Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts

Dhir, Sarwan K.; Oglesby, Jolethia; Bhagsari, A. S.

doi:10.1007/s002990050462

Cited by 49 publications

(24 citation statements)

References 7 publications

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“…Sin embargo se ha comprobado en varias especies, y en coníferas en particular que, fundamentalmente sobre la inducción de embriogénesis somática, existe una influencia importante del componente aditivo de la varianza genética (Park et al, 1993;Park et al, 1994), lo que permitiría efectuar una mejora genética sobre este carácter. digestión enzimática de las paredes celulares, con base en la tecnología desarrollada para la obtención de protoplastos (Tibok et al, 1995;Dhir et al, 1998). Posteriormente, para lograr la sincronía, la técnica exige el fraccionamiento y subcultivo de las estructuras iniciales.…”

Section: Las Fases De La Regeneración Por Embriogénesis Somática Induunclassified

Somatic embryogenesis as a main tool in forest biotechnology

Celestino¹,

Hernández²,

Carneros³

et al. 2005

For. syst.

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ResumenPara dar sentido agronómico a diferentes biotecnologías de aplicación, esto es, para poder transferir las posibilidades que ofrecen a la actividad operativa, es necesario disponer de una técnica fiable de regeneración de plantas. El cultivo de tejidos de genotipos valiosos, de meristemos libres de virus, de células transformadas, etc., no sirve de nada desde un punto de vista aplicado si de ellos no es posible conseguir la multiplicación en masa de dichos genotipos selectos, obtener plantas saneadas o lograr plantas transgénicas, en condiciones económicamente viables. La embriogénesis somática está ampliamente considerada como la mejor vía de regeneración que utiliza las técnicas de cultivo de tejidos vegetales en biotecnología forestal. Los avances actuales en biotecnología-genómica están teniendo un gran impacto en el uso de material selecto en muchos programas de mejora. La embriogénesis somática está contribuyendo a la producción de plantas transgénicas y a la consecución de la silvicultura clonal de alta productividad, con calidad de madera mejorada y menor impacto de enfermedades, en plantaciones forestales. Este artículo revisa la situación actual de la embriogénesis somática aplicada a especies forestales.Palabras clave: Regeneración de plantas, cultivo in vitro de tejidos vegetales. Summary Somatic embryogenesis as a main tool in forest biotechnologyTo give agronomic value, that is, to be able to transfer the benefits of different biotechnologies of application to operational purposes, a reliable plant regeneration technique is needed. Tissue culture of valuable genotypes, of virus-free meristems, of transformed cells, etc., is not worth if we are not able to mass propagating clonal plants, to obtain healthy plants or to achieve transgenic plants. Somatic embryogenesis is widely assumed to be the best tissue culture-based way of regenerating plants in forest biotechnology. Recent advances in biotechnology-genomics are having an important effect on the deployment of selected genetic plant materials in many breeding programs. Somatic embryogenesis is contributing to the release of transformed plants and to the implementation of clonal forestry with improved productivity, enhanced wood quality and reduced diseases in forest plantations. This article review the current status of somatic embryogenesis applied to forest species.

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Section: Las Fases De La Regeneración Por Embriogénesis Somática Induunclassified

Somatic embryogenesis as a main tool in forest biotechnology

Celestino¹,

Hernández²,

Carneros³

et al. 2005

For. syst.

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“…To compare the effects of mannose on growth of sweetpotato callus with that of negative selection system used in several sweetpotato transformation studies (Dhir et al, 1998;Gama et al, 1996;Morán et al, 1998;Newell et al, 1995;Otani et al, 1993;Otani et al, 1998;Prakash and Varadarajan, 1992;Santa-Maria et al, 2011;Song et al, 2004) and determine the effectiveness and optimum concentration for use in the selection of transformed cells, effects of kanamycin and hygromycin on sweetpotato explants were evaluated. Sweetpotato stem explants were transferred onto supplemented with CIM 30 g/l sucrose.…”

