Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 1: The role of antioxidant enzymes

Filipović, Biljana; Simonović, Ana; Trifunović, Milana; Dmitrović, Slavica; Savić﻿﻿﻿, Jelena; Jevremović, Slađana; Subotić, Angelina

doi:10.1007/s11240-015-0740-4

Cited by 29 publications

(36 citation statements)

References 53 publications

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“…Through in vitro culture of selected explants, continuous, reliable source of natural products could be provided (Caruso et al, 2013;Karuppusamy, 2009). Although centaury is characterized with extraordinary manageability in vitro and great regeneration ability (Filipović et al, 2015), to the best of our knowledge, the information on the effects of gene dosage on regeneration capacity is lacking. The aim of this study was to examine the impact of ploidy level on regenerative in vitro potential and biosynthetic capacity of Centaurium erythraea.…”

Section: Introductionmentioning

confidence: 99%

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

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The impact of ploidy level on both the regenerative potential under in vitro conditions and the production of major bioactive specialized metabolites, such as iridoids and xanthones, was examined in Centaurium erythraea Rafn. Shoot regeneration frequency was genotype dependent, but not affected by explant ploidy level. In most cases, the regenerated shoots of autotetraploid (4x) genotypes were more robust than diploid (2x) ones. Regeneration efficiency of root explants declined from the apical to the basal root segment. Shoot and root biomass production of two month-old plants was not significantly different between 2x and 4x genotypes. Both 4x and 2x genotypes were characterized by the predominance of secoiridoid glucoside gentiopicrin in shoots and roots, which is followed by sweroside and swertiamarin. Loganic acid, loganin and secologanin were much less abundant. Methylbellidifolin was the major xanthone in both shoots and roots. Diploid plants showed higher biosynthetic capacity for the production of secoiridoids and xanthones in both shoots and roots. Results highlight a higher potential of diploid C. erythraea genotypes for biotechnology-based sustainable production of secoiridoids in comparison to tetraploid genotypes.

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Section: Introductionmentioning

confidence: 99%

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Filipović¹,

Šiler²,

Nestorović-Živković³

et al. 2019

Lekovite sirovine

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“…However, data on possible high-frequency regeneration and direct organogenesis from the five explants of S. nigrum are scarce. For some of the endangered or high medicinal value species, the clonal propagation is essential (Filipović et al 2015). Shoots, leaves, nodal segments and less frequently, roots are used as explants to promote organogenesis phenomena (Mallón et al 2011;Pourebad et al 2015).…”

Section: Discussionmentioning

confidence: 99%

A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

Zou¹,

Yang²,

Wu³

2017

JSM

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“…Environmental changes such as seasonal variation, light and temperature affect antioxidant enzyme activities in in vitro-propagated plants [32][33][34]. Stress conditions inhibit the plant growth rate and antioxidant enzyme activities.…”

Section: Discussionmentioning

confidence: 99%

A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro- propagated plants of endemic digitalis trojana Ivanina

Çördük¹,

Demirbaş²,

Hacioglu³

2017

ARCH BIOL SCI BELGRA

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The antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro-propagated plants of Digitalis trojana Ivanina (Helen of Troy foxglove), a perennial endemic plant species of Turkey, were compared. The field work was carried out in May and July 2014, and plant samples of D. trojana were collected from Kazdağı (Turkey). Propagation of D. trojana was achieved by culturing leaf explants on MS medium supplemented with 13.3 µM 6-benzylaminopurine (BA) and 0.53 µM α-naphthaleneacetic acid (NAA). The antimicrobial activity, plant lipid peroxidation levels and antioxidant enzyme (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR)) activities were analyzed in 12-and 17-week-old in vitro-grown Digitalis plants collected in May and July from two different localities at 430 and 1173 m above sea level. Although the in vitro-propagated plants had very low antagonistic activities compared to field-grown plants, they exhibited remarkably similar antibacterial activities against Escherichia coli ATCC 11230, Pseudomonas aeruginosa ATCC 27853 and Bacillus subtilis ATCC 6633. There were no important differences between plants collected from the two localities (430 and 1173 m a.s.l.). Biochemical analysis showed that the antioxidant enzyme (SOD, APX, GR) activities of field-grown plants were higher than in vitro-grown plants. Also, the difference in altitude at which the plants were grown was apparently linked to decreases in antioxidant enzyme activities, except for POX in field-grown plants collected in July.

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Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 1: The role of antioxidant enzymes

Cited by 29 publications

References 53 publications

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

Diploid vs. tetraploid Centaurium erythraea Rafn: A comparative study of regenerative in vitro potential and biosynthetic capacity

A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

A comparative study of the antimicrobial properties and antioxidant enzyme activities of field-grown and in vitro- propagated plants of endemic digitalis trojana Ivanina

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