Plant regeneration from in vitro leaf tissues of Viburnum dentatum L

Abstract

Shoots were regenerated from in vitro leaf tissues of two genotypes of Viburnum dentatum, a popular shrub species for landscape use. Adventitious shoots were induced when leaf tissues were cultured on woody plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Effects of cytokinin concentration, indole-3-butyric acid (IBA), and dark treatment on shoot regeneration were investigated. Dark treatment for the first 4 weeks of leaf explants cultured in the regeneration medium significantly increased the frequency of regeneration. The highest frequency of shoot regeneration (70%) for 'Synnesvedt' was obtained when leaf tissues were cultured in the medium with 40 lM BA or 8 lM TDZ with 4 weeks dark treatment. The highest frequency of shoot regeneration (90%) for 'MN34' was found in the 4 lM TDZ medium with 4 weeks dark treatment. Addition of IBA significantly enhanced shoot regeneration. Ethyl methanesulfonate (EMS) treatment inhibited callus proliferation, particularly in the early stage of callus recovery; however, no significant difference in shoot regeneration among different treatments was observed, indicating that the inhibitory effect of EMS was minimal after calluses re-acquired their capacity to grow and regenerate in the regular medium. Regenerated shoots ([1.5 cm) were rooted in the half-strength MS medium containing 5-10 lM IBA or naphthalene acetic acid (NAA). Rooted plants were transferred to the potting medium and grown in the greenhouse.

Hypocotyl derived in vitro regeneration of pumpkin ash (Fraxinus profunda)

“…* Indole-3-butyric acid (IBA). ** Indole-3-acetic acid (IAA) and green ash, as well as other woody species using halfstrength MS basal medium (Dai et al 2011;van Sambeek et al 2001;Zhu and Wei 2010). Exogenous auxin had no significant effect on the number of lateral roots formed compared to the control, except for the treatment of 4.9 lM IBA plus 2.9 lM IAA.…”

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

“…The factors involved for optimum growth of plant includes sterilization, pH and light temperature. As for example, pH is maintained at 5.8 throughout the plant tissue culture before being autoclaved [7]. Then, for growing temperature in the growth chamber is at 25°C [6].…”

Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari

“…The growth curve of Bacupari calli indicated slow growth. According to Dai et al (2011), woody species are challenging because they have long life cycles and little genetic information available. Thus, future studies should be useful to provide information about the slow growth observed.…”