Plant Regeneration from Protocorm Like Body (PLB) derived Callus of Dendrobium barbatulum Lindl.

Pyati, A. N.

doi:10.3329/ptcb.v30i2.50694

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Pale green color callus was developed from TDZ hormone with less frequency compared to BAP-induced callus. Similarly, BAP and TDZ proved to be suitable for callus induction and PLB regeneration in D. chrysotoxum (Roy et al 2003) and BAP alone in D. barbatulum (Pyati 2020).…”

Section: Effect Of Cytokinin On Callus Inductionmentioning

confidence: 99%

In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

Kaladharan,

Rengasamy,

Chinnaiyan

et al. 2024

Plant Cell Tiss Organ Cult

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Dendrobium heyneanum Lindl. or Heyne's Dendrobium is an endemic epiphytic orchid of Western Ghats categorized as a threatened taxon. The present investigation was aimed for conservational strategy using in vitro regeneration methods. In this investigation, mature pods of the D. heyneanum were collected from the eld, and were inoculated aseptically on various nutrient media. Half-strength macro-MS media was found to be an e cient asymbiotic seed germination with 86.70% in 12 days old culture. Different stages (I-VI) of morphogenic characters were observed during entire studies; however, 20.84% of seeds produced young seedlings with roots on half-strength macro-MS media. Micropropagation protocol of D. heyneanum was established by using the protocorms (Stage IV) from the asymbiotic germinated seeds. The metamorphosis was observed on half-strength macro-MS forti ed with 0.1 mg/L to 2.0 mg/L of Thidiazuron (TDZ), Benzyl amino purine (BAP) and Kinetin (KN). Higher frequency of protocorms (90.20%) were found in 1.0 mg/L KN with 95.56% of proliferation, shoot number (6.56) and shoot length (1.13 cm). Different frequency of in vitro ower buds was observed in protocorm at 0.5 mg/L (30.2%) and 1.0 mg/L (22.37%); and callus induction at 2.0 mg/L (20.67%) of BAP. Synergistic effect of plantlets were assessed with different concentration of combination of KN (1.0 mg/L) and auxins -α-Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) from 0.1 mg/L to 1.0 mg/L). Combination of KN + IAA (1.0 mg/L) induced elongation, 0.92 cm long pseudobulbs and 0.74 cm rooting. The plantlets were then subsequently acclimatized and hardened on pots containing cocopeat and brick pieces. The survival rate was 52.73%. The present study results nding introduced a protocol for in vitro propagation of Dendrobium heyneanum Lindl. and thus, can be implemented as an ex-situ conservation.

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Section: Effect Of Cytokinin On Callus Inductionmentioning

confidence: 99%

In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

Kaladharan,

Rengasamy,

Chinnaiyan

et al. 2024

Plant Cell Tiss Organ Cult

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“…In another study, culturing the PLB of Aerides crispum Lindl. on ½ MS medium supplemented with BA (1.0 mg L −1 ) + NAA (0.5 mg L −1 ) stimulated the highest percentage of PLB formation (84.3%) (Pyati, 2022). These findings confirmed that orchid species vary in the percentage of PLB formed, as well as, the plant growth regulators (PGRs) needed, and the BA:IBA (mg L -1 ) BA+IBA (mg L -1 ) results were consistent with the results of other studies, where the protocorms of orchid species such as Cypripedium subtropicum S.C.Chen & K.Y.Lang, and Paphiopedilum Alma Gavaert showed low survival rates, which was attributed to vitrification, followed by the browning of tissues, and subsequently, explant death (Hong, Chen, & Chang, 2008;Perner et al, 2022).…”

Section: Plantlet Regenerationmentioning

confidence: 99%

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

Hussien,

Molkanova,

Raeva-Bogoslovskaya

et al. 2024

Ciênc. agrotec.

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In this study, a tissue culture method was developed for the propagation and conservation of Cattleya gaskelliana (N.E.Br.) B.S. Williams. Initially, protocorms with leaf primordium, obtained via asymbiotic seed germination, were used as explants. Half-strength Murashige and Skoog (MS) medium supplemented with 1.5 mg L−1 6-benzyl aminopurine (BA) and 2.0 mg L−1, indole butyric acid (IBA) was found to be optimal for the proliferation of protocorm-like bodies (PLB). Most explants (81%) proliferated, each producing 11.1 ± 1.87 units per explant. The optimum number of PLB differentiated to plantlets on a cultured medium with 0.5 mg L−1 Thidiazuron (TDZ) + 100 mL L−1 coconut water. Firmly rooted plantlets with vigorous growth were regenerated on ½ MS medium + 1.0 mg L−1 Indole- 3-acetic acid (IAA) and 50 g L−1 banana puree. Plants regenerated via in vitro processes were wrapped with sphagnum moss and acclimatized in a substrate containing equal proportions of bark, perlite, and peat with a 100% ex-vitro survival rate after acclimation for 70 days. The protocol developed in this study can be used to obtain several thousand plants within one year, and thus, is an effective method.

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In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

Kaladharan,

Rengasamy,

Chinnaiyan

et al. 2023

Preprint

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Dendrobium heyneanum Lindl. or Heyne’s Dendrobium is an endemic epiphytic orchid of Western Ghats categorized as a threatened taxon. The present investigation was aimed for conservational strategy using in vitro regeneration methods. In this investigation, mature pods of the D. heyneanum were collected from the field, and were inoculated aseptically on various nutrient media. Half-strength macro-MS media was found to be an efficient asymbiotic seed germination with 86.70% in 12 days old culture. Different stages (I-VI) of morphogenic characters were observed during entire studies; however, 20.84% of seeds produced young seedlings with roots on half-strength macro-MS media. Micropropagation protocol of D. heyneanum was established by using the protocorms (Stage IV) from the asymbiotic germinated seeds. The metamorphosis was observed on half-strength macro-MS fortified with 0.1 mg/L to 2.0 mg/L of Thidiazuron (TDZ), Benzyl amino purine (BAP) and Kinetin (KN). Higher frequency of protocorms (90.20%) were found in 1.0 mg/L KN with 95.56% of proliferation, shoot number (6.56) and shoot length (1.13 cm). Different frequency of in vitro flower buds was observed in protocorm at 0.5 mg/L (30.2%) and 1.0 mg/L (22.37%); and callus induction at 2.0 mg/L (20.67%) of BAP. Synergistic effect of plantlets were assessed with different concentration of combination of KN (1.0 mg/L) and auxins - α- Naphthaleneacetic acid (NAA), Indole- 3- acetic acid (IAA), and Indole- 3- butyric acid (IBA) from 0.1 mg/L to 1.0 mg/L). Combination of KN + IAA (1.0 mg/L) induced elongation, 0.92 cm long pseudobulbs and 0.74 cm rooting. The plantlets were then subsequently acclimatized and hardened on pots containing cocopeat and brick pieces. The survival rate was 52.73%. The present study results finding introduced a protocol for in vitro propagation of Dendrobium heyneanum Lindl. and thus, can be implemented as an ex-situ conservation.

show abstract

Plant Regeneration from Protocorm Like Body (PLB) derived Callus of Dendrobium barbatulum Lindl.

Cited by 3 publications

References 10 publications

In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

Plant growth regulators and organic additives on the proliferation of protocorm-like bodies and plantlet regeneration of Cattleya gaskelliana (N.E.Br.) B.S.Williams

In vitro asymbiotic seed germination and micropropagation of Dendrobium heyneanum Lindl. – an endemic orchid of Western Ghats, India

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