Plant regeneration from various expiants of cultivated Piper species

Abstract

Morphogenetic potential of root, leaf, node and internode expiants of 3 cultivated Piper species was investigated to develop a reliable plant regeneration protocol. P. longum (pipli) was the most responsive followed by P. betle (betel vine) and P. nigrum (black pepper). In P. longum the highest number of shoot buds was produced on root expiants followed by node, internode and leaf expiants. In P. betle and P. nigrum adventitious shoot buds differentiated only from internodal and nodal ring regions, respectively. Histological examination in P. longum showed that adventitious shoot buds originate directly from the cortical cells of the root and the internode without an intervening callus phase. Benzyladenine was superior to kinetin for shoot induction and its optimum concentrations for P. longum, P. betle and P. nigrum were 1-2, 10 and 10 μM, respectively. Shoot elongation and rooting were achieved in B5 medium containing 0.5 μM benzyladenine and 1 μM indoleacetic acid, respectively. Regenerated plants were established in soil.

Factors affecting in vitro propagation of Momordica balsamina: a medicinal and nutritional climber

“…1c). The stimulating effect of BAP on bud break and multiple shoot formation has been reported earlier by Bhatt et al (1995).…”

In vitro propagation of Homalomena aromatica Schott., an endangered aromatic medicinal herb of Northeast India

“…The promotory effect of BAP on multiple shoot induction has been reported earlier in other medicinally important plant species such as Zingiber officinale (Balachandran et al 1990), Piper spp. (Bhat et al 1995), Houttuynia cordata (Handique and Bora. 1999), and Curcuma longa (Rahman et al 2004).…”

Concentrações de citocinina e carvão ativado na micropropagação de pimenta-do-reino

“…Segmentos nodais funcionaram como os melhores explantes para a regeneração de pimenta-doreino, segundo BHAT et al (1995), que verificaram que cada segmento poderia gerar uma nova planta e, também, brotações. Dessa forma, foi contabilizado o número de segmentos nodais gerados por tratamento.…”

“…Protocolos de regeneração in vitro para a pimenta-do-reino já foram descritos, (PHILIP et al, 1992;BHAT et al, 1995), porém utilizando cultivares indianas. Não são conhecidos trabalhos de multiplicação in vitro envolvendo cultivares brasileiras ou adaptadas ao Brasil.…”