Plant regeneration in pigeonpea [ Cajanus cajan (L.) Millsp.] by organogenesis

Mohan, M.; Krishnamurthy, K. V.

doi:10.1007/s002990050469

Cited by 42 publications

(30 citation statements)

References 11 publications

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“…However, lower concentrations of sucrose (<1.5 %) in the medium caused leaf yellowing due to carbon deficiency. MS medium combined with 1.0 mg L −1 NAA resulted 90 % rooting (Eapen et al 1998), and 1 mg L −1 IBA had 80-85 % rooting (Mohan and Krishnamurthy 1998). Finally the rooted shoots were transferred to the autoclaved soil for hardening and grown till maturity (Fig.…”

Section: Elongated Shoot Rootingmentioning

confidence: 99%

“…In pigeon pea, the regeneration of plants by organogenesis has been reported via pre-existing meristems like apical meristem (Cheema and Bawa 1991), undifferentiated callus (Kumar et al 1983;George and Eapen 1994), differentiated non-meristematic leaf tissue Eapen et al 1998;Geetha et al 1998;Singh et al 2002;Dayal et al 2003;Villiers et al 2008); and seedling tissues such as hypocotyl (Cheema and Bawa 1991;Geetha et al 1998), cotyledon (George and Eapen 1994;Geetha et al 1998;Mohan and Krishnamurthy 1998;Chandra et al 2003), cotyledonary node (Mehta and Mohan Ram 1980;Kumar et al 1983Kumar et al , 1984Naidu et al 1995;Shiva Prakash et al 1994;Geetha et al 1998;Singh et al 2003), epicotyl (Kumar et al 1984;Naidu et al 1995;Geetha et al 1998), and embryonal axes (Sarangi and Gleba 1991;George and Eapen 1994;Naidu et al 1995;Franklin et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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In vitro regeneration of pigeon pea through organogenesis and somatic embryogenesis was demonstrated with pigeon pea cv. JKR105. Embryonic axes explants of pigeon pea showed greater regeneration of shoot buds on 2.5 mg L −1 6-benzylaminopurine (BAP) in the medium, followed by further elongation at lower concentrations. Rooting of shoots was observed on half-strength Murashige and Skoog (MS) medium with 2 % sucrose and 0.5 mg L −1 3-indolebutyric acid (IBA). On the other hand, the regeneration of globular embryos from cotyledon explant was faster and greater with thidiazuron (TDZ) than BAP with sucrose as carbohydrate source. These globular embryos were maturated on MS medium with abscisic acid (ABA) and finally germinated on half-strength MS medium at lower concentrations of BAP. Comparison of regeneration pathways in pigeon pea cv. JKR105 showed that the turnover of successful establishment of plants achieved through organogenesis was more compared to somatic embryogenesis, despite the production of more embryos than shoot buds.

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Section: Elongated Shoot Rootingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

Krishna

Reddy

Ramteke

et al. 2011

Physiol Mol Biol Plants

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“…Shoot bud induction from cotyledonary callus of pigeon pea on Blaydes medium with 2.25 mgL -1 BAP has been reported (Kumar et al, 1983). Similarly, 65% shoot buds from cotyledonary node explants of cultivar T-15-15 and SPMA-4 has been reported (Shiva et al, 1994;Mohan and Krishnamurthy, 1998). However, the maximum number of shoot buds regenerated per explants is not clear.…”

Section: Resultsmentioning

confidence: 93%

“…In case of cultivars IPA-337 and IPA-61 lower concentration of kinetin that is, 1.0 and 1.5 mgL -1 was found to be effective for shoot bud induction. Regeneration using distal cotyledonary segment via organogenesis for diverse cultivar BDN-1, BDN-2, Gaut-82-90, ICP 7182, ICPL 87, ICPL 87119, T-15-15, TV-1 has been reported in the presence of kinetin, BAP and /or adenine sulphate (AdS) (Mohan and Krishnamurthy, 1998). The cultivar T-15-15 showed a maximum of 33 shoots in the presence of BAP + KIN + AdS.…”

Section: Resultsmentioning

confidence: 99%

“…In vitro regeneration by organogenesis using diverse explants like leaf discs (Kumar et al, 1983;Eapen and George, 1993;George and Eapen, 1994;Eapen et al, 1998;Geetha et al, 1998;Dayal et al, 2003), cotyledons (Kumar et al, 1983;Mehta and Mohan, 1980;Kumar et al, 1984;Sarangi and Gleba, 1991;Naidu et al, 1995;Srinivasan et al, 2004;Chandra et al, 2003), cotyledonary nodes (Geetha et al, 1998;Shiva et al, 1994) and embryonal axes (George and Eapen, 1994;Sarangi and Gleba, 1991;Naidu et al, 1995;Franklin et al, 2000;Mohan and Krishnamurthy, 1998) has been reported. More than 50 diverse genotypes have been attempted for regeneration via organogenesis to develop suitable regeneration protocol amenable to transformation with desired genes (Krishna et al, 2010).…”

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confidence: 99%

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In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

Kashyap¹,

Sarangi²,

Yadav³

et al. 2014

Afr. J. Biotechnol.

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Pigeonpea

Sharma

Bhatnagar‐Mathur

Saxena

et al. 2008

Compendium of Transgenic Crop Plants

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Pigeonpea is one of the major grain legumes grown in the tropics and subtropics of the world that forms a significant component of the diet due to its high protein content. Most of the differences in potential and realized yields in pigeonpea have been attributed to several biotic and abiotic constraints; besides the low productivity potential of marginal lands, where this crop is commonly grown. Of the various constraints limiting pigeonpea production, insect pests cause substantial damages. Conventional breeding efforts in pigeonpea crop improvement have been successful in producing improved seed quality and reduction of crop maturity duration. Nevertheless, genetic improvement of pigeonpea has been restricted due to the nonavailability of better genetic resources and strong sexual barriers between the cultivated and wild species. The recent developments in plant genetic engineering have provided immense potential in overcoming some of these constraints, thereby, offering opportunities for its successful integration with conventional crop improvement strategies. This chapter describes the pigeonpea crop, various constraints to its productivity, recent developments in its breeding, and emerging transgenic innovations that could play a significant role in the improvement of pigeonpea crop. We also highlight the current status of pigeonpea transgenics and related biosafety and IPR issues for the successful application of this technology in the near future.

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Plant regeneration in pigeonpea [ Cajanus cajan (L.) Millsp.] by organogenesis

Cited by 42 publications

References 11 publications

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro regeneration through organogenesis and somatic embryogenesis in pigeon pea [Cajanus cajan (L.) Millsp.] cv. JKR105

In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars

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