Plant Regeneration of Peppermint, Mentha piperita, from the Hairy Roots Generated from Microsegment Infected with Agrobacterium rhizogenes

Inoue, Fumiyuki; Sugiura, Hiroyuki; Tabuchi, Akira; Karasawa, Den'ei; Minami, Mineo

doi:10.5511/plantbiotechnology.20.169

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…The performed cultivation lasted 6 weeks in total: 2 weeks from sowing to seedling and 4 weeks from seedling to harvest. The day/night ratio was set to 2:1, i.e., 16 h of light (day) and 8 h of darkness (night) [29]. During the cultivation period, the average environmental parameters were as follows: temperature, 25 • C [30], humidity, 50%, carbon dioxide content, 420 ppm.…”

Section: Methodsmentioning

confidence: 99%

Examination of Spectral Properties of Medicinal Plant Leaves Grown in Different Lighting Conditions Based on Mint Cultivation

Feldzensztajn

Wierzba

Mazikowski

2021

Sensors

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Cultivation in controlled environmental conditions can provide good quality medicinal herbs with consistent properties. A sensing system that can determine the contents of medicinal substances in plants using spectral characteristics of leaves would be a valuable tool. Viability of such sensing approach for mint had to be confirmed experimentally, as no data correlating contents of medicinal substances with spectral characteristics of leaves are available, to the best of authors’ knowledge. In the first stage, presented in this paper, the influence of lighting on mint (Mentha rotundifolia) grown on a small hydroponic plantation was studied. Spectral characteristics of leaves were recorded by a spectrophotometer and colorimetric analysis was used to investigate the relationship between these characteristics and the spectrum of lighting. Dry mass yield was measured to test its dependence on the lighting. Dependence of chromaticity of leaves on the spectrum of light used in the cultivation was confirmed. Averaged spectra of leaves are distinguishable using a spectrophotometer and—in most cases—by a human observer. A partial correlation is observed between dry mass yield and the spectrum of lighting. Obtained results justify further research into the correlation between lighting and the contents of biological substances in medicinal plants using spectral characteristics of leaves.

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Section: Methodsmentioning

confidence: 99%

Examination of Spectral Properties of Medicinal Plant Leaves Grown in Different Lighting Conditions Based on Mint Cultivation

Feldzensztajn

Wierzba

Mazikowski

2021

Sensors

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“…[81][82][83][84][85][86][87][88][89] Few years ago it was first reported the infection with A rhizogenes of M. piperita and the regeneration of HR and shoots through the transgenic calli. 90 The first HR obtained from in vitro internodes shoots became visible one or two weeks after inoculation. The frequency of HR induction obtained by direct Agrobacterium infection varied according to the plant organ used: the direct infection of leaves induced more roots compared to those obtained from stem.…”

Section: Mint (Mentha Spp)mentioning

confidence: 99%

“…An 1.6 kb fragment, which included the region from the rolA to rolB gene, was detected in all regenerated plants. 90 Other mint species as Mentha pulegium clonal line MPH-4, a species containing high levels of endogenous phenolics was also inoculated with A. rhizogenes. 91 The inoculated explants exhibited higher levels of total plant phenolic components and guaiacol peroxidase activity.…”

Section: Mint (Mentha Spp)mentioning

confidence: 99%

Hairy Root Cultures for Secondary Metabolites Production

Pistelli¹,

Giovannini²,

Ruffoni³

et al. 2010

Advances in Experimental Medicine and Biology

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Hairy roots (HRs) are differentiated cultures of transformed roots generated by the\ud \ud infection of wounded higher plants with Agrobacterium rhizogenes. This pathogen\ud \ud causes the HR disease leading to the neoplastic growth of roots that are characterized\ud \ud by high growth rate in hormone free media and genetic stability. HRs produce the same\ud \ud phytochemicals pattern of the corresponding wild type organ. High stability and productivity\ud \ud features allow the exploitation of HRs as valuable biotechnological tool for the production of\ud \ud plant secondary metabolites. In addition, several elicitation methods can be used to further\ud \ud enhance their accumulation in both small and large scale production. However, in the latter\ud \ud case, cultivation in bioreactors should be still optimized. HRs can be also utilised as biological\ud \ud farm for the production of recombinant proteins, hence holding additional potential for industrial\ud \ud use. HR technology has been strongly improved by increased knowledge of molecular\ud \ud mechanisms underlying their development. The present review summarizes updated aspects of\ud \ud the hairy root induction, genetics and metabolite productio

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“…It is the natural hybrid (2n = 6x = 72) between M. spicata L. (spearmint, 2n = 48) and M. aquatica L. (water mint, 2n = 96) (Kokkini 1991;Banthorpe 1996;Pushpangadan and Tewari, 2006). They are of major medicinal and aromatic crops and cultivated widely for their essential oil (Inoue et al, 2003) Menthol, the main component of Mentha oil, is found in the highest concentrations in mature leaves (Fahlen et al, 1997;Rohloff 1999). Mentha genus plants produce a natural chemical product group known as monoterpenes and are the only source to produce menthol throughout the world (Chakraborty and Chattopadhyay, 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of Jasmine oil and Methyl Jasmonate on Gene Expression and Menthol Production in Mentha

El-Leel

Mohamed

El-Aziz

2021

Scientific Journal of Agricultural Sciences

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Mentha is the major source of menthol used for many important commercial and pharmaceutical purposes. For enhanced menthol production, this study aimed to assess the influence of the foliar application of Jasmine oil and Methyl Jasmonate by 0.05 and 0.1% on gene expression, essential oil, and its component especially Menthol in Mentha piperita L. Exogenously foliar application of Methyl Jasmonate (MeJA) and Jasmine oil (JO) treatments induced alteration of gene expression patterns which was detected by a cDNA -SCoT technique with high informative and discrimination capability. Where it targeted 33 clear transcript-derived amplicons, 21 out of them were new transcripts. Differential expression analysis of these patterns illustrated that the higher influence was by J asmine oil with concentration 0.1% at 48 h followed by MeJA-0.1% at 24 h. On the other hand, mfs -transcripts were differentially regulated by MeJA and JO treatments. In general, the results revealed high level of volatile oil, high percentages of menthol (1.52 and 1.47-fold) with the low percentages of the menthone (0.69 and 0.77-fold), lowest level of upregulated menthofuran gene expression (-0.88 and -0.72 Log 2 FC). Besides, good vegetative growth relative to the control was found with exogenously foliar application of MeJA by 0.05% after 48 h and JO by 0.1% after 24 h, which increases the pharmacological quality of Mentha plant. Subsequently, indicating the possibility of using JO (Low-cost) after further study as a stimulant for the activity of menthol production with high quality as a cheaper alternative to exogenously foliar application of MeJA.

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Plant Regeneration of Peppermint, Mentha piperita, from the Hairy Roots Generated from Microsegment Infected with Agrobacterium rhizogenes

Cited by 7 publications

References 15 publications

Examination of Spectral Properties of Medicinal Plant Leaves Grown in Different Lighting Conditions Based on Mint Cultivation

Examination of Spectral Properties of Medicinal Plant Leaves Grown in Different Lighting Conditions Based on Mint Cultivation

Hairy Root Cultures for Secondary Metabolites Production

Influence of Jasmine oil and Methyl Jasmonate on Gene Expression and Menthol Production in Mentha

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