Plant regeneration from seedling cotyledons, leaves and petioles of Glycine clandestina

Abstract

Hammatt, N., Davey, M. R. and Nelson, R. S. 1986. Plant regeneration from seedling cotyledons, leaves and petioles of Glycine clandestina. -Physiol. Plant. 68: 125-128.A B5-based culture medium containing 4.4 (iM N'-benzyladenine (BA) and 0.025 \JM indole-3-butyric add {IBA) indueed <^us from seedling cotyledons, leaves and petioles of Glycine ciandestina Wendl. Only hard, green, nodular callus tissues were capable of producing shoot buds and of five accessions examined, only two (G1231 and G1145) were morphogenetically competent. Callus that did not regenerate could often be induced to produce shoot buds after subculture to fresh regeneration medium. Buds developed into shoots following transfer of callus to a medium containing 0.9 (iM BA and 0.025 fiAf IBA. Shoots were rooted in hormone-free, half-strengtb B5 medium supplemented with 0.2% activated charcoal. The application of these results is discussed in relation to somatic hybridisation between the cultivated soybean and wild Glycine species.

Plant regeneration from seedling explants of perennial Glycine species

“…These results, in conjunction with work on G. clandestina [7], show the culture medium to have a major influence on shoot initiation. Although zeatin and the synthetic cytokinins kinetin and 2i-p induced buds in G. canescens Gl171, this response was achieved at a higher frequency using BAP.…”

“…This investigation has established modifications to the procedure described previously for G. clandestina [7] which now permit the regeneration of plants from cultures of other wild Glycine species. These results, in conjunction with work on G. clandestina [7], show the culture medium to have a major influence on shoot initiation.…”

“…2. SC2 medium consisting of basal medium with 1.1 mg 1-1 BAP (6-benzylaminopurine) and 0.005 mgl 1 IBAA (indolebutyric acid) [7]. 3.…”

“…Plants have been produced from explants of G. canescens, G. tomentella [11], and G. clandestina [10], callus derived from embryo cotyledons of G. canescens [5], and seedling-derived callus of G. canescens [20] and G. clandestina [7]. Recently, plants have also been regenerated from protoplasts of G. canescens [16,9] and G. clandestina [9].…”

Studies on soybean tissue culture and transformation

“…but could not root the shoots. Subsequently, whole plants were regenerated from leaves (Hymowitz et al, 1986) and from seedling cotyledons, leaves and petioles (Hammatt et al, 1986) of G. clandestina. cotyledonary nodes of G. soia , and seedling parts of G. canescens.…”