2006a;b;Faisal and Anis 2007;Rai et al 2008a;Ray and Bhattacharya 2008;Verma et al 2010). Critical evaluation of concentrations of sodium alginate and calcium chloride which affects the gel matrix and capsule quality is one of the important aspects for the successful propagation of plants through encapsulation technology.…”

Section: Resultsmentioning

confidence: 99%

“…Successful cases of synthetic seed production and plantlet regeneration have been reported for a wide range of plants including cereals, vegetables, fruits, ornamentals, medicinal plants and forest trees (Redenbaugh et al 1991;Castillo et al 1998;Ara et al 2000;Mandal et al 2000;Rai et al 2009). In most cases somatic embryos have been used in the encapsulation process, however, in recent years adequate efforts have also been made to explore the possibility of encapsulating non-embryogenic, in vitro-derived vegetative propagules such as axillary buds, shoot tips or nodal segments for synthetic seed production (Mathur et al 1989;Ganapathi et al 1992;Sharma et al 1994;Ara et al 2000;Mandal et al 2000;Rout et al 2001;Chand and Singh 2004;Naik and Chand 2006;Singh et al 2006aSingh et al , b, 2009Singh et al , 2010Faisal and Anis 2007;Micheli et al 2007;Rai et al 2008a, b;Lata et al 2009;Sundararaj et al 2010;Verma et al 2010). Encapsulation of vegetative propagules could be used for mass propagation of high value crops at a reasonable cost especially when somatic embryos are not available for encapsulation.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Kumar

Manoj

Singh

et al. 2010

Physiol Mol Biol Plants

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Shoot tips excised from in vitro proliferated shoots derived from nodal explants of jojoba [Simmondsia chinensis (Link) Schneider] were encapsulated in calcium alginate beads for germplasm exchange and distribution. A gelling matrix of 3 % sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Best response for shoot sprouting from encapsulated shoot tips was recorded on 0.8 % agarsolidified full-strength MS medium. Rooting was induced upon transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l −1 IBA. About 70 % of encapsulated shoot tips were rooted and converted into plantlets. Plants regenerated from encapsulated shoot tips were acclimatized successfully. The present encapsulation approach could also be applied as an alternative method of propagation of desirable elite genotype of jojoba.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Kumar

Manoj

Singh

et al. 2010

Physiol Mol Biol Plants

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“…Later, Redenbaugh et al (1984) were successful in producing synthetic seeds for alfalfa by encapsulating somatic embryos with alginate hydrogel. Since then several scientists have been working on synthetic seeds incorporating encapsulation technology with different plant species of medicinal plants (Faisal & Anis 2007, Singh et al 2006a, b, 2009 (Rout et al 2000). A method has been developed for rapid multiplication of C. orchioides through direct organogenesis and bulbil formation in-vitro (Suri et al 1999).…”

Section: Biotechnological Tools In Conservationmentioning

confidence: 99%

Ethnomedicinal Plants of India with Special Reference to an Indo-Burma Hotspot Region: An overview

Kumar

Lalramnghinglova

2011

Ethnobot. Res. App.

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“…Therefore, as with synthetic seed, micropropagation is also a modality within tissue culture, but the main differential advantages that are objected when using the synthetic seed technique are associated with the elimination of the final phase of micropropagation (that is, rooting and acclimatization) (SINGH et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

et al. 2017

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-The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of 'Prata-anã' banana's microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl 2 .2H 2 O (100 mM) for complexation and then in KNO 3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 μmol L -1 ) associated with NAA (naphthalene acetic acid) 0.54 μmol L -1 , totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 μmol L -1 in the capsule composition are not indicated for microshoots growth. Index terms: encapsulation, micropropagation, Musa sp., growth regulators. CRESCIMENTO DE MICROBROTOS DE BANANEIRA 'PRATA-ANÃ' CLONE GORUTUBA A PARTIR DE SEMENTES SINTÉTICAS: SUBSTRATOS E CONCENTRAÇÃO DE BAPRESUMO-A bananicultura destaca-se como atividade de grande importância social e econômica no Brasil, o qual ocupa o quinto lugar na produção mundial. A produção de semente sintética vem tornando-se promissora para a micropropagação e conservação in vitro. O objetivo deste trabalho foi analisar a conversão e o crescimento de microbrotos de bananeira 'Prata-anã' clone Gorutuba a partir de semente sintética em meio de cultura MS e vermiculita, testando-se diferentes substratos e concentrações de BAP (6-benzilaminopurina), associado a ANA (ácido naftaleno acético), na constituição de sua cápsula. Os microbrotos foram mergulhados à matriz de alginato de sódio (3%) e gotejados em solução de CaCl2. 2H2O (100 mM) para complexação e depois em solução de KNO3 (100 mM) a descomplexação. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2 x 5 (substrato x concentrações de BAP), contendo diferentes substratos (meio de cultura MS e vermiculita) e concentrações de BAP (2,22; 4,44; 6,66; 8,88 e 13,32 μmol L -1 ), associado a ANA (ácido naftalenoacético) 0,54 μmol L -1 , totalizando 10 tratamentos, com 4 repetições, sendo que cada repetição conteve 5 sementes. As avaliações de conversão, nú...

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Plant regeneration from alginate-encapsulated shoot tips of Phylianthus amarus schum and thonn, a medicinally important plant species

Cited by 75 publications

References 17 publications

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Ethnomedicinal Plants of India with Special Reference to an Indo-Burma Hotspot Region: An overview

Growth of ‘Prata-Ana’ Banana’s Microshoots Clone Gorutuba From Synthetic Seeds:substrates and Bap Concentration

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