Plant regeneration through direct and indirect organogenesis, phyto-molecular profiles, antioxidant properties and swertiamarin production in elicitated cell suspension cultures of Swertia minor (Griseb.) Knobl

Kshirsagar, Parthraj R.; Mohite, Ashwini; Suryawanshi, Sachin R.; Chavan, Jaykumar J.; Gaikwad, Nikhil B.; Bapat, Vishwas A.

doi:10.1007/s11240-020-01962-8

Cited by 20 publications

(4 citation statements)

References 30 publications

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“…Half strength MS medium supplemented with 3.0 mg/l IBA possessed the highest frequency of root induction (95%) and number of roots per explant (10.2) as well as mean root length (2.6 cm). IBA induced root induction has been reported in other systems like Plumbago zeylanica (Sharma and Agarwal 2018), Pterocarpus marsupium (Ahmad et al 2021) and Swertia minor (Kshirsagar et al 2021) In conclusion, a robust shoot regeneration system through ower bud derived callus organogenesis has been standardized for G. febrifugum. This protocol could be utilized for the mass multiplication of this plant for commercial purpose.…”

Section: Rooting Of Shootsmentioning

confidence: 56%

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

Silpa

Thomas

2021

Plant Cell Tiss Organ Cult

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Gymnostachyum febrifugum Benth. is a small, scapigerous, rare and endemic medicinal herb indigenous to India belonging to the family Acanthaceae. This study reports an e cient protocol for high-frequency ower bud derived callus induction and shoot organogenesis in G. febrifugum. Flower buds at 7d before anthesis (dBA) were excised from the in orescence and cultured on MS medium supplemented with various concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D; 0.5-2.0 mg/l) for callus induction. The optimum callus induction (78%) was obtained on MS medium supplemented with 1.5 mg/l 2, 4-D. The calli when subcultured on MS medium supplemented with different concentrations of thidiazuron (TDZ; 0.5-2.5 mg/l) or 6-benzylaminopurine BAP (0.5-2.5 mg/l) alone or in combination with 1naphthaleneacetic acid (NAA; 0.2-0.7 mg/l) induced shoots. The highest frequency (94%) and number of shoots (44.6 shoots/unit callus) were obtained on MS medium supplemented with 2.0 mg/l TDZ and 0.5 mg/l NAA. The optimum rooting frequency (95%) and number of roots (10.2) were observed on ½ MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). The rooted plantlets were acclimatized and transferred to soil with 94% success.

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Section: Rooting Of Shootsmentioning

confidence: 56%

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

Silpa

Thomas

2021

Plant Cell Tiss Organ Cult

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“…Production of genetic clones of the true-too-type plants is the main objective of an in vitro regeneration program. However, obtained plants from an indirect organogenesis pathway usually show slight divergence in genetic composition (Kshirsagar et al 2021). Therefore, assessing the genetic delity of regenerated plants is an essential step in establishing an e cient protocol for the in vitro production of SMs of medicinal plants.…”

Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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Bioactive compounds of medicinal plants have a wide range of applications in pharmaceutical, food and other industries. In vitro culture systems have great potential for sustainable production of bioactive compounds of medicinal plants. In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration e ciency, antioxidants activity and phytochemical pro le of in vitro shoot cultures of ajowan. Different concentrations of salicylic acid (SA) (0, 10, 20, 40, 80 µM) and polyethylene glycol (PEG 6000) (0, 1, 2, 5%) were added to the shoot regeneration Murashige and Skoog medium containing Kin (1.5 mg/L) and NAA (0.25 mg/L) plant growth regulators. Salicylic acid reduced the adverse effect of PEG treatment on number of regenerated shoots and in vitro rooting. The activities of catalase, superoxide dismutase, and peroxidase enzymatic antioxidants were signi cantly increased in SA + PEG treated plants. The gas chromatography-mass spectrometry (GC-MS)-pro ling revealed quantitative and qualitative phytochemical differences between control and SA + PEG treated plants. The greatest means of p-cymene and thymol bioactive compounds were obtained from in vitro shoots treated with 5% PEG + 40 µM SA. The inter-simple sequence repeats (ISSR) markers proved the genetic stability of in vitro regenerated plants. The presented protocol is useful for large-scale sustainable production of secondary metabolites (SMs) of medicinal plants. The same strategy (stress tolerance-inducing elicitor + stress-inducing elicitor) is applicable to increase valuable SMs in other production systems such as hydroponic, greenhouse and eld conditions.

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“…The usage of biotechnology especially plant tissue culture technique for the expansive propagation and preservation of numerous plant groups have been well documented for wild ornamental plants (Raut et al 2006;Chavan et al 2018;Kshirsagar et al 2021). Of the only studies that have been published on micropropagation protocols for B. frerei, development of optimum media composition to initiate shoots and roots (Desai et al 2003).…”

Section: In Vitro Propagationmentioning

confidence: 99%

Morphological characterization, propagation strategy and identification of Boucerosia frerei accessions for their hanging basket exploitation- an attractive succulent with star-shaped flowers from the Northern Western Ghats of India

Bodhe

Patil

Bhosale

et al. 2022

Preprint

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Boucerosia frerei (G. D. Rowley) Mieve & Liede (formerly Frerea indica; family-Apocynaceae) is one of the little known, endemic and critically endangered succulent herbs with pretty flowers distributed sparsely in the Northern Western Ghats of India. Naturally, the plant shows phenotypic variation in numerous qualitative and quantitative characters especially stem, leaves and flowers. Consequently, this is challenging and stimulating taxa that merit considerable further attention of taxonomists, horticulturalist, gardeners, plant enthusiast etc. The present investigation describes the first report of collection, phenotypic characterization, commercially practicable propagation strategy development and identification of the accession for their usage in hanging baskets for B. frerei. The results highlighted some of the important and interesting features of this plant. A total of 32 morphological traits (9 qualitative and 23 quantitative) were noted for five accessions collected from Kate’s Point, Mahabaleshwar (KP), Meruling, Satara (MS), Sajjangad, Satara (SS), Purandar, Pune (PP) and YCIS, Satara (YCIS) and evaluated using ANOVA, PCA and cluster analysis. Ten morphological traits viz. plant height, stem color, number of nodes, number of roots, number of leaves, number of flowers, size, shape, architecture and seed weight contributed to the variation between the accessions. For succulents to be used for hanging baskets, plant height, number of leaves and floral and floral attributes (number, color, size, shape and architecture) are among the most important selection traits. Morphological characterization identified the Meruling, Satara (MS) as the potential accession for hanging baskets. A combination of soil, coconut coir and vermiculite (3:1:1) escalated the phenotypic traits in the aerial parts in hanging basket cultivation. Among the propagation techniques employed, vegetative propagation served superior over tissue-culture technique for large-scale propagation for its conservation and availing the stock material to floriculture industry.

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Plant regeneration through direct and indirect organogenesis, phyto-molecular profiles, antioxidant properties and swertiamarin production in elicitated cell suspension cultures of Swertia minor (Griseb.) Knobl

Cited by 20 publications

References 30 publications

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

High-frequency shoot regeneration from flower bud derived callus of Gymnostachyum febrifugum Benth., an endemic medicinal plant to the Western Ghats

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Morphological characterization, propagation strategy and identification of Boucerosia frerei accessions for their hanging basket exploitation- an attractive succulent with star-shaped flowers from the Northern Western Ghats of India

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