Section: Sensitivity Of To Antibiotics Sweetpotato Callusmentioning

confidence: 99%

Evaluation of the Phosphomannose Isomerase-Based Selection System for Genetic Transformation of Sweetpotato

et al. 2015

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Article InformationIn response to increased public concern on antibiotic or herbicide resistance genes usage in genetically modified plants, mannose was evaluated as selectable agent for the genetic transformation of sweetpotato. Nontransformed sweetpotato stem explants of cv. KSP36 were cultured on media containing various combinations and concentrations sucrose and mannose ranging from 0 to 30 g/l. Likewise, efficacy of hygromycin and kanamycin as selection agents for transformation of sweetpotato was evaluated on media containing varying concentrations of antibiotics ranging 0 to 100 mg/l. Selection agent effectiveness was determined by detecting the minimal concentration of the selection agent required to fully inhibit sweetpotato calli growth. Hygromycin was the most effective selection agent as it inhibited cell growth at concentrations above 20 mg/l. Kanamycin was moderately effective as it inhibited cell growth at 60 mg/l. Sweetpotato calli were able to grow and even produce embryos even when mannose was the only source of carbohydrates.

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“…However, the lack of an efficient regeneration system is the major limiting factor which prevents the development of gene transfer technologies for commercially important cultivars of sweetpotato. There have been a number of proposals for the in vitro propagation of sweetpotato, including via adventitious shoot induction Dessai et al, 1995;Sihachakr et al, 1997;Santa-Maria et al, 2009) and somatic embryogenesis (Liu and Cantliffe, 1984;Chée et al, 1990;Desamero et al, 1994;Bieniek et al, 1995;Zheng et al, 1996;Dhir et al, 1998;He et al, 2009). Genetic transformation of sweetpotato, using various explants, has been reported, but with low efficiency (Newell et al, 1995;Gama et al, 1996;Morán et al, 1998;Okada et al, 2001;Berberich et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…An improvement in the quality of sweetpotato is vital since abiotic stresses, fungal and viral infections, as well as insect pests, adversely affect its productivity. Genetic improvement of sweetpotato via conventional breeding is limited due to male sterility, incompatibility and the hexaploid nature of the plant (Dhir et al, 1998). A more recent technique -genetic engineering -is the only alternative method to improve the productivity of sweetpotato.…”

Section: Introductionmentioning

confidence: 99%

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

Kumar¹,

Ku²,

Agrawal³

et al. 2013

bta

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In this report, a novel, reproducible and efficient protocol for protuberance-mediated adventitious shoot regeneration has been established for sweetpotato [Ipomoea batatas (L.) Lam.]. The leaf explants were cultured on a modified Murashige and Skoog (MS) medium containing various concentrations (0.5, 1.0, 2.0, 5.0 or 10 μM) of Thidiazuron (TDZ). The adventitious shoots were developed from the base of leaf explants on an MS medium supplemented with 2.0, 5.0 or 10.0 μM TDZ. The responsive explants were transferred to a freshly modified MS medium containing TDZ. Green-colored, oval, or cone-shaped shoot-forming protuberances were developed from explants. It appeared that the frequency of protuberance development depended on the concentration of TDZ. However, this did not further improve, when the concentration of TDZ increased from 5.0 μM to 10.0 μM. Adventitious shoot buds were developed from protuberances, and the histology of protuberances revealed a direct origin of shoot buds. In vitro raised shoots were rooted either on a modified MS medium alone or on a modified MS medium containing indole-3-butyric acid (IBA). The regenerated plants were acclimatized and successfully established in a greenhouse.

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Plant regeneration via somatic embryogenesis, and transient gene expression in sweet potato protoplasts

Cited by 49 publications

References 7 publications

Somatic embryogenesis as a main tool in forest biotechnology

Somatic embryogenesis as a main tool in forest biotechnology

Evaluation of the Phosphomannose Isomerase-Based Selection System for Genetic Transformation of Sweetpotato

Protuberance-mediated high frequency of adventitious shoot regeneration from sweetpotato leaf explants [ Ipomoea batatas (L.) Lam.]

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