Plant Regeneration From Organ Expiants and Protoplasts of the Medicinal Plant Solatium khasianum C. B. Clarke var. chatterjeeanum Sengupta (Syn. Solatium viarum Dunal)

In vitro organogenesis and plant regeneration from leaves of Solanum candidum Lindl, S. quitoense Lam. (naranjilla) and S. sessiliflorum Dunal

“…Regeneration can also occur with lower frequency in the absence of auxin, as was reported for S. dulcamara [2,14] and S. nigrum [3,14]. The requirement by S. quitoense for GA3 for internodal growth confirms similar GA 3-dependency reported by Kowalczyk et al [9] with S. khasianum and by Roest and Bokelmann [11] with S. tuberosum. The requirement by S. quitoense for GA3 for internodal growth confirms similar GA 3-dependency reported by Kowalczyk et al [9] with S. khasianum and by Roest and Bokelmann [11] with S. tuberosum.…”

“…~3-5 replicates/treatment b3-9 replicates/treatment c3-7 replicates/treatment R = Adventitious roots (+) = Abnormally formed or aborted adventitious shoots + = 50% of explants producing adventitious shoots + + = 50-75% of explants producing adventitious shoots + + + = 76-100% of explants producing adventitious shoots regeneration is dependent on a high cytokinin:low auxin ratio [3,9]. Regeneration can also occur with lower frequency in the absence of auxin, as was reported for S. dulcamara [2,14] and S. nigrum [3,14].…”