Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Chen, Chunli; Hu, Yuxin; Ikeuchi, Momoko; Jiao, Yuling; Prasad, Kalika; Su, Ying Hua; Xiao, Jun; Xu, Lin; Yang, Weibing; Zhao, Zhong; Zhou, Wenkun; Zhou, Yun; Gao, Jian; Wang, Jia-Wei

doi:10.1007/s11427-024-2581-2

Sci. China Life Sci.

2024

DOI: 10.1007/s11427-024-2581-2

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Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Chunli Chen,

Yuxin Hu,

Momoko Ikeuchi

et al.

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Cited by 10 publications

References 366 publications

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Hormone functions in adventitious root formation during cutting propagation of woody plants

Liu,

Li,

et al. 2024

J Plant Res

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Hormone functions in adventitious root formation during cutting propagation of woody plants

Liu,

Li,

et al. 2024

J Plant Res

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Identification of quantitative trait loci for in vitro plant regeneration from leaf microexplants in cucumber (Cucumis sativus L.)

Słomnicka,

Cieplak,

Antosiewicz

et al. 2024

J Appl Genetics

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Plant regeneration in tissue cultures is crucial for the application of biotechnological methods to plant breeding. However, the genetic basis of in vitro plant regeneration is not fully understood. For cucumber, regeneration protocols from different types of explants have been reported, but thus far, the molecular basis of regeneration from cotyledon explants has only been studied. The aim of this work was to identify quantitative trait loci (QTLs) for in vitro plant regeneration from cucumber leaf microexplants. Plant regeneration was evaluated using a population of recombinant inbred lines (RILs) developed from a cross between line B10, characterized by high regeneration efficiency, and the low regeneration efficiency line Gy14. All RILs were scored for frequency of callus formation, organogenesis, and shoot regeneration. RILs with regeneration efficiencies higher than that of line B10 have been observed. QTLs for the frequency of organogenesis and shoot regeneration were identified. All the QTLs were mapped on cucumber chromosome 6, explaining 11.9 to 20% of the phenotypic variance. The major-effect QTL for organogenesis or6.1 was located on the upper arm of chromosome 6. The QTLs for shoot regeneration frequency, sr6.1A and sr6.1B, were located on the lower arm of chromosome 6. Analysis of the genomic region corresponding to these QTLs combined with gene expression profiling revealed that CsARF6 and CsWOX9 are gene candidates underlying these QTLs. This study is a step toward identifying the genes controlling the ability of cucumber plant regeneration from leaf explants.

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Autophagy is essential for somatic embryogenesis in citrus through regulating amyloplast degradation and lipid homeostasis

Gao,

Zhao,

et al. 2024

New Phytologist

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Summary Autophagy is a conserved degradation pathway that regulates the clearance of paternal substrate at the early embryogenesis stage of animals. However, its mode of action is likely different in plants, which can regenerate through apomixis without fertilisation. Somatic embryogenesis (SE) is a unique plant process widely used for plant propagation and germplasm utilisation. Here, we studied citrus as an example and found a higher autophagic activity after SE initiation. Interestingly, amyloplasts were frequently found inside autophagosomes, whereas the inhibition of autophagy blocks amyloplasts/starch degradation and hinders somatic embryo formation. Furthermore, the consumption of storage lipids was faster in autophagy mutants, suggesting lipid metabolism is activated when starch utilisation is blocked. Exogenous application of autophagy‐inducing chemicals (e.g. spermidine) significantly promoted the formation of autophagosomes and increased SE efficiency, indicating a positive correlation between autophagy, energy metabolism, and somatic embryo formation in citrus. Taken together, our study unveils a pathway for the degradation of plant‐specific organelles and provides an effective approach for plant propagation.

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Plant regeneration in the new era: from molecular mechanisms to biotechnology applications

Cited by 10 publications

References 366 publications

Hormone functions in adventitious root formation during cutting propagation of woody plants

Hormone functions in adventitious root formation during cutting propagation of woody plants

Identification of quantitative trait loci for in vitro plant regeneration from leaf microexplants in cucumber (Cucumis sativus L.)

Autophagy is essential for somatic embryogenesis in citrus through regulating amyloplast degradation and lipid homeostasis

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