Plant regeneration from different explant types of Bituminaria bituminosa and furanocoumarin content along plant regeneration stages

Pazos-Navarro, María; Rı́o, José Antonio del; Ortuño, A.; Romero-Espinar, Pascual; Corréal, Enrique; Dabauza, Mercedes

doi:10.1007/s10725-013-9784-9

Cited by 9 publications

(8 citation statements)

References 23 publications

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“…In the present work psoralen and angelicin were found in in vitro plantlets (25AP, 40AP, 25R, 40R), as well as in vivo plants (WAP and WR), as reported in a previous paper [21]. These results are in agreement with a previous study of in vitro cultures of B. bituminosa [16], where furanocoumarins (FC) were detected in in vitro shoots and ex vitro plants. The same authors reported a very low amount of FC in organogenic 4-month-old calli [16].…”

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confidence: 93%

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In vitro Cultures of Bituminaria bituminosa: Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

D’Angiolillo

Pistellia

Noccioli

et al. 2014

Natural Product Communications

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Bituminaria bituminosa L. is known for producing several compounds with considerable pharmaceutical interest, such as phenylpropanoids, furanocoumarins and pterocarpans. In vitro cultures of seedlings, shoots, and callus have been produced to obtain plant materials useful for the production of these metabolites. The secondary metabolite profile was evaluated by HPLC-DAD. The extracts of all the in vitro material contained the flavonoid daidzein, while plicatin B, erybraedin C and bitucarpin A were found only in the extracts of the in vitro shoots and in wild shoots. The furanocoumarins angelicin and psoralen were found in in vivo and in vitro plants, but in the callus were not detectable. The extracts were also tested for cytotoxic activity in HeLa cell culture; the highest level of cytotoxicity was found in in vitro shoot extracts.

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confidence: 93%

“…These results are in agreement with a previous study of in vitro cultures of B. bituminosa [16], where furanocoumarins (FC) were detected in in vitro shoots and ex vitro plants. The same authors reported a very low amount of FC in organogenic 4-month-old calli [16]. No presence of these compounds was found in our callus cultures (4 weeks-old).…”

supporting

confidence: 93%

In vitro Cultures of Bituminaria bituminosa: Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

D’Angiolillo

Pistellia

Noccioli

et al. 2014

Natural Product Communications

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“…Unlike other pasture legumes, tedera is also known to contain furanocoumarins (FCs) such as psoralen and angelicin in its leaves, stems and fruits (Innocenti et al ., ). Furanocoumarins are phytoalexins synthesized in response to stresses such as drought, fungal infection and insects (Pazos‐Navarro et al ., ). Psoralens are germination inhibitors (Baskin et al ., ; Friedman et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula)

Castello

Croser

Lulsdorf

et al. 2013

Grass and Forage Science

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Tedera (Bituminaria bituminosa vars. albomarginata and crassiuscula) is a perennial pasture species with agronomic characters ideally suited to Mediterranean climates. Tedera seed has a period of after-ripening or primary dormancy typically lasting three months, which delays assessment and breeding of elite hybrid varieties. Temperature, chemical and mechanical methods were investigated in conjunction with in vitro culture to circumvent this dormancy period across a range of parental and hybrid genotypes. Temperature treatment of T5 (Tedera accession 5) and T48 (Tedera accession 48) alone was not sufficient to break dormancy (24.0% and 14.7% germination); however, when combined with soaking in gibberellic acid (GA 3 ) and mechanical scarification resulted in 79.7% and 84.3% germination respectively. In an effort to further improve this result for valuable hybrid genotypes, we combined mechanical scarification with removal of seed coat after imbibition and in vitro culture on B5 medium until radicle emergence. This resulted in breaking dormancy from 96% to 100% of parent seeds and 100% of hybrid seeds. Hardening the germinated F 1 or F 2 seedlings 4 d after first transfer to in vitro culture resulted in 100% survival of plants to soil. This procedure is now used on a routine basis in the Australian tedera breeding programme.

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“…Psoralen production from calli was obtained only for Bituminaria bituminosa and Ruta graveolens, and the content was less than 60 μg g -1 DW [30,32]. C. scorpiodes calli produced 137 μg g -1 DW psoralen in derived-root calli.…”

mentioning

confidence: 94%

Coumarin Compounds in Coronilla scorpioides Callus Cultures

Piovan

Filippini

Innocenti

2014

Natural Product Communications

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Coronilla scorpioides (L.) W.D.J. Koch is known for producing several compounds with pharmaceutical interest, such as the hydroxycoumarins umbelliferone, scopoletin and daphnoretin, the dihydrofuranocoumarin marmesin, and the furocoumarin psoralen. In vitro callus cultures of C. scorpioides were established from hypocotyl, leaf, stem internode and root explants in order to evaluate the possibility of in vitro production of these active secondary metabolites. Calli were obtained with high frequency from all the explant types both in B5 and MS medium. However, after the third subculture, B5 medium, giving the best results, was selected for subsequent transfers. Homogeneous calli were kept either in darkness or in light. Chemical analyses showed that scopoletin and the intermediate products of the biogenetic pathway of psoralen, umbelliferone and marmesin, were always present in the calli and excreted into the media, while daphnoretin was never detected. Light seems to be a prerequisite for psoralen biosynthesis. Root-derived calli produced a significantly higher amount of psoralen (137.5 µg g-1 DW). Principal component analysis showed that umbelliferone, marmesin and psoralen contents are related to variables associated with different explant types.

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Plant regeneration from different explant types of Bituminaria bituminosa and furanocoumarin content along plant regeneration stages

Cited by 9 publications

References 23 publications

In vitro Cultures of Bituminaria bituminosa: Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

In vitro Cultures of Bituminaria bituminosa: Pterocarpan, Furanocoumarin and Isoflavone Production and Cytotoxic Activity Evaluation

Breaking primary dormancy in seeds of the perennial pasture legume tedera (Bituminaria bituminosa C.H. Stirt. vars albomarginata and crassiuscula)

Coumarin Compounds in Coronilla scorpioides Callus Cultures

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