Plant Regeneration from Suspension Cultures of Onion(Allium cepa L.).

Abstract

An efficient method for plant regeneration from suspension cultures of onion was studied. For callus induction, sterilized seeds were inoculated on solid MS medium supplemented with 50 uM 4-FPA, 1 uM 2 iP, 1g/l casein hydrolysate and 0.1 M sucrose. For suspension culture, callus was mechanically disaggregated and transferred into liquid MS medium with 10mM MES added, and placed on a rotary shaker. Suspension cultures were routinely subcultured. After pre-culture in liquid medium which increased sucrose concentration to 0.2 M, two types of cell clumps developed, a deep yellow, small type, and a light yellow, large type. The large type showed higher regenerative ability than the small type . Frequent regeneration of plantlets occurred on solid MS medium supplemented with 1-5 uM 2 iP. Regenerated plantlets developed to whole plants.

A novel autofluorescence-based selection of calli amenable to Agrobacterium-mediated transformation in onion (Allium cepa L.)

“…directly sown on a callus induction medium containing 4-fluorophenoxyacetic acid as auxin (Tanikawa et al 1996). The procedure was successfully applied to 22 cultivars of onion (Tanikawa et al 1998).…”