The Apennine mountain areas suffer progressive abandonment and marginality, although being characterized by an extraordinary richness in natural and cultural resources, and landscapes of great beauty. Therefore, their natural heritage, and especially their geoheritage, tranformed into geotourism initiatives, can represent an essential resource to support local economy andsustainable development. The present study illustrates the case of Matese Mountains (Southern Apennines), particularly rich in protected areas, including the Matese National Park currently taking off, which is characterized by a rich geoheritage, based on 59 geosites. Among these geosites, examining the specially built geosite GIS database, 16 geosites were selected to construct a geoitinerary crossing the Matese Mountains. The geoitinerary was delineated to optimally represent the major geomorphological and geological (especially geohistorical) features of the Matese area. The selected geosites were associated to a new procedure to assess their Scientific Value (SV) and Potential Tourism Use (PTU), and to confirm their suitability for the purpose. To illustrate the geoitinerary, a geoitinerary map, and illustration material such as descriptive cards were produced. As an overall result, the proposed geoitinerary represents a valuable contribution for the geotourism promotion of the Matese Mountains on which to base future studies and initiatives in this perspective.